1887

Abstract

Coccoid green algae are extremely diverse despite their simple coccoid phenotype, a phenotype that may be the result of convergent evolution. In this study, we used a polyphasic approach combining molecular phylogenetic analyses, morphology and ultrastructure to investigate isolated coccoid strains from China, and our results reveal three new lineages of Trebouxiophyceae: the novel genus and species Mysteriochloris nanningensis gen. et sp. nov., and the two novel species Phyllosiphon coccidium sp. nov. and Desertella yichangensis sp. nov. (Trebouxiophyceae, Chlorophyta). We provide a detailed characterization of the novel microalgae which they are autosporic coccoid unicells and have parietal chloroplasts. In phylogenies based on 18S rDNA sequences and the chloroplast ribulose-bisphosphate carboxylase gene (rbcL), these three algae are nested within the Watanabea clade and are different from any known algae. M. nanningensis FACHB-1787 is not really close to any known algae within the Watanabea clade. Phyllosiphoncoccidium FACHB-2212 is within the Phyllosiphon lineages. D. yichangensis FACHB-1793 is closely related to Desertella californica and described as a representative of a novel species of the genus Desertella.

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2016-12-01
2019-09-22
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References

  1. Aboal M., Werner O..( 2011;). Morphology, fine structure, life cycle and phylogenetic analysis of Phyllosiphon arisari, a siphonous parasitic green alga. . Eur J Phycol 46: 181–192. [CrossRef]
    [Google Scholar]
  2. Albertano P., Pollio A., Taddei R..( 1991;). Viridiella fridericiana (Chlorococcales, Chlorophyta), a new genus and species isolated from extremely acid environments. . Phycologia 30: 346–354. [CrossRef]
    [Google Scholar]
  3. Aslam Z., Shin W., Kim M. K., Im W. T., Lee S. T..( 2007;). Marinichlorella kaistiae gen. et sp. nov. (Trebouxiophyceae, Chlorophyta) based on polyphasic taxonomy. . J Phycol 43: 576–584.[CrossRef]
    [Google Scholar]
  4. Bock C., Krienitz L., Proschold T..( 2011;). Taxonomic reassessment of the genus Chlorella (Trebouxiophyceae) using molecular signatures (barcodes), including description of seven new species. . Fottea 11: 293–312. [CrossRef]
    [Google Scholar]
  5. Darienko T., Gustavs L., Mudimu O., Menendez C. R..( 2010;). Chloroidium, a common terrestrial coccoid green alga previously assigned to Chlorella (Trebouxiophyceae, Chlorophyta). . Eur J Phycol 45: 79–95. [CrossRef]
    [Google Scholar]
  6. Fučíková K., Leliaert F., Cooper E. D., Å kaloud P., D'Hondt S., De Clerck O., Gurgel C. F. D., Lewis L. A., Lewis P. O. et al.( 2014a;). New phylogenetic hypotheses for the core chlorophyta based on chloroplast sequence data. . Front Ecol Evol 2: 1–12.
    [Google Scholar]
  7. Fučíková K., Lewis L. A., Lewis P. A..( 2014b;). Widespread desert affiliation of trebouxiophycean algae (Trebouxiophyceae, Chlorophyta) including discovery of three new desert genera. . Phycological Res 62: 294–305.[CrossRef]
    [Google Scholar]
  8. Hallmann C., Stannek L., Fritzlar D., Hause-Reitner D., Friedl T., Hoppert M..( 2013;). Molecular diversity of phototrophic biofilms on building stone. . FEMS Microbiol Ecol 84: 355–372. [CrossRef] [PubMed]
    [Google Scholar]
  9. Hanagata N., Karube I., Chihara M., Silva P. C..( 1998;). Reconsideration of the taxonomy of ellipsoidal species of Chlorella (Trebouxiophyceae, Chlorophyta), with establishment of Watanabea sen. nov. . Phycol Res 46: 221–229. [CrossRef]
    [Google Scholar]
  10. Karsten U., Friedl T., Schumann R., Hoyer K., Lembcke S..( 2005;). Mycosporing-like amino acids and phylogenies in green algae: Prasiola and ITS relatives from the Trebouxiophyceae (Chlorophyta). . J Phycol 41: 557–566.[CrossRef]
    [Google Scholar]
  11. Katoh K., Kuma K., Toh H., Miyata T..( 2005;). MAFFT version 5: improvement in accuracy of multiple sequence alignment. . Nucleic Acids Res 33: 511–518. [CrossRef] [PubMed]
    [Google Scholar]
  12. Krienitz L., Hegewald E. H., Hepperle D., Wolf M..( 2003;). The systematics of coccoid green algae: 18S rRNA gene sequence data versus morphology. . Biologia 58: 437–446
    [Google Scholar]
  13. Krienitz L., Huss V. A. R., Bock C..( 2015;). Chlorella: 125 years of the green survivalist. . Trends Plant Sci 20: 67–69. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kulichová J., Škaloud P., Neustupa J..( 2014;). Molecular diversity of green corticolous microalgae from two sub-mediterranean European localities. . Eur J Phycol 49: 345–355. [CrossRef]
    [Google Scholar]
  15. Kühn J..( 1878;). über eine neue parasitische alge phyllosiphon arisari welche die Laubblätter einer terrestrischen Pflanze ganz in derselben Weise befällt, wie dies von parasitischen Pilzen bekanntist. . Sitzungsber Naturf Ges Halle 2: 24–26.
    [Google Scholar]
  16. Leliaert F., Smith D. R., Moreau H., Herron M. D., Verbruggen H., Delwiche C. F., De Clerck O..( 2012;). Phylogeny and molecular evolution of the green algae. . Crit Rev Plant Sci 31: 1–46.[CrossRef]
    [Google Scholar]
  17. Lewin R. A., Krienitz L., Goericke R., Takeda H., Hepperle D..( 2000;). Picocystis salinarum gen. et sp. nov. (Chlorophyta) – a new picoplanktonic green alga. . Phycologia 39: 560–565. [CrossRef]
    [Google Scholar]
  18. Luo W., Pröschold T., Bock C., Krienitz L..( 2010;). Generic concept in Chlorella-related coccoid green algae (Chlorophyta, Trebouxiophyceae). . Plant Biol 12: 545–553. [CrossRef] [PubMed]
    [Google Scholar]
  19. Ma S., Huss V. A. R., Tan D., Sun X., Chen J., Xie Y., Zhang J..( 2013;). A novel species in the genus Heveochlorella (Trebouxiophyceae, Chlorophyta) witnesses the evolution from an epiphytic into an endophytic lifestyle in tree-dwelling green algae. . Eur J Phycol 48: 200–209. [CrossRef]
    [Google Scholar]
  20. Medlin L., Elwood H. J., Stickel S., Sogin M. L..( 1988;). The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. . Gene 71: 491–499. [CrossRef]
    [Google Scholar]
  21. Nadson G..( 1906;). K morphologii nizshix vodoroslej [Zur Morphologie der niederen Algen.]. . Izv Imp S-Peterburgsk Bot Sada 6: 184–194.
    [Google Scholar]
  22. Neustupa J., Němcová Y., Eliáš M., Škaloud P..( 2009;). Kalinella bambusicola gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel coccoid Chlorella-like subaerial alga from Southeast Asia. . Phycological Res 57: 159–169. [CrossRef]
    [Google Scholar]
  23. Neustupa J., Eliáš M., Škaloud P., Němcová Y., Šejnohová L..( 2011;). Xylochloris irregularis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga. . Phycologia 50: 57–66. [CrossRef]
    [Google Scholar]
  24. Neustupa J., Nemcova Y., Vesela J., Steinova J., Skaloud P..( 2013a;). Leptochlorella corticola gen. et sp. nov. and Kalinella apyrenoidosa sp. nov.: two novel Chlorella-like green microalgae (Trebouxiophyceae, Chlorophyta) from subaerial habitats. . Int J Syst Evol Microbiol 63: 377–387. [CrossRef] [PubMed]
    [Google Scholar]
  25. Neustupa J., Němcová Y., Veselá J., Steinová J., Škaloud P..( 2013b;). Parachloroidium gen. nov. (Trebouxiophyceae, Chlorophyta), a novel genus of coccoid green algae from subaerial corticolous biofilms. . Phycologia 52: 411–421. [CrossRef]
    [Google Scholar]
  26. Nyati S., Beck A., Honegger R..( 2007;). Fine structure and phylogeny of green algal photobionts in the microfilamentous genus Psoroglaena (Verrucariaceae, lichen-forming ascomycetes). . Plant Biol 9: 390–399. [CrossRef] [PubMed]
    [Google Scholar]
  27. Nylander J. A. A..( 2004;). MrModeltest 2.1. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden:.
    [Google Scholar]
  28. Němcová Y., Eliáš M., Škaloud P., Hodač L., Neustupa J..( 2011;). Jenufa gen. nov. a new genus of coccoid green algae (Chlorophyceae, Incertae Sedis) previously recorded by environmental sequencing. . J Phycol 47: 928–938.[CrossRef]
    [Google Scholar]
  29. Posada D., Crandall K. A..( 1998;). Modeltest: testing the model of DNA substitution. . Bioinformatics 14: 817–818.[CrossRef]
    [Google Scholar]
  30. Procházková K., Nmcová Y., Kulichová J., Neustupa J..( 2015;). Morphology and phylogeny of parasitic and free-living members of the genus Phyllosiphon (Trebouxiophyceae, Chlorophyta). . Nova Hedwigia 101: 501–518. [CrossRef]
    [Google Scholar]
  31. Reynolds C. S..( 2007;). Variability in the provision and function of mucilage in phytoplankton: facultative responses to the environment. . Hydrobiologia 578: 37–45. [CrossRef]
    [Google Scholar]
  32. Ronquist F., Huelsenbeck J. P..( 2003;). MrBayes 3: Bayesian phylogenetic inference under mixed models. . Bioinformatics 19: 1572–1574. [CrossRef] [PubMed]
    [Google Scholar]
  33. Song H., Zhang Q., Liu G., Hu Z..( 2015;). Polulichloris henanensis gen. et sp. nov.(Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga. . Phytotaxa 218: 137–146.[CrossRef]
    [Google Scholar]
  34. Swofford D. L..( 2003;). paup*. Phylogenetic analysis using parsimony (* and other methods). Version 4. Sinauer Associates;. Sunderland, MA:.
  35. Štifierová A., Neustupa J..( 2015;). Community structure of corticolous microalgae within a single forest stand: evaluating the effects of bark surface pH and tree species. . Fottea 15: 113–122.[CrossRef]
    [Google Scholar]
  36. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30: 2725–2729. [CrossRef] [PubMed]
    [Google Scholar]
  37. Zechman F. W..( 2003;). Phylogeny of the dasycladales (Chlorophyta, Ulvophyceae) based on analyses of RUBISCO large subunit (rbcL) gene sequences. . J Phycol 39: 819–827.[CrossRef]
    [Google Scholar]
  38. Zhang J., Huss V. A. R., Sun X., Chang K., Pang D..( 2008;). Morphology and phylogenetic position of a trebouxiophycean green alga (Chlorophyta) growing on the rubber tree, Hevea brasiliensis, with the description of a new genus and species. . Eur J Phycol 43: 185–193. [CrossRef]
    [Google Scholar]
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