1887

Abstract

Two novel aerobic bacteria, designated strains LAM0312 and LAM0313, were isolated from saline soil samples collected from a paddy field in Dezhou city, Shandong Province, China. Cells of these strains were Gram-stain-positive, sporogenous, rod-shaped and motile with peritrichous flagella. The optimal growth temperature and pH were 30 °C and pH 7.0–8.0. Strain LAM0312 was able to grow in the presence of 12 % (w/v) NaCl. The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0. The dominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, glycolipids, five unidentitied lipids and phosphatidylmonomethylethanolamine. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The predominant menaquinone was identified as menaquinone-7. The G+C content of the genomic DNA of strains LAM0312 and LAM0313 was 45.0 and 46.0 mol%, respectively, as determined by the T m method. 16S rRNA gene sequence similarity analysis indicated that the strains were closely related to Brevibacillus laterosporus DSM 25 and Brevibacillus fluminis JCM 15716 with 98.5 and 96.4 % sequence similarity, respectively. The DNA–DNA hybridization value between strain LAM0312 and LAM0313 was 92±0.6 % (reciprocal 90±0.2 %) and the value between strain LAM0312 and Brevibacillus laterosporus DSM 25 was 48±0.5 % (reciprocal 40±0.4 %). On the basis of the phenotypic, phylogenetic and chemotaxonomic characteristics, the two strains are proposed to represent a novel species of the genus Brevibacillus , for which the name Brevibacillus halotolerans sp. nov. is proposed. The type strain is LAM0312 (=ACCC 06527=JCM 30849).

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2017-05-05
2019-10-21
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