1887

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Volume 67, Issue 2

gen. nov., sp. nov., of the family isolated from paddy soil Free

Abstract

A polyphasic approach was used to characterize a novel bacterium, designated strain TPP412, isolated from a paddy soil in Taiwan. Strain TPP412 was Gram-stain-negative, facultatively anaerobic, rod-shaped, motile with a single polar flagellum and lacked bacteriochlorophyll. Growth was observed at 24–45 °C (optimal 25 °C), at pH 5.0–10.0 (optimal pH 7.0) and with 0–0.75 % (w/v) NaCl. Strain TPP412 showed highest 16S rRNA gene sequence similarity to members of the genera (94.1–94.5 %), (93.9–94.5 %) and (93.4–94.4 %) and established a discrete taxonomic lineage in phylogenetic analysis. The major fatty acids found in strain TPP412 were C, C 3-OH, iso-C 3-OH, C, C 7/C 6 and C 7/C 6. The major polar lipids consisted of phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unidentified lipid. The polyamine pattern showed a predominance of putrescine and a minor amount of spermidine. The DNA G+C content was 58.4 mol% and the predominant quinone system was ubiquinone-8 (Q-8). The low 16S rRNA gene sequence similarity values (≤94.5%) and distinct phylogenetic clustering clearly distinguished strain TPP412 from other representatives of the family Based on the discrete phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence analysis, strain TPP412 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is TPP412 (=BCRC 80905=JCM 30814).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Oryzomicrobium terrae , Propionivibrio limicola , Rhodocyclaceae and Strain TPP412T
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2017-02-01
2024-04-19
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Oryzomicrobium terrae gen. nov., sp. nov., of the family Rhodocyclaceae isolated from paddy soil
Int J Syst Evol Microbiol 67, 183 (2017); https://doi.org/10.1099/ijsem.0.001565
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