1887

Abstract

An antibiotic-producing, obligate anaerobic, Gram-stain-negative, catalase- and oxidase-negative strain (JC271) was isolated from a marine habitat and identified, based on 16S rRNA gene sequence analysis, as a novel member of the family . The closest phylogenetic relatives of strain JC271 were found to be C/L2 (99.2 %), W218 (98.7 %), subsp. (98.6 %), subsp. (98.0 %), subsp. (98.0 %) and other members of the genus (≤91.9 %). To resolve its full taxonomic position, the genomic sequence of strain JC271 was compared to available genomes of the most closely related phylogenetic members. Average Nucleotide Identity scores and DNA–DNA hybridization values confirmed that strain JC271 represents a novel genomic species. IsoC, isoCω9, and iso-C were found to be the major (comprising >10 % of the total present) fatty acids of strain JC271. Phosphatidylglycerol, phosphatidylethanolamine and unidentified lipids (L1-8) were the polar lipids identified. The G+C content of strain JC271 was 46.2 mol%. Integrated genomic and phenotypic data supported the classification of strain JC271 as a representative of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain is JC271 (=KCTC 15474=DSM 100016). It is also proposed that W218 is the latter heterotypic synonym of subsp. Sylt 3. subsp. Sylt 3 should also be elevated as comb. nov. and reclassified as comb. nov. subsp. should be reclassified as subsp. comb. nov. and subsp. as subsp. comb. nov.

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2017-01-01
2024-03-29
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