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Abstract

A Gram-stain-positive, lactic acid bacterium, strain Ru20-1, was isolated from a flower (West-Indian jasmine) collected from Kalasin province, Thailand. A polyphasic approach was used to determine the taxonomic position of this strain. Studies of morphological and biochemical characteristics revealed that strain Ru20-1 belonged to the genus . The strain was heterofermentative, non-spore-forming and rod-shaped. It produced -lactic acid. Based on 16S rRNA gene sequence similarity, this strain was closely related to LMG 14528 (96.8 %), NRIC 1548 (95.4 %) and NRIC 0771 (95.2 %), respectively. In addition, the gene sequence of strain Ru20-1 was closely related to those of NRIC 1548 (92.0 %), LMG 14528 (89.0 %) and NRIC 0771(85.0 %). Phylogenetic analysis indicated that strain Ru20-1 was clearly separated from closely related species of the genus . The DNA G+C content of strain Ru20-1 was 47.8 mol %. The cell-wall peptidoglycan type was -Lys–-Asp. The major cellular fatty acids were Cω9, C, Cω9 and summed feature 7 (unknown 18.846 and/or Cω6 and/or C cyclo). On the basis of the data provided, strain Ru20-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Ru20-1 (=LMG 29008=NBRC 111239=PCU 346=TISTR 2381).

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2016-12-01
2020-09-25
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References

  1. De Man J. C., Rogosa M., Sharpe M. E.. 1960; A medium for the cultivation of lactobacilli. J Appl Bacteriol23:130–135 [CrossRef]
    [Google Scholar]
  2. Endo A., Futagawa-Endo Y., Sakamoto M., Kitahara M., Dicks L. M. T.. 2010; Lactobacillus florum sp. nov., a fructophilic species isolated from flowers. Int J Syst Evol Microbiol60:2478–2482 [CrossRef]
    [Google Scholar]
  3. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–791 [CrossRef]
    [Google Scholar]
  4. Hammes W. P., Hertel C.. 2009; Genus Lactobacillus. In Bergey’s Manual of Systematic Bacteriology, 2nd edn.vol. 3 pp.464–532 Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B.. New York: Springer;
    [Google Scholar]
  5. Hucker G. J., Conn H. J.. 1923; Method of gram staining. N Y St Agric Exp Sta Tech Bull93:3–37
    [Google Scholar]
  6. Kämpfer P., Kroppenstedt R. M.. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol42:989–1005 [CrossRef]
    [Google Scholar]
  7. Kawasaki S., Kurosawa K., Miyazaki M., Yagi C., Kitajima Y., Tanaka S., Irisawa T., Okada S., Sakamoto M. et al. 2011a; Lactobacillus floricola sp. nov., lactic acid bacteria isolated from mountain flowers. Int J Syst Evol Microbiol61:1356–1359 [CrossRef]
    [Google Scholar]
  8. Kawasaki S., Kurosawa K., Miyazaki M., Sakamoto M., Ohkuma M., Niimura Y.. 2011b; Lactobacillus ozensis sp. nov., isolated from mountain flowers. Int J Syst Evol Microbiol61:2435–2438 [CrossRef]
    [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  10. Kimura M.. 1980; A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120 [CrossRef]
    [Google Scholar]
  11. Lane D. J.. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp.115–175 Edited by Stackebrandt E., Goodfellow M.. Chichester: Wiley;
    [Google Scholar]
  12. Miyagawa E., Azuma R., Suto T.. 1981; Peptidoglycan composition of gram-negative obligately anaerobic rods. J Gen Appl Microbiol27:199–208 [CrossRef]
    [Google Scholar]
  13. Mundt J. O., Hammer J. L.. 1968; Lactobacilli on plants. Appl Microbiol16:1326–1330[PubMed]
    [Google Scholar]
  14. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J.. 2005; Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiol151:2141–2150 [CrossRef]
    [Google Scholar]
  15. Nei M., Kumar S.. 2000; Molecular Evolution and Phylogenetics New York: Oxford University Press;
    [Google Scholar]
  16. Okada S., Toyoda T., Kozaki M.. 1978; An easy method for the determination of the optical types of lactic acid produced by lactic acid bacteria. Agric Biol Chem42:1781–1783
    [Google Scholar]
  17. Phalip V., Schmitt P., Diviès C.. 1994; A method for screening diacetyl and acetoin-producing bacteria on agar plates. J Basic Microbiol34:277–280 [CrossRef]
    [Google Scholar]
  18. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  19. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
    [Google Scholar]
  20. Tamaoka J.. 1994; Determination of DNA base compositon. In Chemical Methods in Prokaryotic Systematics pp.463–470 Edited by Goodfellow M., O’Donnell A. G.. Chichester: Wiley;
    [Google Scholar]
  21. Tamaoka J., Komagata K.. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett25:125–128 [CrossRef]
    [Google Scholar]
  22. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  23. Tanasupawat S., Thongsanit J., Okada S., Komagata K.. 2002; Lactic acid bacteria isolated from soy sauce mash in Thailand. J Gen Appl Microbiol48:201–209 [CrossRef]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  25. Yamada K., Komagata K.. 1970; Taxonomic studies on coryneform bacteria. III. DNA base composition of coryneform bacteria. J Gen Appl Microbiol16:215–224 [CrossRef]
    [Google Scholar]
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