1887

Abstract

Coccoid green algae are extremely diverse despite their simple coccoid phenotype, a phenotype that may be the result of convergent evolution. In this study, we used a polyphasic approach combining molecular phylogenetic analyses, morphology and ultrastructure to investigate isolated coccoid strains from China, and our results reveal three new lineages of Trebouxiophyceae: the novel genus and species gen. et sp. nov., and the two novel species sp. nov. and sp. nov. (Trebouxiophyceae, Chlorophyta). We provide a detailed characterization of the novel microalgae which they are autosporic coccoid unicells and have parietal chloroplasts. In phylogenies based on 18S rDNA sequences and the chloroplast ribulose-bisphosphate carboxylase gene (L), these three algae are nested within the clade and are different from any known algae. FACHB-1787 is not really close to any known algae within the clade. FACHB-2212 is within the lineages. FACHB-1793 is closely related to and described as a representative of a novel species of the genus .

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2016-12-01
2024-03-29
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References

  1. Aboal M., Werner O. 2011; Morphology, fine structure, life cycle and phylogenetic analysis of Phyllosiphon arisari, a siphonous parasitic green alga. Eur J Phycol 46:181–192 [View Article]
    [Google Scholar]
  2. Albertano P., Pollio A., Taddei R. 1991; Viridiella fridericiana (Chlorococcales, Chlorophyta), a new genus and species isolated from extremely acid environments. Phycologia 30:346–354 [View Article]
    [Google Scholar]
  3. Aslam Z., Shin W., Kim M. K., Im W. T., Lee S. T. 2007; Marinichlorella kaistiae gen. et sp. nov. (Trebouxiophyceae, Chlorophyta) based on polyphasic taxonomy. J Phycol 43:576–584 [CrossRef]
    [Google Scholar]
  4. Bock C., Krienitz L., Proschold T. 2011; Taxonomic reassessment of the genus Chlorella (Trebouxiophyceae) using molecular signatures (barcodes), including description of seven new species. Fottea 11:293–312 [View Article]
    [Google Scholar]
  5. Darienko T., Gustavs L., Mudimu O., Menendez C. R. 2010; Chloroidium, a common terrestrial coccoid green alga previously assigned to Chlorella (Trebouxiophyceae, Chlorophyta). Eur J Phycol 45:79–95 [View Article]
    [Google Scholar]
  6. Fučíková K., Leliaert F., Cooper E. D., Å kaloud P., D'Hondt S., De Clerck O., Gurgel C. F. D., Lewis L. A., Lewis P. O. et al. 2014a; New phylogenetic hypotheses for the core chlorophyta based on chloroplast sequence data. Front Ecol Evol 2:1–12
    [Google Scholar]
  7. Fučíková K., Lewis L. A., Lewis P. A. 2014b; Widespread desert affiliation of trebouxiophycean algae (Trebouxiophyceae, Chlorophyta) including discovery of three new desert genera. Phycological Res 62:294–305 [CrossRef]
    [Google Scholar]
  8. Hallmann C., Stannek L., Fritzlar D., Hause-Reitner D., Friedl T., Hoppert M. 2013; Molecular diversity of phototrophic biofilms on building stone. FEMS Microbiol Ecol 84:355–372 [View Article][PubMed]
    [Google Scholar]
  9. Hanagata N., Karube I., Chihara M., Silva P. C. 1998; Reconsideration of the taxonomy of ellipsoidal species of Chlorella (Trebouxiophyceae, Chlorophyta), with establishment of Watanabea sen. nov. Phycol Res 46:221–229 [View Article]
    [Google Scholar]
  10. Karsten U., Friedl T., Schumann R., Hoyer K., Lembcke S. 2005; Mycosporing-like amino acids and phylogenies in green algae: Prasiola and ITS relatives from the Trebouxiophyceae (Chlorophyta). J Phycol 41:557–566 [CrossRef]
    [Google Scholar]
  11. Katoh K., Kuma K., Toh H., Miyata T. 2005; MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Res 33:511–518 [View Article][PubMed]
    [Google Scholar]
  12. Krienitz L., Hegewald E. H., Hepperle D., Wolf M. 2003; The systematics of coccoid green algae: 18S rRNA gene sequence data versus morphology. Biologia 58:437–446
    [Google Scholar]
  13. Krienitz L., Huss V. A. R., Bock C. 2015; Chlorella: 125 years of the green survivalist. Trends Plant Sci 20:67–69 [View Article][PubMed]
    [Google Scholar]
  14. Kulichová J., Škaloud P., Neustupa J. 2014; Molecular diversity of green corticolous microalgae from two sub-mediterranean European localities. Eur J Phycol 49:345–355 [View Article]
    [Google Scholar]
  15. Kühn J. 1878; über eine neue parasitische alge phyllosiphon arisari welche die Laubblätter einer terrestrischen Pflanze ganz in derselben Weise befällt, wie dies von parasitischen Pilzen bekanntist. Sitzungsber Naturf Ges Halle 2:24–26
    [Google Scholar]
  16. Leliaert F., Smith D. R., Moreau H., Herron M. D., Verbruggen H., Delwiche C. F., De Clerck O. 2012; Phylogeny and molecular evolution of the green algae. Crit Rev Plant Sci 31:1–46 [CrossRef]
    [Google Scholar]
  17. Lewin R. A., Krienitz L., Goericke R., Takeda H., Hepperle D. 2000; Picocystis salinarum gen. et sp. nov. (Chlorophyta) – a new picoplanktonic green alga. Phycologia 39:560–565 [View Article]
    [Google Scholar]
  18. Luo W., Pröschold T., Bock C., Krienitz L. 2010; Generic concept in Chlorella-related coccoid green algae (Chlorophyta, Trebouxiophyceae). Plant Biol 12:545–553 [View Article][PubMed]
    [Google Scholar]
  19. Ma S., Huss V. A. R., Tan D., Sun X., Chen J., Xie Y., Zhang J. 2013; A novel species in the genus Heveochlorella (Trebouxiophyceae, Chlorophyta) witnesses the evolution from an epiphytic into an endophytic lifestyle in tree-dwelling green algae. Eur J Phycol 48:200–209 [View Article]
    [Google Scholar]
  20. Medlin L., Elwood H. J., Stickel S., Sogin M. L. 1988; The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71:491–499 [View Article]
    [Google Scholar]
  21. Nadson G. 1906; K morphologii nizshix vodoroslej [Zur Morphologie der niederen Algen.]. Izv Imp S-Peterburgsk Bot Sada 6:184–194
    [Google Scholar]
  22. Neustupa J., Němcová Y., Eliáš M., Škaloud P. 2009; Kalinella bambusicola gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel coccoid Chlorella-like subaerial alga from Southeast Asia. Phycological Res 57:159–169 [View Article]
    [Google Scholar]
  23. Neustupa J., Eliáš M., Škaloud P., Němcová Y., Šejnohová L. 2011; Xylochloris irregularis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga. Phycologia 50:57–66 [View Article]
    [Google Scholar]
  24. Neustupa J., Nemcova Y., Vesela J., Steinova J., Skaloud P. 2013a; Leptochlorella corticola gen. et sp. nov. and Kalinella apyrenoidosa sp. nov.: two novel Chlorella-like green microalgae (Trebouxiophyceae, Chlorophyta) from subaerial habitats. Int J Syst Evol Microbiol 63:377–387 [View Article][PubMed]
    [Google Scholar]
  25. Neustupa J., Němcová Y., Veselá J., Steinová J., Škaloud P. 2013b; Parachloroidium gen. nov. (Trebouxiophyceae, Chlorophyta), a novel genus of coccoid green algae from subaerial corticolous biofilms. Phycologia 52:411–421 [View Article]
    [Google Scholar]
  26. Nyati S., Beck A., Honegger R. 2007; Fine structure and phylogeny of green algal photobionts in the microfilamentous genus Psoroglaena (Verrucariaceae, lichen-forming ascomycetes). Plant Biol 9:390–399 [View Article][PubMed]
    [Google Scholar]
  27. Nylander J. A. A. 2004 MrModeltest 2.1 Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Uppsala Sweden:
    [Google Scholar]
  28. Němcová Y., Eliáš M., Škaloud P., Hodač L., Neustupa J. 2011; Jenufa gen. nov. a new genus of coccoid green algae (Chlorophyceae, Incertae Sedis) previously recorded by environmental sequencing. J Phycol 47:928–938 [CrossRef]
    [Google Scholar]
  29. Posada D., Crandall K. A. 1998; Modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818 [CrossRef]
    [Google Scholar]
  30. Procházková K., Nmcová Y., Kulichová J., Neustupa J. 2015; Morphology and phylogeny of parasitic and free-living members of the genus Phyllosiphon (Trebouxiophyceae, Chlorophyta). Nova Hedwigia 101:501–518 [View Article]
    [Google Scholar]
  31. Reynolds C. S. 2007; Variability in the provision and function of mucilage in phytoplankton: facultative responses to the environment. Hydrobiologia 578:37–45 [View Article]
    [Google Scholar]
  32. Ronquist F., Huelsenbeck J. P. 2003; MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574 [View Article][PubMed]
    [Google Scholar]
  33. Song H., Zhang Q., Liu G., Hu Z. 2015; Polulichloris henanensis gen. et sp. nov.(Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga. Phytotaxa 218:137–146 [CrossRef]
    [Google Scholar]
  34. Swofford D. L. 2003 paup*. Phylogenetic analysis using parsimony (* and other methods) Version 4. Sinauer Associates; Sunderland, MA:
  35. Štifierová A., Neustupa J. 2015; Community structure of corticolous microalgae within a single forest stand: evaluating the effects of bark surface pH and tree species. Fottea 15:113–122 [CrossRef]
    [Google Scholar]
  36. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  37. Zechman F. W. 2003; Phylogeny of the dasycladales (Chlorophyta, Ulvophyceae) based on analyses of RUBISCO large subunit (rbcL) gene sequences. J Phycol 39:819–827 [CrossRef]
    [Google Scholar]
  38. Zhang J., Huss V. A. R., Sun X., Chang K., Pang D. 2008; Morphology and phylogenetic position of a trebouxiophycean green alga (Chlorophyta) growing on the rubber tree, Hevea brasiliensis, with the description of a new genus and species. Eur J Phycol 43:185–193 [View Article]
    [Google Scholar]
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