1887

Abstract

Coccoid green algae are extremely diverse despite their simple coccoid phenotype, a phenotype that may be the result of convergent evolution. In this study, we used a polyphasic approach combining molecular phylogenetic analyses, morphology and ultrastructure to investigate isolated coccoid strains from China, and our results reveal three new lineages of Trebouxiophyceae: the novel genus and species gen. et sp. nov., and the two novel species sp. nov. and sp. nov. (Trebouxiophyceae, Chlorophyta). We provide a detailed characterization of the novel microalgae which they are autosporic coccoid unicells and have parietal chloroplasts. In phylogenies based on 18S rDNA sequences and the chloroplast ribulose-bisphosphate carboxylase gene (L), these three algae are nested within the clade and are different from any known algae. FACHB-1787 is not really close to any known algae within the clade. FACHB-2212 is within the lineages. FACHB-1793 is closely related to and described as a representative of a novel species of the genus .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001542
2016-12-01
2020-04-02
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5465.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001542&mimeType=html&fmt=ahah

References

  1. Aboal M., Werner O.. 2011; Morphology, fine structure, life cycle and phylogenetic analysis of Phyllosiphon arisari, a siphonous parasitic green alga. Eur J Phycol46:181–192 [CrossRef]
    [Google Scholar]
  2. Albertano P., Pollio A., Taddei R.. 1991; Viridiella fridericiana (Chlorococcales, Chlorophyta), a new genus and species isolated from extremely acid environments. Phycologia30:346–354 [CrossRef]
    [Google Scholar]
  3. Aslam Z., Shin W., Kim M. K., Im W. T., Lee S. T.. 2007; Marinichlorella kaistiae gen. et sp. nov. (Trebouxiophyceae, Chlorophyta) based on polyphasic taxonomy. J Phycol43:576–584[CrossRef]
    [Google Scholar]
  4. Bock C., Krienitz L., Proschold T.. 2011; Taxonomic reassessment of the genus Chlorella (Trebouxiophyceae) using molecular signatures (barcodes), including description of seven new species. Fottea11:293–312 [CrossRef]
    [Google Scholar]
  5. Darienko T., Gustavs L., Mudimu O., Menendez C. R.. 2010; Chloroidium, a common terrestrial coccoid green alga previously assigned to Chlorella (Trebouxiophyceae, Chlorophyta). Eur J Phycol45:79–95 [CrossRef]
    [Google Scholar]
  6. Fučíková K., Leliaert F., Cooper E. D., Å kaloud P., D'Hondt S., De Clerck O., Gurgel C. F. D., Lewis L. A., Lewis P. O. et al. 2014a; New phylogenetic hypotheses for the core chlorophyta based on chloroplast sequence data. Front Ecol Evol2:1–12
    [Google Scholar]
  7. Fučíková K., Lewis L. A., Lewis P. A.. 2014b; Widespread desert affiliation of trebouxiophycean algae (Trebouxiophyceae, Chlorophyta) including discovery of three new desert genera. Phycological Res62:294–305[CrossRef]
    [Google Scholar]
  8. Hallmann C., Stannek L., Fritzlar D., Hause-Reitner D., Friedl T., Hoppert M.. 2013; Molecular diversity of phototrophic biofilms on building stone. FEMS Microbiol Ecol84:355–372 [CrossRef][PubMed]
    [Google Scholar]
  9. Hanagata N., Karube I., Chihara M., Silva P. C.. 1998; Reconsideration of the taxonomy of ellipsoidal species of Chlorella (Trebouxiophyceae, Chlorophyta), with establishment of Watanabea sen. nov. Phycol Res46:221–229 [CrossRef]
    [Google Scholar]
  10. Karsten U., Friedl T., Schumann R., Hoyer K., Lembcke S.. 2005; Mycosporing-like amino acids and phylogenies in green algae: Prasiola and ITS relatives from the Trebouxiophyceae (Chlorophyta). J Phycol41:557–566[CrossRef]
    [Google Scholar]
  11. Katoh K., Kuma K., Toh H., Miyata T.. 2005; MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Res33:511–518 [CrossRef][PubMed]
    [Google Scholar]
  12. Krienitz L., Hegewald E. H., Hepperle D., Wolf M.. 2003; The systematics of coccoid green algae: 18S rRNA gene sequence data versus morphology. Biologia58:437–446
    [Google Scholar]
  13. Krienitz L., Huss V. A. R., Bock C.. 2015; Chlorella: 125 years of the green survivalist. Trends Plant Sci20:67–69 [CrossRef][PubMed]
    [Google Scholar]
  14. Kulichová J., Škaloud P., Neustupa J.. 2014; Molecular diversity of green corticolous microalgae from two sub-mediterranean European localities. Eur J Phycol49:345–355 [CrossRef]
    [Google Scholar]
  15. Kühn J.. 1878; über eine neue parasitische alge phyllosiphon arisari welche die Laubblätter einer terrestrischen Pflanze ganz in derselben Weise befällt, wie dies von parasitischen Pilzen bekanntist. Sitzungsber Naturf Ges Halle2:24–26
    [Google Scholar]
  16. Leliaert F., Smith D. R., Moreau H., Herron M. D., Verbruggen H., Delwiche C. F., De Clerck O.. 2012; Phylogeny and molecular evolution of the green algae. Crit Rev Plant Sci31:1–46[CrossRef]
    [Google Scholar]
  17. Lewin R. A., Krienitz L., Goericke R., Takeda H., Hepperle D.. 2000; Picocystis salinarum gen. et sp. nov. (Chlorophyta) – a new picoplanktonic green alga. Phycologia39:560–565 [CrossRef]
    [Google Scholar]
  18. Luo W., Pröschold T., Bock C., Krienitz L.. 2010; Generic concept in Chlorella-related coccoid green algae (Chlorophyta, Trebouxiophyceae). Plant Biol12:545–553 [CrossRef][PubMed]
    [Google Scholar]
  19. Ma S., Huss V. A. R., Tan D., Sun X., Chen J., Xie Y., Zhang J.. 2013; A novel species in the genus Heveochlorella (Trebouxiophyceae, Chlorophyta) witnesses the evolution from an epiphytic into an endophytic lifestyle in tree-dwelling green algae. Eur J Phycol48:200–209 [CrossRef]
    [Google Scholar]
  20. Medlin L., Elwood H. J., Stickel S., Sogin M. L.. 1988; The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene71:491–499 [CrossRef]
    [Google Scholar]
  21. Nadson G.. 1906; K morphologii nizshix vodoroslej [Zur Morphologie der niederen Algen.]. Izv Imp S-Peterburgsk Bot Sada6:184–194
    [Google Scholar]
  22. Neustupa J., Němcová Y., Eliáš M., Škaloud P.. 2009; Kalinella bambusicola gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel coccoid Chlorella-like subaerial alga from Southeast Asia. Phycological Res57:159–169 [CrossRef]
    [Google Scholar]
  23. Neustupa J., Eliáš M., Škaloud P., Němcová Y., Šejnohová L.. 2011; Xylochloris irregularis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga. Phycologia50:57–66 [CrossRef]
    [Google Scholar]
  24. Neustupa J., Nemcova Y., Vesela J., Steinova J., Skaloud P.. 2013a; Leptochlorella corticola gen. et sp. nov. and Kalinella apyrenoidosa sp. nov.: two novel Chlorella-like green microalgae (Trebouxiophyceae, Chlorophyta) from subaerial habitats. Int J Syst Evol Microbiol63:377–387 [CrossRef][PubMed]
    [Google Scholar]
  25. Neustupa J., Němcová Y., Veselá J., Steinová J., Škaloud P.. 2013b; Parachloroidium gen. nov. (Trebouxiophyceae, Chlorophyta), a novel genus of coccoid green algae from subaerial corticolous biofilms. Phycologia52:411–421 [CrossRef]
    [Google Scholar]
  26. Nyati S., Beck A., Honegger R.. 2007; Fine structure and phylogeny of green algal photobionts in the microfilamentous genus Psoroglaena (Verrucariaceae, lichen-forming ascomycetes). Plant Biol9:390–399 [CrossRef][PubMed]
    [Google Scholar]
  27. Nylander J. A. A.. 2004; MrModeltest 2.1 Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Uppsala Sweden:
    [Google Scholar]
  28. Němcová Y., Eliáš M., Škaloud P., Hodač L., Neustupa J.. 2011; Jenufa gen. nov. a new genus of coccoid green algae (Chlorophyceae, Incertae Sedis) previously recorded by environmental sequencing. J Phycol47:928–938[CrossRef]
    [Google Scholar]
  29. Posada D., Crandall K. A.. 1998; Modeltest: testing the model of DNA substitution. Bioinformatics14:817–818[CrossRef]
    [Google Scholar]
  30. Procházková K., Nmcová Y., Kulichová J., Neustupa J.. 2015; Morphology and phylogeny of parasitic and free-living members of the genus Phyllosiphon (Trebouxiophyceae, Chlorophyta). Nova Hedwigia101:501–518 [CrossRef]
    [Google Scholar]
  31. Reynolds C. S.. 2007; Variability in the provision and function of mucilage in phytoplankton: facultative responses to the environment. Hydrobiologia578:37–45 [CrossRef]
    [Google Scholar]
  32. Ronquist F., Huelsenbeck J. P.. 2003; MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics19:1572–1574 [CrossRef][PubMed]
    [Google Scholar]
  33. Song H., Zhang Q., Liu G., Hu Z.. 2015; Polulichloris henanensis gen. et sp. nov.(Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga. Phytotaxa218:137–146[CrossRef]
    [Google Scholar]
  34. Swofford D. L.. 2003; paup*. Phylogenetic analysis using parsimony (* and other methods) Version 4. Sinauer Associates; Sunderland, MA:
  35. Štifierová A., Neustupa J.. 2015; Community structure of corticolous microalgae within a single forest stand: evaluating the effects of bark surface pH and tree species. Fottea15:113–122[CrossRef]
    [Google Scholar]
  36. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  37. Zechman F. W.. 2003; Phylogeny of the dasycladales (Chlorophyta, Ulvophyceae) based on analyses of RUBISCO large subunit (rbcL) gene sequences. J Phycol39:819–827[CrossRef]
    [Google Scholar]
  38. Zhang J., Huss V. A. R., Sun X., Chang K., Pang D.. 2008; Morphology and phylogenetic position of a trebouxiophycean green alga (Chlorophyta) growing on the rubber tree, Hevea brasiliensis, with the description of a new genus and species. Eur J Phycol43:185–193 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001542
Loading
/content/journal/ijsem/10.1099/ijsem.0.001542
Loading

Data & Media loading...

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error