1887

Abstract

A Gram-stain-negative, rod-shaped, non-flagellated, pink, cold-tolerant bacterial strain, NH7-4, was isolated from the Riganqiao peatlands on the Tibetan Plateau. The 16S rRNA gene sequence of the novel isolate shared a pairwise similarity ranging from 96.84 to 93.02 % with type strains of species of the genus . Growth of strain NH7-4 occurred between 0 and 30 °C and at pH 5.0–9.0, with an optimum growth temperature at 20 °C and an optimum pH for growth of approximately 7.0. The major isoprenoid quinone was MK-7. The major cellular fatty acids were summed feature 3 (Cω7 and/or iso-C 2-OH), iso-C, iso-C 3-OH, C, iso-C 3-OH and Cω5. The major polar lipid of strain NH7-4 was phosphatidylethanolamine. Strain NH7-4 did not assimilate any substrates in API 20NE strips without low concentrations of yeast extract being present and had a lower optimal growth temperature, which distinguished it from other type strains of species of the genus . The DNA G+C content of strain NH7-4 was 48.6 mol%. Based on phylogenetic, phenotypic and chemotaxonomic data, strain NH7-4 (=JCM 30607=CGMCC1.14937) represents a novel species of the genus for which the name sp. nov. is proposed.

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2017-04-01
2024-03-29
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