1887

Abstract

A Gram-stain-positive, lactic acid bacterium, strain Ru20-1, was isolated from a flower (West-Indian jasmine) collected from Kalasin province, Thailand. A polyphasic approach was used to determine the taxonomic position of this strain. Studies of morphological and biochemical characteristics revealed that strain Ru20-1 belonged to the genus Lactobacillus . The strain was heterofermentative, non-spore-forming and rod-shaped. It produced dl-lactic acid. Based on 16S rRNA gene sequence similarity, this strain was closely related to Lactobacillus lindneri LMG 14528 (96.8 %), Lactobacillus sanfranciscensis NRIC 1548 (95.4 %) and Lactobacillus florum NRIC 0771 (95.2 %), respectively. In addition, the pheS gene sequence of strain Ru20-1 was closely related to those of L. sanfranciscensis NRIC 1548 (92.0 %), L. lindneri LMG 14528 (89.0 %) and L. florum NRIC 0771(85.0 %). Phylogenetic analysis indicated that strain Ru20-1 was clearly separated from closely related species of the genus Lactobacillus . The DNA G+C content of strain Ru20-1 was 47.8 mol %. The cell-wall peptidoglycan type was l-Lys–d-Asp. The major cellular fatty acids were C18 : 1ω9c, C20 : 0, C20 : 1ω9c and summed feature 7 (unknown 18.846 and/or C19 : 1ω6c and/or C19 : 0 cyclo). On the basis of the data provided, strain Ru20-1 represents a novel species of the genus Lactobacillus , for which the name Lactobacillus ixorae sp. nov. is proposed. The type strain is Ru20-1 (=LMG 29008=NBRC 111239=PCU 346=TISTR 2381).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001547
2016-12-01
2019-08-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5500.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001547&mimeType=html&fmt=ahah

References

  1. De Man J. C., Rogosa M., Sharpe M. E..( 1960;). A medium for the cultivation of lactobacilli. . J Appl Bacteriol23:130–135. [CrossRef]
    [Google Scholar]
  2. Endo A., Futagawa-Endo Y., Sakamoto M., Kitahara M., Dicks L. M. T..( 2010;). Lactobacillus florum sp. nov., a fructophilic species isolated from flowers. . Int J Syst Evol Microbiol60:2478–2482. [CrossRef]
    [Google Scholar]
  3. Felsenstein J..( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution39:783–791. [CrossRef]
    [Google Scholar]
  4. Hammes W. P., Hertel C..( 2009;). Genus Lactobacillus. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn.,vol. 3 pp. 464–532. Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B.. New York:: Springer;.
    [Google Scholar]
  5. Hucker G. J., Conn H. J..( 1923;). Method of gram staining. . N Y St Agric Exp Sta Tech Bull93:3–37.
    [Google Scholar]
  6. Kämpfer P., Kroppenstedt R. M..( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol42:989–1005. [CrossRef]
    [Google Scholar]
  7. Kawasaki S., Kurosawa K., Miyazaki M., Yagi C., Kitajima Y., Tanaka S., Irisawa T., Okada S., Sakamoto M. et al.( 2011a;). Lactobacillus floricola sp. nov., lactic acid bacteria isolated from mountain flowers. . Int J Syst Evol Microbiol61:1356–1359. [CrossRef]
    [Google Scholar]
  8. Kawasaki S., Kurosawa K., Miyazaki M., Sakamoto M., Ohkuma M., Niimura Y..( 2011b;). Lactobacillus ozensis sp. nov., isolated from mountain flowers. . Int J Syst Evol Microbiol61:2435–2438. [CrossRef]
    [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol62:716–721. [CrossRef][PubMed]
    [Google Scholar]
  10. Kimura M..( 1980;). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16:111–120. [CrossRef]
    [Google Scholar]
  11. Lane D. J..( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M.. Chichester:: Wiley;.
    [Google Scholar]
  12. Miyagawa E., Azuma R., Suto T..( 1981;). Peptidoglycan composition of gram-negative obligately anaerobic rods. . J Gen Appl Microbiol27:199–208. [CrossRef]
    [Google Scholar]
  13. Mundt J. O., Hammer J. L..( 1968;). Lactobacilli on plants. . Appl Microbiol16:1326–1330.[PubMed]
    [Google Scholar]
  14. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J..( 2005;). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiol151:2141–2150. [CrossRef]
    [Google Scholar]
  15. Nei M., Kumar S..( 2000;). Molecular Evolution and Phylogenetics. New York:: Oxford University Press;.
    [Google Scholar]
  16. Okada S., Toyoda T., Kozaki M..( 1978;). An easy method for the determination of the optical types of lactic acid produced by lactic acid bacteria. . Agric Biol Chem42:1781–1783.
    [Google Scholar]
  17. Phalip V., Schmitt P., Diviès C..( 1994;). A method for screening diacetyl and acetoin-producing bacteria on agar plates. . J Basic Microbiol34:277–280. [CrossRef]
    [Google Scholar]
  18. Saitou N., Nei M..( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol4:406–425.[PubMed]
    [Google Scholar]
  19. Sasser M..( 1990;). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, , MIDI Technical Note 101.. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  20. Tamaoka J..( 1994;). Determination of DNA base compositon. . In Chemical Methods in Prokaryotic Systematics, pp. 463–470. Edited by Goodfellow M., O’Donnell A. G.. Chichester:: Wiley;.
    [Google Scholar]
  21. Tamaoka J., Komagata K..( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett25:125–128. [CrossRef]
    [Google Scholar]
  22. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol30:2725–2729. [CrossRef][PubMed]
    [Google Scholar]
  23. Tanasupawat S., Thongsanit J., Okada S., Komagata K..( 2002;). Lactic acid bacteria isolated from soy sauce mash in Thailand. . J Gen Appl Microbiol48:201–209. [CrossRef]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G..( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res25:4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  25. Yamada K., Komagata K..( 1970;). Taxonomic studies on coryneform bacteria. III. DNA base composition of coryneform bacteria. . J Gen Appl Microbiol16:215–224. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001547
Loading
/content/journal/ijsem/10.1099/ijsem.0.001547
Loading

Data & Media loading...

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error