1887

Abstract

A Gram-stain-negative, non-motile, aerobic and coccoid, ovoid or rod-shaped bacterium, designated GHTF-24, was isolated from a tidal flat on the South Sea, South Korea, and subjected to polyphasic taxonomic study. The novel strain grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain GHTF-24 fell within the clade comprising the type strains of species of the genus Roseovarius . Strain GHTF-24 exhibited 16S rRNA gene sequence similarity values of 97.0 % to each of Roseovarius halotolerans HJ50 and Roseovarius pacificus 81-2 and of 91.6–96.5 % to the type strains of the other species of the genus Roseovarius . Strain GHTF-24 contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c as the major fatty acid. The major polar lipids of strain GHTF-24 were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain GHTF-24 was 62.3 mol%, and its mean DNA–DNA relatedness values with the type strains of R. halotolerans and R. pacificus were 13–16 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GHTF-24 is separated from other recognized species of the genus Roseovarius . On the basis of the data presented, strain GHTF-24 is considered to represent a novel species of the genus Roseovarius , for which the name Roseovarius aestuariivivens sp. nov. is proposed. The type strain is GHTF-24 (=KCTC 52454=NBRC 112420).

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2017-02-06
2019-10-18
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