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Abstract

A Gram-stain-positive, lactic acid bacterium, strain Ru20-1, was isolated from a flower (West-Indian jasmine) collected from Kalasin province, Thailand. A polyphasic approach was used to determine the taxonomic position of this strain. Studies of morphological and biochemical characteristics revealed that strain Ru20-1 belonged to the genus . The strain was heterofermentative, non-spore-forming and rod-shaped. It produced -lactic acid. Based on 16S rRNA gene sequence similarity, this strain was closely related to LMG 14528 (96.8 %), NRIC 1548 (95.4 %) and NRIC 0771 (95.2 %), respectively. In addition, the gene sequence of strain Ru20-1 was closely related to those of NRIC 1548 (92.0 %), LMG 14528 (89.0 %) and NRIC 0771(85.0 %). Phylogenetic analysis indicated that strain Ru20-1 was clearly separated from closely related species of the genus . The DNA G+C content of strain Ru20-1 was 47.8 mol %. The cell-wall peptidoglycan type was -Lys–-Asp. The major cellular fatty acids were Cω9, C, Cω9 and summed feature 7 (unknown 18.846 and/or Cω6 and/or C cyclo). On the basis of the data provided, strain Ru20-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Ru20-1 (=LMG 29008=NBRC 111239=PCU 346=TISTR 2381).

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2016-12-01
2024-03-28
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