1887

Abstract

The taxonomic position of a Gram-stain-positive, endospore-forming bacterium, strain KS16-9, isolated from a marine sediment sample collected from Kovalam, Kanyakumari coastal region of the Indian Ocean, India, was analysed by a polyphasic approach. Strain KS16-9 had typical phenotypic characteristics and chemotaxonomic features (menaquinones, fatty acids and lipids) that were consistent with the genus Bacillus . omparative analysis of the 16S rRNA gene sequence of the strain with previously published Bacillus type strains confirmed that it belongs to the genus Bacillus and is moderately related to Bacillus persicus B48 (98.42 % similarity), followed by Bacillus foraminis CV53 (97.67 %) and Bacillus rigiliprofundi (97.61 %). Other species in the genus Bacillus shared <97.6 % 16S rRNA gene sequence similarity. Strain KS16-9 contained MK-7 as the predominant menaquinone, meso-diaminopimelic acid as the diagnostic cell-wall diamino acid, iso-C15 : 0 and anteiso-C15 : 0 as major fatty acids, and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major phospholipids. The DNA G+C content of strain KS16-9 was 45.4 mol%. Based on data from this polyphasic taxonomic study, strain KS16-9 represents a novel species of the genus Bacillus , for which the name Bacillus maritimus sp. nov. is proposed. The type strain is KS16-9 (=MTCC 12305=DSM 100413=KCTC 33834).

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2017-02-20
2019-10-21
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References

  1. Zhang J, Wang J, Fang C, Song F, Xin Y et al. Bacillus oceanisediminis sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 2010;60:2924–2929 [CrossRef][PubMed]
    [Google Scholar]
  2. Ash C, Farrow JAE, Wallbanks S, Collins MD. Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett Appl Microbiol 1991;13:202–206 [CrossRef]
    [Google Scholar]
  3. Ash C, Priest FG, Collins MD. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie Van Leeuwenhoek 1993;64:253–260[PubMed][CrossRef]
    [Google Scholar]
  4. Shida O, Takagi H, Kadowaki K, Komagata K. Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. Int J Syst Bacteriol 1996;46:939–946 [CrossRef][PubMed]
    [Google Scholar]
  5. Heyndrickx M, Lebbe L, Kersters K, de Vos P, Forsyth G et al. Virgibacillus: a new genus to accommodate bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. Int J Syst Bacteriol 1998;48:99–106 [CrossRef]
    [Google Scholar]
  6. Wainø M, Tindall BJ, Schumann P, Ingvorsen K. Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 1999;49 Pt 2:821–831 [CrossRef][PubMed]
    [Google Scholar]
  7. Nazina TN, Tourova TP, Poltaraus AB, Novikova EV, Grigoryan AA et al. Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans. Int J Syst Evol Microbiol 2001;51:433–446 [CrossRef][PubMed]
    [Google Scholar]
  8. Yoon JH, Lee KC, Weiss N, Kho YH, Kang KH et al. Sporosarcina aquimarina sp. nov., a bacterium isolated from seawater in Korea, and transfer of Bacillus globisporus (Larkin and Stokes 1967), Bacillus psychrophilus (Nakamura 1984) and Bacillus pasteurii (Chester 1898) to the genus Sporosarcina as Sporosarcina globispora comb. nov., Sporosarcina psychrophila comb. nov. and Sporosarcina pasteurii comb. nov., and emended description of th. Int J Syst Evol Microbiol 2001;51:1079–1086 [CrossRef][PubMed]
    [Google Scholar]
  9. Albert RA, Archambault J, Lempa M, Hurst B, Richardson C et al. Proposal of Viridibacillus gen. nov. and reclassification of Bacillus arvi, Bacillus arenosi and Bacillus neidei as Viridibacillus arvi gen. nov., comb. nov., Viridibacillus arenosi comb. nov. and Viridibacillus neidei comb. nov. Int J Syst Evol Microbiol 2007;57:2729–2737 [CrossRef][PubMed]
    [Google Scholar]
  10. Ahmed I, Yokota A, Yamazoe A, Fujiwara T. Proposal of Lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov. Int J Syst Evol Microbiol 2007;57:1117–1125 [CrossRef][PubMed]
    [Google Scholar]
  11. Yoon JH, Kang SJ, Oh TK. Reclassification of Marinococcus albus Hao et al. 1985 as Salimicrobium album gen. nov., comb. nov. and Bacillus halophilus Ventosa et al. 1990 as Salimicrobium halophilum comb. nov., and description of Salimicrobium luteum sp. nov. Int J Syst Evol Microbiol 2007;57:2406–2411 [CrossRef][PubMed]
    [Google Scholar]
  12. Vaishampayan P, Miyashita M, Ohnishi A, Satomi M, Rooney A et al. Description of Rummeliibacillus stabekisii gen. nov., sp. nov. and reclassification of Bacillus pycnus Nakamura et al. 2002 as Rummeliibacillus pycnus comb. nov. Int J Syst Evol Microbiol 2009;59:1094–1099 [CrossRef][PubMed]
    [Google Scholar]
  13. Krishnamurthi S, Chakrabarti T, Stackebrandt E. Re-examination of the taxonomic position of Bacillus silvestris Rheims et al. 1999 and proposal to transfer it to Solibacillus gen. nov. as Solibacillus silvestris comb. nov. Int J Syst Evol Microbiol 2009;59:1054–1058 [CrossRef][PubMed]
    [Google Scholar]
  14. Zhou Y, Xu J, Xu L, Tindall BJ. Falsibacillus pallidus to replace the homonym Bacillus pallidus Zhou et al. 2008. Int J Syst Evol Microbiol 2009;59:3176–3180 [CrossRef][PubMed]
    [Google Scholar]
  15. Krishnamurthi S, Ruckmani A, Pukall R, Chakrabarti T. Psychrobacillus gen. nov. and proposal for reclassification of Bacillus insolitus Larkin & Stokes, 1967, B. psychrotolerans Abd-El Rahman et al., 2002 and B. psychrodurans Abd-El Rahman et al., 2002 as Psychrobacillus insolitus comb. nov., Psychrobacillus psychrotolerans comb. nov. and Psychrobacillus psychrodurans comb. nov. Syst Appl Microbiol 2010;33:367–373 [CrossRef][PubMed]
    [Google Scholar]
  16. Zavarzina DG, Tourova TP, Kolganova T V, Boulygina ES, Zhilina TN. Description of Anaerobacillus alkalilacustre gen. nov., sp. nov. strictly anaerobic diazotrophic bacillus isolated from soda lake and transfer of Bacillus arseniciselenatis, Bacillus macyae, and Bacillus alkalidiazotrophicus to Anaerobacillus as the new combinations A. arseniciselenatis comb. nov., A. macyae comb. nov., and A. alkalidiazotrophicus comb. nov. Microbiol 2009;78:723–731[CrossRef]
    [Google Scholar]
  17. Coorevits A, Dinsdale AE, Halket G, Lebbe L, de Vos P et al. Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly 'thermoglucosidasius'); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov. Int J Syst Evol Microbiol 2012;62:1470–1485 [CrossRef][PubMed]
    [Google Scholar]
  18. Didari M, Amoozegar MA, Bagheri M, Schumann P, Spröer C et al. Alteribacillus bidgolensis gen. nov., sp. nov., a moderately halophilic bacterium from a hypersaline lake, and reclassification of Bacillus persepolensis as Alteribacillus persepolensis comb. nov. Int J Syst Evol Microbiol 2012;62:2691–2697 [CrossRef][PubMed]
    [Google Scholar]
  19. Kämpfer P, Glaeser SP, Busse HJ. Transfer of Bacillus schlegelii to a novel genus and proposal of Hydrogenibacillus schlegelii gen. nov., comb. nov. Int J Syst Evol Microbiol 2013;63:1723–1727 [CrossRef][PubMed]
    [Google Scholar]
  20. Boeye H, Aerts M. Numerical taxonomy of Bacillus isolates from North Sea sediments. Int J Syst Bacteriol 1976;26:427–441[CrossRef]
    [Google Scholar]
  21. Claus D, Berkeley RCW. Genus Bacillus, Cohn 1872. In Sneath PHA, Mair NS, Sharpe ME, Holt JG. (editors) Bergey’s Manual of Systematic Bacteriologyvol. 2 Baltimore: The Williams and Wilkins Co; 1986; pp.1105–1139
    [Google Scholar]
  22. Ortigosa M, Garay E, Pujalte M-J. Gram-positive bacteria of marine origin: a numerical taxonomic study on Mediterranean isolates. Microbiología SEM 1997;13:543–462
    [Google Scholar]
  23. Nowlan B, Dodia MS, Singh SP, Patel BK. Bacillus okhensis sp. nov., a halotolerant and alkalitolerant bacterium from an Indian saltpan. Int J Syst Evol Microbiol 2006;56:1073–1077 [CrossRef][PubMed]
    [Google Scholar]
  24. Dastager SG, Mawlankar R, Mual P, Verma A, Krishnamurthi S et al. Bacillus encimensis sp. nov. isolated from marine sediment. Int J Syst Evol Microbiol 2015;65:1421–1425 [CrossRef][PubMed]
    [Google Scholar]
  25. Kumar RM, Kaur G, Kumar A, Bala M, Singh NK et al. Taxonomic description and genome sequence of Bacillus campisalis sp. nov., a member of the genus Bacillus isolated from a solar saltern. Int J Syst Evol Microbiol 2015;65:3235–3240 [CrossRef][PubMed]
    [Google Scholar]
  26. Logan NA, Berge O, Bishop AH, Busse HJ, De Vos P et al. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 2009;59:2114–2121 [CrossRef][PubMed]
    [Google Scholar]
  27. Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA. et al (editors) Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology; 1981
    [Google Scholar]
  28. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhard P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  29. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 2005;55:1149–1153 [CrossRef][PubMed]
    [Google Scholar]
  30. Smith NR, Gordon RE, Clark FE. Aerobic spore forming bacteria Washington, DC: Agriculture Monograph No. 16, United States Department of Agriculture; 1952
    [Google Scholar]
  31. Mayilraj S, Saha P, Suresh K, Saini HS. Ornithinimicrobium kibberense sp. nov., isolated from the Indian Himalayas. Int J Syst Evol Microbiol 2006;56:1657–1661 [CrossRef][PubMed]
    [Google Scholar]
  32. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  33. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  34. Kumar A, Bajaj A, Kumar RM, Kaur G, Kaur N et al. Taxonomic description and Genome sequence of Rheinheimera mesophila sp. nov., strain IITR-13T isolated from a pesticide contaminated sandy soil. Int J Syst Evol Microbiol 2015;65:3666–3673[CrossRef]
    [Google Scholar]
  35. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974;28:226–231[PubMed]
    [Google Scholar]
  36. Schumann P. Peptidoglycan structure. Methods Microbiol 2011;38:101–129[CrossRef]
    [Google Scholar]
  37. Collins MD, Jones D. Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 1981;45:316–354[PubMed]
    [Google Scholar]
  38. Wu C, Lu X, Qin M, Wang Y, Ruan J. Analysis of menaquinone compound in microbial cells by HPLC. Microbiology 1989;16:176–178
    [Google Scholar]
  39. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  40. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  41. Kämpfer P, Kroppenstedt RM. Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 1996;42:989–1005[CrossRef]
    [Google Scholar]
  42. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962;5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  43. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 1994;44:846–849[CrossRef]
    [Google Scholar]
  44. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006;33:152–155
    [Google Scholar]
  45. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014;64:346–351 [CrossRef][PubMed]
    [Google Scholar]
  46. Didari M, Amoozegar MA, Bagheri M, Mehrshad M, Schumann P et al. Bacillus persicus sp. nov., a halophilic bacterium from a hypersaline lake. Int J Syst Evol Microbiol 2013;63:1229–1234 [CrossRef][PubMed]
    [Google Scholar]
  47. Tiago I, Pires C, Mendes V, Morais PV, Da Costa MS et al. Bacillus foraminis sp. nov., isolated from a non-saline alkaline groundwater. Int J Syst Evol Microbiol 2006;56:2571–2574 [CrossRef][PubMed]
    [Google Scholar]
  48. Mccarthy BJ, Bolton ET. An approach to the measurement of genetic relatedness among organisms. Proc Natl Acad Sci USA 1963;50:156–164 [CrossRef][PubMed]
    [Google Scholar]
  49. Sylvan JB, Hoffman CL, Momper LM, Toner BM, Amend JP et al. Bacillus rigiliprofundi sp. nov., an endospore-forming, Mn-oxidizing, moderately halophilic bacterium isolated from deep subseafloor basaltic crust. Int J Syst Evol Microbiol 2015;65:1992–1998 [CrossRef][PubMed]
    [Google Scholar]
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