1887

Abstract

A novel actinomycete, designated strain KC-035, was isolated from soil collected from Krung Ching Waterfall National Park, Nakhon Si Thammarat Province, Thailand. Its taxonomic position was determined using a polyphasic approach. The strain had morphological and chemotaxonomic properties typical of members of the genus Streptomyces : flexuous spore chain; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H6) and MK-9(H4) as menaquinones; diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside as phospholipids; anteiso-C15 : 0, C16 : 0, iso-C16 : 0, iso-C15 : 0 and iso-C14 : 0 as major cellular fatty acids; and DNA G+C content of 72 mol%. 16S rRNA gene sequence analysis revealed that strain KC-035 showed high similarity to Streptomyces albiflavescens n20 (99.16 %) and Streptomyces siamensis KC-038 (98.43 %) as well as formed a monophyletic clade with them in the phylogenetic tree. On the basis of comparison of phenotypic properties and the low level of DNA–DNA relatedness, strain KC-035 could be distinguished from its closely related Streptomyces species and is considered to represent a novel species of the genus Streptomyces , for which the name Streptomyces krungchingensis sp. nov. is proposed. The type strain is KC-035 (=NBRC 110087=KCTC 29503=TISTR 2402).

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2017-02-20
2019-12-05
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References

  1. Bérdy J. Bioactive microbial metabolites. J Antibiot 2005;58:1–26 [CrossRef][PubMed]
    [Google Scholar]
  2. Sripreechasak P, Tamura T, Shibata C, Suwanborirux K, Tanasupawat S. Streptomyces andamanensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 2016;66:2030–2034 [CrossRef][PubMed]
    [Google Scholar]
  3. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16:313–340[CrossRef]
    [Google Scholar]
  4. Kelly KL. Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office; 1964
    [Google Scholar]
  5. Arai T. Culture Media for Actinomycetes Tokyo: The Society for Actinomycetes Japan; 1975
    [Google Scholar]
  6. Williams ST, Cross T. Actinomycetes. Methods Microbiol 1971;4:295–334[CrossRef]
    [Google Scholar]
  7. Becker B, Lechevalier MP, Lechevalier HA. Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 1965;13:236–243[PubMed]
    [Google Scholar]
  8. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977;100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  9. Uchida K, Aida K. An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 1984;37:463–464
    [Google Scholar]
  10. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinines and polar lipids. J Microbiol Methods 1984;2:233–241[CrossRef]
    [Google Scholar]
  11. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  12. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high performance liquid chromatography. FEMS Microbiol Lett 1984;25:125–128[CrossRef]
    [Google Scholar]
  13. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989;39:224–229[CrossRef]
    [Google Scholar]
  14. Takahashi Y, Matsumoto A, Seino A, Ueno J, Iwai Y et al. Streptomyces avermectinius sp. nov., an avermectin-producing strain. Int J Syst Evol Microbiol 2002;52:2163–2168 [CrossRef][PubMed]
    [Google Scholar]
  15. Lane DJ. 16S/23S rRNAsequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991; pp.115–148
    [Google Scholar]
  16. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16s rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  17. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  18. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376[PubMed][CrossRef]
    [Google Scholar]
  19. Kluge AG, Farris JS. Quantitative phyletics and the evolution of anurans. Syst Zool 1969;18:1–32[CrossRef]
    [Google Scholar]
  20. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. mega6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  21. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120[PubMed][CrossRef]
    [Google Scholar]
  22. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791[CrossRef]
    [Google Scholar]
  23. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International committee on systematic bacteriology. report of the ad hoc committee on the reconciliation of approaches to bacterial systematic. Int J Syst Bacteriol 1987;37:463–464[CrossRef]
    [Google Scholar]
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