@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001547, author = "Techo, Sujitra and Miyashita, Mika and Shibata, Chiyo and Tanaka, Naoto and Wisetkhan, Preeyarach and Visessanguan, Wonnop and Tanasupawat, Somboon", title = "Lactobacillus ixorae sp. nov., isolated from a flower (West-Indian jasmine)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "12", pages = "5500-5505", doi = "https://doi.org/10.1099/ijsem.0.001547", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001547", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "lactic acid bacteria", keywords = "flower", keywords = "heterofermentative", keywords = "Lactobacillus ixorae", abstract = "A Gram-stain-positive, lactic acid bacterium, strain Ru20-1T, was isolated from a flower (West-Indian jasmine) collected from Kalasin province, Thailand. A polyphasic approach was used to determine the taxonomic position of this strain. Studies of morphological and biochemical characteristics revealed that strain Ru20-1T belonged to the genus Lactobacillus . The strain was heterofermentative, non-spore-forming and rod-shaped. It produced dl-lactic acid. Based on 16S rRNA gene sequence similarity, this strain was closely related to Lactobacillus lindneri LMG 14528T (96.8 %), Lactobacillus sanfranciscensis NRIC 1548T (95.4 %) and Lactobacillus florum NRIC 0771T (95.2 %), respectively. In addition, the pheS gene sequence of strain Ru20-1T was closely related to those of L. sanfranciscensis NRIC 1548T (92.0 %), L. lindneri LMG 14528T (89.0 %) and L. florum NRIC 0771T(85.0 %). Phylogenetic analysis indicated that strain Ru20-1T was clearly separated from closely related species of the genus Lactobacillus . The DNA G+C content of strain Ru20-1T was 47.8 mol %. The cell-wall peptidoglycan type was l-Lys–d-Asp. The major cellular fatty acids were C18 : 1ω9c, C20 : 0, C20 : 1ω9c and summed feature 7 (unknown 18.846 and/or C19 : 1ω6c and/or C19 : 0 cyclo). On the basis of the data provided, strain Ru20-1T represents a novel species of the genus Lactobacillus , for which the name Lactobacillus ixorae sp. nov. is proposed. The type strain is Ru20-1T (=LMG 29008T=NBRC 111239T=PCU 346T=TISTR 2381T).", }