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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 4

sp. nov., isolated from saline soil of a paddy field Free

Abstract

Two novel aerobic bacteria, designated strains LAM0312 and LAM0313, were isolated from saline soil samples collected from a paddy field in Dezhou city, Shandong Province, China. Cells of these strains were Gram-stain-positive, sporogenous, rod-shaped and motile with peritrichous flagella. The optimal growth temperature and pH were 30 °C and pH 7.0–8.0. Strain LAM0312 was able to grow in the presence of 12 % (w/v) NaCl. The major fatty acids were anteiso-C and iso-C. The dominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, glycolipids, five unidentitied lipids and phosphatidylmonomethylethanolamine. The cell-wall peptidoglycan was found to contain -diaminopimelic acid. The predominant menaquinone was identified as menaquinone-7. The G+C content of the genomic DNA of strains LAM0312 and LAM0313 was 45.0 and 46.0 mol%, respectively, as determined by the method. 16S rRNA gene sequence similarity analysis indicated that the strains were closely related to DSM 25 and JCM 15716 with 98.5 and 96.4 % sequence similarity, respectively. The DNA–DNA hybridization value between strain LAM0312 and LAM0313 was 92±0.6 % (reciprocal 90±0.2 %) and the value between strain LAM0312 and DSM 25 was 48±0.5 % (reciprocal 40±0.4 %). On the basis of the phenotypic, phylogenetic and chemotaxonomic characteristics, the two strains are proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LAM0312 (=ACCC 06527=JCM 30849).

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Keyword(s): 16S rRNA gene , Brevibacillus halotolerans sp. nov. , paddy field , polyphasic taxonomy and saline soil
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2017-04-01
2023-09-25
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References

  1. Shida O, Takagi H, Kadowaki K, Komagata K. Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. Int J Syst Bacteriol 1996; 46:939–946 [View Article][PubMed]
     [Google Scholar]
  2. Logan NA, de Vos P. Genus IV. Brevibacillus Shida, Takagi, Kadowaki and Komagata 1996a, 942VP. In de Vos P, Garrity GM, Jones D, Krieg NR, Ludwig W. et al. (editors) Bergey’s Manual of Systematic Bacteriology 2nd edn, vol. 3 New York: Springer; 2009 pp. 304–316
     [Google Scholar]
  3. Logan NA, Forsyth G, Lebbe L, Goris J, Heyndrickx M et al. Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov. Int J Syst Evol Microbiol 2002; 52:953–966 [View Article][PubMed]
     [Google Scholar]
  4. Baek SH, Im WT, Oh HW, Lee JS, Oh HM et al. Brevibacillus ginsengisoli sp. nov., a denitrifying bacterium isolated from soil of a ginseng field. Int J Syst Evol Microbiol 2006; 56:2665–2669 [View Article][PubMed]
     [Google Scholar]
  5. Choi MJ, Bae JY, Kim KY, Kang H, Cha CJ. Brevibacillus fluminis sp. nov., isolated from sediment of estuarine wetland. Int J Syst Evol Microbiol 2010; 60:1595–1599 [View Article][PubMed]
     [Google Scholar]
  6. Takebe F, Hirota K, Nodasaka Y, Yumoto I. Brevibacillus nitrificans sp. nov., a nitrifying bacterium isolated from a microbiological agent for enhancing microbial digestion in sewage treatment tanks. Int J Syst Evol Microbiol 2012; 62:2121–2126 [View Article][PubMed]
     [Google Scholar]
  7. Inan K, Canakci S, Belduz AO, Sahin F. Brevibacillus aydinogluensis sp. nov., a moderately thermophilic bacterium isolated from Karakoc hot spring. Int J Syst Evol Microbiol 2012; 62:849–855 [View Article][PubMed]
     [Google Scholar]
  8. Hatayama K, Shoun H, Ueda Y, Nakamura A. Brevibacillus fulvus sp. nov., isolated from a compost pile. Int J Syst Evol Microbiol 2014; 64:506–512 [View Article][PubMed]
     [Google Scholar]
  9. Logan NA, Berge O, Bishop AH, Busse HJ, de Vos P et al. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 2009; 59:2114–2121 [View Article][PubMed]
     [Google Scholar]
  10. Ruan Z, Wang Y, Song J, Jiang S, Wang H et al. Kurthia huakuii sp. nov., isolated from biogas slurry, and emended description of the genus Kurthia. Int J Syst Evol Microbiol 2014; 64:518–521 [View Article][PubMed]
     [Google Scholar]
  11. Smibert RM, Krieg NR. Phenotypic characterization. In: Gerhare P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 647–654
     [Google Scholar]
  12. Smibert RM, Krieg NR. General characterization. In: Gerhare P, Murray RGE, Costilow RN, Nester EW, Wood WA. (editors) Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology; 1981 pp. 409–443
     [Google Scholar]
  13. Owens JJ. The egg yolk reaction produced by several species of bacteria. J Appl Bacteriol 1974; 37:137–148 [View Article][PubMed]
     [Google Scholar]
  14. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3:208–218 [View Article]
     [Google Scholar]
  15. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697–703 [View Article][PubMed]
     [Google Scholar]
  16. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
     [Google Scholar]
  17. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673–4680 [View Article][PubMed]
     [Google Scholar]
  18. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  19. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
     [Google Scholar]
  20. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
     [Google Scholar]
  21. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  22. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
     [Google Scholar]
  23. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962; 5:109–118 [View Article][PubMed]
     [Google Scholar]
  24. de Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970; 12:133–142 [View Article][PubMed]
     [Google Scholar]
  25. Huss VA, Festl H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 1983; 4:184–192 [View Article][PubMed]
     [Google Scholar]
  26. Sakamoto M, Suzuki M, Umeda M, Ishikawa I, Benno Y. Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensiscorrig., gen. nov., comb. nov. Int J Syst Evol Microbiol 2002; 52:841–849 [View Article][PubMed]
     [Google Scholar]
  27. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207 [CrossRef]
     [Google Scholar]
  28. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  29. Xu XW, Huo YY, Wang CS, Oren A, Cui HL et al. Pelagibacterium halotolerans gen. nov., sp. nov. and Pelagibacterium luteolum sp. nov., novel members of the family Hyphomicrobiaceae. Int J Syst Evol Microbiol 2011; 61:1817–1822 [View Article][PubMed]
     [Google Scholar]
  30. Fang MX, Zhang WW, Zhang YZ, Tan HQ, Zhang XQ et al. Brassicibacter mesophilus gen. nov., sp. nov., a strictly anaerobic bacterium isolated from food industry wastewater. Int J Syst Evol Microbiol 2012; 62:3018–3023 [View Article][PubMed]
     [Google Scholar]
  31. Schleifer KH. Analysis of the chemical composition and primary structure of murein. Meth Microbiol 1985; 18:123–156 [CrossRef]
     [Google Scholar]
  32. Kim MK, Sathiyaraj S, Pulla RK, Yang DC. Brevibacillus panacihumi sp. nov., a β-glucosidase-producing bacterium. Int J Syst Evol Microbiol 2009; 59:1227–1231 [View Article][PubMed]
     [Google Scholar]
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Brevibacillus halotolerans sp. nov., isolated from saline soil of a paddy field
Int J Syst Evol Microbiol 67, 772 (2017); https://doi.org/10.1099/ijsem.0.001579
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