1887

Abstract

A novel actinomycete, designated strain m16, was isolated from a soil sample collected from the tropical rain forest of Xishuangbanna, a prefecture in Yunnan Province, south-west China, and characterized by using polyphasic taxomomy. Cells were aerobic and Gram-reaction-positive, and spore chains were observed to be of the helical type, with elliptical spores and smooth spore surfaces. The novel strain grew over a temperature range of 15–35 °C, at pH 5.0–11.0 and in the presence of 0–3 % (w/v) NaCl. The DNA G+C content of strain m16 was 70.0 mol%. The main fatty acids were iso-C (29.3 %), iso-C (15.4 %) and anteiso-C (14.6 %), and the predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). Comparative 16S rRNA gene sequence analysis showed that strain m16 was most closely related to KCTC 29262 (98.7 %), KACC 17632 (98.7 %), NBRC 108885 (98.5 %), DSM 42034 (98.4 %), JR-19 (98.4 %) and JR-43 (98.3 %). Phylogenetic, chemotaxonomic and phenotypic analyses indicated that strain m16 represents a novel species within the genus , for which the name is proposed. The type strain is m16 (=CGMCC 4.7110=KCTC 29195).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001573
2017-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/1/77.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001573&mimeType=html&fmt=ahah

References

  1. Waksman SA, Henrici AT. The nomenclature and classification of the actinomycetes. J Bacteriol 1943; 46:337–341[PubMed]
    [Google Scholar]
  2. Anderson AS, Wellington E. The taxonomy of Streptomyces and related genera. Int J Syst Evol Microbiol 2001; 51:797–814 [View Article][PubMed]
    [Google Scholar]
  3. Lechevalier MP, Lechevalier H. Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 1970; 20:435–443 [View Article]
    [Google Scholar]
  4. Bentley SD, Chater KF, Cerdeño-Tárraga AM, Challis GL, Thomson NR et al. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature 2002; 417:141–147 [View Article][PubMed]
    [Google Scholar]
  5. Ali N, Herron PR, Evans MC, Dyson PJ. Osmotic regulation of the Streptomyces lividans thiostrepton-inducible promoter, ptipA. Microbiology 2002; 148:381–390 [View Article][PubMed]
    [Google Scholar]
  6. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966; 16:313–340 [View Article]
    [Google Scholar]
  7. Jones KL. Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 1949; 57:141–145[PubMed]
    [Google Scholar]
  8. Dong X-Z, Cai M-Y. (editors) Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria Beijing: Science Press. (in Chinese); 2001 pp 370–398
    [Google Scholar]
  9. Kelly KL. Inter-Society Color Council - National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: USA Government Printing Office; 1964
    [Google Scholar]
  10. Shirling EB, Gottlieb D. Cooperative description of type cultures of Streptomyces. II. species descriptions from first study. Int J Syst Bacteriol 1968; 18:69–189 [View Article]
    [Google Scholar]
  11. Shirling EB, Gottlieb D. Cooperative description of type cultures of Streptomyces. III. additional species descriptions from first and second studies. Int J Syst Bacteriol 1968; 18:279–392 [View Article]
    [Google Scholar]
  12. Shirling EB, Gottlieb D. Cooperative description of type cultures of Streptomyces. IV. species descriptions from the second, third and fourth studies. Int J Syst Bacteriol 1969; 19:391–512 [View Article]
    [Google Scholar]
  13. Williams ST, Goodfellow M, Alderson G, Wellington EM, Sneath PH et al. Numerical classification of Streptomyces and related genera. J Gen Microbiol 1983; 129:1743–1813 [View Article][PubMed]
    [Google Scholar]
  14. Zhao XQ, Li WJ, Jiao WC, Li Y, Yuan WJ et al. Streptomyces xinghaiensis sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 2009; 59:2870–2874 [View Article][PubMed]
    [Google Scholar]
  15. Tarrand JJ, Gröschel DH. Rapid, modified oxidase test for oxidase-variable bacterial isolates. J Clin Microbiol 1982; 16:772–774[PubMed]
    [Google Scholar]
  16. Goodfellow M. Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 1971; 69:33–80 [View Article][PubMed]
    [Google Scholar]
  17. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  18. Hasegawa T, Takizawa M, Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 1983; 29:319–322 [View Article]
    [Google Scholar]
  19. Collins MD, Howarth OW, Grund E, Kroppenstedt RM. Isolation and structural determination of new members of the vitamin K2 series in Nocardia brasiliensis. FEMS Microbiol Lett 1987; 41:35–39 [View Article]
    [Google Scholar]
  20. Wu C, Lu X, Qin M, Wang Y, Ruan J. Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 1989; 16:176–178
    [Google Scholar]
  21. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  22. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962; 5:109–118 [View Article][PubMed]
    [Google Scholar]
  23. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R et al. Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 2007; 57:1424–1428 [View Article][PubMed]
    [Google Scholar]
  24. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  25. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4482 [View Article][PubMed]
    [Google Scholar]
  26. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  27. Kluge AG, Farris JS. Quantitative phyletics and the evolution of anurans. Syst Zool 1969; 18:1–32 [View Article]
    [Google Scholar]
  28. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  29. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  30. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  31. Huss VA, Festl H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 1983; 4:184–192 [View Article][PubMed]
    [Google Scholar]
  32. Jahnke K-D. BASIC computer program for evaluation of spectroscopic DNA renaturation data from GILFORD SYSTEM 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 1992; 15:61–73 [View Article]
    [Google Scholar]
  33. Labeda DP. DNADNA hybridization in the systematics of Streptomyces. Gene 1992; 115:249–253 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001573
Loading
/content/journal/ijsem/10.1099/ijsem.0.001573
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error