1887

Abstract

A Gram-stain-positive bacterium, strain LZ-22, was isolated from a rhizosphere of moss Leptobryum sp. collected at the shore of Lake Zub in Antarctica. Cells were motile, straight or pleomorphic rods with sizes of 0.6–1.0×3.5–10 µm. The novel isolate was a facultatively anaerobic, catalase-positive, psychrotolerant mesophile. Growth was observed at 3–41 °C (optimum 24–28 °C), with 0–7 % (w/v) NaCl (optimum 0.25 %) and at pH 4.0–9.0 (optimum pH 7.8). The quinone system of strain LZ-22 possessed predominately menaquinone MK-9(H4). The genomic G+C content was 70.2 mol%. Strain 10J was isolated from a biofilm of sediment microbial fuel cell, in Uruguay and had 99 % 16S rRNA gene sequence similarity to strain LZ-22. DNA–DNA-hybridization values of 84 % confirmed that both strains belonged to the same species. Both strains grew on sugars, proteinaceous compounds, and some amino- and organic acids. Strain LZ-22 uniquely grew on D-enantiomers of histidine and valine while neglecting growth on L-enantiomers. Both strains were sensitive to most of the tested antibiotics but resistant to tested nitrofurans and sulfanilamides. Phylogenetic analyses of the 16S rRNA gene sequences indicated that the strains were related to members of the family Propionibacteriaceae (~93–94 % 16S rRNA gene sequence similarity) with formation of a separate branch within the radiation of the genera Granulicoccus and Luteococcus . Based on phenotypic and genotypic characteristics, we propose the affiliation of both strains into a novel species of a new genus. The name Raineyella antarctica gen. nov., sp. nov. is proposed for the novel taxon with the type strain LZ-22 (=ATCC TSD-18=DSM 100494=JCM 30886).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001552
2016-12-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5529.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001552&mimeType=html&fmt=ahah

References

  1. Alonso-Vega P., Carro L., Martínez-Molina E., Trujillo M. E..( 2011;). Auraticoccus monumenti gen. nov., sp. nov., an actinomycete isolated from a deteriorated sandstone monument. . Int J Syst Evol Microbiol 61: 1098–1103. [CrossRef] [PubMed]
    [Google Scholar]
  2. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J..( 1996;). Classification of bacteria isolated from a medieval wall painting. . J Biotechnol 47: 39–52. [CrossRef]
    [Google Scholar]
  3. Barrow G. I., Feltham R. K. A..( 1993;). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;.[CrossRef]
    [Google Scholar]
  4. Clinical and Laboratory Standards Institute( 2015;). Performance standards for antimicrobial susceptibility testing; Twenty-fifth informational supplement. . CLSI document M100-S25.
  5. Collins M. D., Lawson P. A., Nikolaitchouk N., Falsen E..( 2000;). Luteococcus peritonei sp. nov., isolated from the human peritoneum. . Int J Syst Evol Microbiol 50: 179–181. [CrossRef] [PubMed]
    [Google Scholar]
  6. da Costa M. S., Rainey F. A., Moe W. M..( 2015;). Brooklawnia. . Bergey's Manual of Systematics of Archaea and Bacteria, pp. 1–5. Hoboken, NJ:: Wiley;.
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A..( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12: 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  8. Gillis M., De Ley J., De Cleene M..( 1970;). The determination of molecular weight of bacterial genome DNA from renaturation rates. . Eur J Biochem 12: 143–153. [CrossRef] [PubMed]
    [Google Scholar]
  9. Guisler M., Pikuta E. V, Townsend A., Hoover R. B..( 2009;). Psychrotolerant anaerobes from Lake Podprudnoye, Antarctica and penguin Spheniscus demersus colony, South Africa. . In Instruments, Methods, and Missions for Astrobiology XII,vol. 7441 pp. 74410L-1–7441012. Edited by Hoover R. B., Levin G. V., Rozanov A. Y., Davies P. C. W.. Bellingham, WA:: SPIE;.
    [Google Scholar]
  10. Hoover R. B..( 2008;). Schirmacher Oasis/Lake Untersee Antarctica Astrobiology Expeditions. Explorer's Club Expedition Report Flag #162. . https://explorers.org/flag_reports/Flag_162_Report_-_Richard_B._Hoover_9-29-08_.pdf.
  11. Hoover R. B., Pikuta E. V..( 2009;). Psychrophilic and psychrotolerant microbial extremophiles in polar environments. . In Polar Microbiology: The Ecology, Biodiversity and Bioremediation Potential of Microorganisms in Extremely Cold Environments, pp. 115–156. Edited by Bej Asim K., Aislabie J., Atlas Ronald M.. Boca Raton:: Taylor & Francis Group;.[CrossRef]
    [Google Scholar]
  12. Kämpfer P., Kroppenstedt R. M..( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42: 989–1005. [CrossRef]
    [Google Scholar]
  13. Kevbrin V. V., Zavarzin G. A..( 1992;). Effect of sulfur compounds on the growth of the halophilic homoacetic bacterium Acetohalobium arabaticum. . Mikrobiologiia 61: 812–817.
    [Google Scholar]
  14. Kuykendall L. D., Roy M. A., O'neill J. J., Devine T. E..( 1988;). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. . Int J Syst Bacteriol 38: 358–361. [CrossRef]
    [Google Scholar]
  15. Loveland-Curtze J., Miteva V. I., Brenchley J. E..( 2011;). Evaluation of a new fluorimetric DNA-DNA hybridization method. . Can J Microbiol 57: 250–255. [CrossRef] [PubMed]
    [Google Scholar]
  16. Marmur J..( 1961;). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3: 208–218. [CrossRef]
    [Google Scholar]
  17. Maszenan A. M., Jiang H. L., Tay J.-H., Schumann P., Kroppenstedt R. M., Tay S. T.-L..( 2007;). Granulicoccus phenolivorans gen. nov., sp. nov., a Gram-positive, phenol-degrading coccus isolated from phenol-degrading aerobic granules. . Int J Syst Evol Microbiol 57: 730–737. [CrossRef] [PubMed]
    [Google Scholar]
  18. Menes R. J., Muxí L..( 2002;). Anaerobaculum mobile sp. nov., a novel anaerobic, moderately thermophilic, peptide-fermenting bacterium that uses crotonate as an electron acceptor, and emended description of the genus Anaerobaculum. . Int J Syst Evol Microbiol 52: 157–164. [CrossRef] [PubMed]
    [Google Scholar]
  19. Menes R. J., Viera C. E., Farías M. E., Seufferheld M. J..( 2011;). Halomonas vilamensis sp. nov., isolated from high-altitude Andean lakes. . Int J Syst Evol Microbiol 61: 1211–1217. [CrossRef] [PubMed]
    [Google Scholar]
  20. Miller L. T..( 1982;). A single derivatization method for bacterial fatty acid methyl esters including hydroxy acids. . J Clin Microbiol 16: 584–586.
    [Google Scholar]
  21. Nakamura K., Hiraishi A., Yoshimi Y., Kawaharasaki M., Masuda K., Kamagata Y..( 1995;). Microlunatus phosphovorus gen. nov., sp. nov., a new Gram-positive polyphosphate-accumulating bacterium isolated from activated sludge. . Int J Syst Bacteriol 45: 17–22. [CrossRef] [PubMed]
    [Google Scholar]
  22. Patrick S., McDowell A..( 2015;). Propionibacterium. . In Bergey's Manual of Systematics of Archaea and Bacteria, pp. 1–29. Wiley;.
    [Google Scholar]
  23. Pikuta E. V., Hoover R. B..( 2010;). Utilization of alternate chirality enantiomers in microbial communities. . In Instruments, Methods, and Missions for Astrobiology XIII,vol. 7819 pp. 78190P-78190P-14. Edited by Hoover R. B., Levin G. V., Rozanov A. Y., Davies P. C. W.. Bellingham, WA:: SPIE;.[CrossRef]
    [Google Scholar]
  24. Pikuta E. V, Hoover R. B..( 2014;). The Genus Carnobacterium. . In Lactic Acid Bacteria: Biodiversity and Taxonomy, pp. 107–123. Edited by Holzapfel W. H., Wood B. J. B.. Chichester, UK:: John Wiley & Sons;.[CrossRef]
    [Google Scholar]
  25. Pikuta E. V, Hoover R. B., Klyce B., Davies P. C. W., Davies P..( 2006a;). Bacterial utilization of L-sugars and D-amino acids. . In Instruments, Methods, and Missions for Astrobiology IX,vol. 6309 p. 63090A. Edited by Hoover R. B., Levin G. V., Rozanov A. Yu.. Bellingham, WA:: SPIE;.[CrossRef]
    [Google Scholar]
  26. Pikuta E. V., Itoh T., Krader P., Tang J., Whitman W. B., Hoover R. B..( 2006b;). Anaerovirgula multivorans gen. nov., sp. nov., a novel spore-forming, alkaliphilic anaerobe isolated from Owens Lake, California, USA. . Int J Syst Evol Microbiol 56: 2623–2629. [CrossRef] [PubMed]
    [Google Scholar]
  27. Pitcher D. G., Collins M. D..( 1991;). Phylogenetic analysis of some ll-diaminopimelic acid-containing coryneform bacteria from human skin: description of Propionibacterium innocuum sp. nov. . FEMS Microbiol Lett 84: 295–300. [CrossRef]
    [Google Scholar]
  28. Price M. N., Dehal P. S., Arkin A. P..( 2010;). FastTree 2-approximately maximum-likelihood trees for large alignments. . PLoS One 5: e9490. [CrossRef] [PubMed]
    [Google Scholar]
  29. Schriek S., Rückert C., Staiger D., Pistorius E. K., Michel K. P..( 2007;). Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803. . BMC Genomics 8: 437. [CrossRef] [PubMed]
    [Google Scholar]
  30. Seshadri S..( 2015;). Aestuariimicrobium. . In Bergey's Manual of Systematics of Archaea and Bacteria, pp. 1–2. Wiley;.
    [Google Scholar]
  31. Seviour R. J., Maszenan A. M..( 2015;). Tessaracoccus. . In Bergey's Manual of Systematics of Archaea and Bacteria, pp. 1–4. Wiley;.
    [Google Scholar]
  32. Smibert R. M., Krieg N. R..( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P.. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  33. Stackebrandt E..( 2015;). Propionimicrobium. . In Bergey's Manual of Systematics of Archaea and Bacteria, pp. 1–4. Wiley;.
    [Google Scholar]
  34. Stolz A., Busse H. J., Kämpfer P..( 2007;). Pseudomonas knackmussii sp. nov. . Int J Syst Evol Microbiol 57: 572–576. [CrossRef] [PubMed]
    [Google Scholar]
  35. Stookey L. L..( 1970;). Ferrozine – a new spectrophotometric reagent for iron. . Anal Chem 42: 779–781. [CrossRef]
    [Google Scholar]
  36. Tamura K., Nei M., Kumar S..( 2004;). Prospects for inferring very large phylogenies by using the neighbor-joining method. . Proc Natl Acad Sci U S A 101: 11030–11035. [CrossRef]
    [Google Scholar]
  37. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30: 2725–2729. [CrossRef] [PubMed]
    [Google Scholar]
  38. Tindall B. J..( 1990a;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66: 199–202. [CrossRef]
    [Google Scholar]
  39. Tindall B. J..( 1990b;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13: 128–130. [CrossRef]
    [Google Scholar]
  40. Townsend A., Pikuta E. V., Guisler M., Stahl S., Hoover R. B..( 2009;). Anaerobic psychrophiles from Lake Zub and Lake Untersee, Antarctica. . In Instruments and Methods for Astrobiology and Planetary Missions XII,vol. 7441 pp. 74410K-1–7474410. Edited by Retherford K. D., Hoover R. B., Levin G. V., Rozanov A. Yu.. Bellingham, WA:: SPIE;.[CrossRef]
    [Google Scholar]
  41. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I, Moore L. H., Moore W. E. C., Murray R. G. E. et al.( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37: 463–464.[CrossRef]
    [Google Scholar]
  42. Wolin E. A., Wolin M. J., Wolfe R. S..( 1963;). Formation of methane by bacterial extracts. . J Biol Chem 238: 2882–2886.[PubMed]
    [Google Scholar]
  43. Yarza P., Yilmaz P., Pruesse E., Glöckner F. O., Ludwig W., Schleifer K. H., Whitman W. B., Euzéby J., Amann R., Rosselló-Móra R..( 2014;). Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. . Nat Rev Microbiol 12: 635–645. [CrossRef] [PubMed]
    [Google Scholar]
  44. Yokota A..( 2015;). Propioniferax. . In Bergey's Manual of Systematics of Archaea and Bacteria, pp. 1–4. Wiley;.
    [Google Scholar]
  45. Yokota A., Tamura T., Takeuchi M., Weiss N., Stackebrandt E..( 1994;). Transfer of Propionibacterium innocuum Pitcher and Collins 1991 to Propioniferax gen. nov. as Propioniferax innocua comb. nov. . Int J Syst Bacteriol 44: 579–582. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001552
Loading
/content/journal/ijsem/10.1099/ijsem.0.001552
Loading

Data & Media loading...

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error