A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated RA4-7T, was isolated from the gut of an abalone (Haliotis discus hannai) collected from the sea around Jeju island, South Korea, and subjected to a polyphasic taxonomic study. RA4-7T grew optimally at 25 °C, at pH 7.0–7.5 and in the presence of 2.0–3.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that RA4-7T represented a member of the genus Polaribacter. RA4-7T exhibited 16S rRNA gene sequence similarity values of 97.64 and 97.23 % to Polaribacter atrinae WP25T and Polaribacter dokdonensis DSW-5T, respectively, and of 93.83–96.99 % to the type strains of the other species of the genus Polaribacter. RA4-7T contained MK-6 as the predominant menaquinone and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 3-OH and iso-C15 : 0as the major fatty acids. The major polar lipids detected in RA4-7T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of RA4-7T was 30.5 mol% and its DNA–DNA relatedness values with the type strains of P. atrinae and P. dokdonensis were 16 and 11 %, respectively. Differential phenotypic properties, in combination with its phylogenetic and genetic distinctiveness, revealed that RA4-7T is separated from species of the genus Polaribacter with validly published names. On the basis of the data presented, RA4-7T represented a novel species of the genus Polaribacter, for which the name Polaribacterhaliotis sp. nov. is proposed. The type strain is RA4-7T (=KCTC 52418T=NBRC 112383T).
BarrowG.I,
FelthamR. K. A.1993Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [CrossRef]
BernardetJ.-F.2011; Family I. Flavobacteriaceae Reichenbach 1992. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 4 pp. 106–111 Edited by
KriegN. R.,
LudwigW.,
WhitmanW. B.,
HedlundB. P.,
PasterB. J.,
StaleyJ. T.,
WardN.,
BrownD.,
ParteA.
New York: Springer;
BernardetJ. F.,
NakagawaY.,
HolmesB.Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
BowmanJ. P.2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868 [View Article][PubMed]
BrunsA.,
RohdeM.,
Berthe-CortiL.2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
CLSI2012Performance Standards for Antimicrobial Disk Susceptibility Tests Approved Standard , 11th edn.M02–A11 Wayne, PA: Clinical and Laboratory Standards Institute;
EmbleyT. M.,
WaitR.1994; Structural lipids of eubacteria. In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics pp. 121–161 Edited by
GoodfellowM.,
O’DonnellA. G.
Chichester: John Wiley & Sons;
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
FukuiY.,
AbeM.,
KobayashiM.,
SaitoH.,
OikawaH.,
YanoY.,
SatomiM.2013; Polaribacter porphyrae sp. nov., isolated from the red alga Porphyra yezoensis, and emended descriptions of the genus Polaribacter and two Polaribacter species. Int J Syst Evol Microbiol 63:1665–1672 [View Article][PubMed]
GosinkJ. J.,
WoeseC. R.,
StaleyJ. T.1998; Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga–Flavobacterium–Bacteroides group and reclassification of ‘Flectobacillus glomeratus' as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48:223–235 [View Article][PubMed]
HyunD. W.,
ShinN. R.,
KimM. S.,
KimP. S.,
JungM. J.,
KimJ. Y.,
WhonT. W.,
BaeJ. W.2014; Polaribacter atrinae sp. nov., isolated from the intestine of a comb pen shell, Atrina pectinata
. Int J Syst Evol Microbiol 64:1654–1661 [View Article][PubMed]
KimB. C.,
OhH. W.,
KimH.,
ParkD. S.,
HongS. G.,
LeeH. K.,
BaeK. S.2013; Polaribacter sejongensis sp. nov., isolated from Antarctic soil, and emended descriptions of the genus Polaribacter, Polaribacter butkevichii and Polaribacter irgensii
. Int J Syst Evol Microbiol 63:4000–4005 [View Article][PubMed]
MinnikinD. E.,
O'DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
NedashkovskayaO. I.,
KimS. B.,
LysenkoA. M.,
KalinovskayaN. I.,
MikhailovV. V.,
KimI. S.,
BaeK. S.2005; Polaribacter butkevichii sp. nov., a novel marine mesophilic bacterium of the family Flavobacteriaceae
. Curr Microbiol 51:408–412 [View Article][PubMed]
NedashkovskayaO. I.,
KukhlevskiyA. D.,
ZhukovaN. V.2013; Polaribacter reichenbachii sp. nov.: a new marine bacterium associated with the green alga Ulva fenestrata. Curr Microbiol 66:16–21 [View Article][PubMed]
ParkS.,
ParkJ. M.,
JungY. T.,
LeeK. C.,
LeeJ. S.,
YoonJ. H.2014a; Polaribacter marinivivus sp. nov., a member of the family Flavobacteriaceae isolated from seawater. Antonie Van Leeuwenhoek 106:1139–1146 [View Article][PubMed]
ReichenbachH.1992; The order Cytophagales
. In The Prokaryotes. a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn. pp. 3631–3675 Edited by
BalowsA.,
TrüperH. G.,
DworkinM.,
HarderW.,
SchleiferK. H.
New York: Springer;
StackebrandtE.,
GoebelB. M.1994; Taxonomic Note: A place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Microbiol 44:846–849 [View Article]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM.I,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E. et al.1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]
YoonJ.-H.,
KangK. H.,
ParkY.-H.2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [View Article][PubMed]