sp. nov., isolated from a tidal flat Free

Abstract

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated strain OITF-19, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-19 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain OITF-19 belonged to the genus , clustering with the type strain of , with which it shared 96.5 % sequence similarity. Strain OITF-19 exhibited the highest 16S rRNA gene sequence similarity to CAU 1002 (97.0 %) and of 92.0–96.8 % to the type strains of other species. Strain OITF-19 contained MK-7 as the predominant menaquinone and summed feature 3 (C 7 and/or C 6), iso-C and iso-C 3-OH as the major fatty acids. The major polar lipids detected in strain OITF-19 were phosphatidylcholine, phosphatidylethanolamine and three unidentified lipids. The DNA G+C content of strain OITF-19 was 38.3 mol%. Mean DNA–DNA relatedness between strain OITF-19 and the type strain of was 26 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain OITF-19 is separated from recognized species of the genus . On the basis of the data presented, strain OITF-19 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is OITF-19 (=KCTC 52456=NBRC 112418).

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2016-12-01
2024-03-28
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References

  1. Alegado R. A., Grabenstatter J. D., Zuzow R., Morris A., Huang S. Y., Summons R. E., King N. 2013; Algoriphagus machipongonensis sp. nov., co-isolated with a colonial choanoflagellate. Int J Syst Evol Microbiol 63:163–168 [View Article][PubMed]
    [Google Scholar]
  2. Barrow G. I, Feltham R. K. A. 1993 Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [CrossRef]
    [Google Scholar]
  3. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . In The Prokaryotes pp. 1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
    [Google Scholar]
  4. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  5. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868 [View Article][PubMed]
    [Google Scholar]
  6. Bowman J. P., Nichols C. M., Gibson J. A. 2003; Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53:1343–1355 [View Article][PubMed]
    [Google Scholar]
  7. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
    [Google Scholar]
  8. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol 49:25–68 [View Article]
    [Google Scholar]
  9. Copa-Patiño J. L., Arenas M., Soliveri J., Sánchez-Porro C., Ventosa A. 2008; Algoriphagus hitonicola sp. nov., isolated from an athalassohaline lagoon. Int J Syst Evol Microbiol 58:424–428 [View Article][PubMed]
    [Google Scholar]
  10. Embley T. M., Wait R. 1994; Structural lipids of eubacteria. In Modern Microbial Methods Chemical Methods in Prokaryotic Systematics pp. 121–161 Edited by Goodfellow M., O’Donnell A. G. Chichester: John Wiley & Sons;
    [Google Scholar]
  11. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  12. Jung Y.-T., Lee J.-S., Yoon J.-H. 2015; Algoriphagus aestuarii sp. nov., a member of the Cyclobacteriaceae isolated from a tidal-flat sediment of the Yellow Sea in Korea. Int J Syst Evol Microbiol 65:3439–3446 [View Article][PubMed]
    [Google Scholar]
  13. Kang H., Weerawongwiwat V., Jung M. Y., Myung S. C., Kim W. 2013; Algoriphagus chungangensis sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 63:648–653 [View Article][PubMed]
    [Google Scholar]
  14. Kim H., Joung Y., Joh K. 2014; Algoriphagus taeanensis sp. nov., isolated from a tidal flat, and emended description of Algoriphagus hitonicola . Int J Syst Evol Microbiol 64:21–26 [View Article][PubMed]
    [Google Scholar]
  15. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [CrossRef]
    [Google Scholar]
  16. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  17. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [CrossRef]
    [Google Scholar]
  18. Liu Y., Jiang J.-T., Liu Y.-H., Song X.-F., Xu C.-J., Liu Z.-P. 2009; Algoriphagus aquatilis sp. nov., isolated from a freshwater lake. Int J Syst Evol Microbiol 59:1759–1763 [View Article][PubMed]
    [Google Scholar]
  19. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  20. Nedashkovskaya O. I., Vancanneyt M., Van Trappen S., Vandemeulebroecke K., Lysenko A. M., Rohde M., Falsen E., Frolova G. M., Mikhailov V. V, Swings J. 2004; Description of Algoriphagus aquimarinus sp. nov., Algoriphagus chordae sp. nov. and Algoriphagus winogradskyi sp. nov., from seawater and algae, transfer of Hongiella halophila Yi & Chun 2004 to the genus Algoriphagus as Algoriphagus halophilus comb. nov. emended descriptions of the genera Algoriphagus Bowman et al. 2003 and Hongiella Yi and Chun 2004. Int J Syst Evol Microbiol 54:1757–1764 [CrossRef]
    [Google Scholar]
  21. Nedashkovskaya O. I., Kim S. B., Kwon K. K., Shin D. S., Luo X., Kim S. J., Mikhailov V. V. 2007; Proposal of Algoriphagus vanfongensis sp. nov., transfer of members of the genera Hongiella Yi and Chun 2004 emend. Nedashkovskaya et al. 2004 and Chimaereicella Tiago et al. 2006 to the genus Algoriphagus, and emended description of the genus Algoriphagus Bowman et al. 2003 emend. Nedashkovskaya et al. 2004. Int J Syst Evol Microbiol 57:1988–1994 [View Article][PubMed]
    [Google Scholar]
  22. Nedashkovskaya O. I., Ludwig W. 2011; Family II. Cyclobacteriaceae fam. nov. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 4 pp. 423–440 Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. Baltimore: Williams & Wilkins;
    [Google Scholar]
  23. Oh K.-H., Kang S.-J., Lee S.-Y., Park S., Oh T.-K., Yoon J.-H. 2012; Algoriphagus namhaensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 62:575–579 [View Article][PubMed]
    [Google Scholar]
  24. Parte A. C. 2014; LPSN-list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42:D613–D616 [View Article][PubMed]
    [Google Scholar]
  25. Park S., Kang S.-J., Oh K.-H., Oh T.-K., Yoon J.-H. 2010; Algoriphagus lutimaris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 60:200–204 [View Article][PubMed]
    [Google Scholar]
  26. Park S., Park J.-M., Lee K.-C., Yoon J.-H. 2014a; Algoriphagus boseongensis sp. nov., a member of the family Cyclobacteriaceae isolated from a tidal flat. Antonie van Leeuwenhoek 105:523–531 [View Article][PubMed]
    [Google Scholar]
  27. Park S., Won S.-M., Kim H., Park D.-S., Yoon J.-H. 2014b; Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 64:2969–2974 [View Article][PubMed]
    [Google Scholar]
  28. Park S., Kim S., Jung Y.-T., Yoon J.-H. 2016; Algoriphagus confluentis sp. nov., isolated from the junction between the ocean and a freshwater lake. Int J Syst Evol Microbiol 66:118–124 [View Article][PubMed]
    [Google Scholar]
  29. Reichenbach H. 1992; The order Cytophagales . In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn. pp. 3631–3675 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  30. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  31. Shahina M., Hameed A., Lin S.-Y., Lai W.-A., Hsu Y.-H., Young C.-C. 2014; Description of Algoriphagus taiwanensis sp. nov., a xylanolytic bacterium isolated from surface seawater, and emended descriptions of Algoriphagus mannitolivorans, Algoriphagus olei, Algoriphagus aquatilis and Algoriphagus ratkowskyi . Antonie van Leeuwenhoek 106:1031–1040 [View Article][PubMed]
    [Google Scholar]
  32. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNADNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 44:846–849 [View Article]
    [Google Scholar]
  33. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942[PubMed]
    [Google Scholar]
  34. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  35. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  36. Yang C., Li Y., Guo Q., Lai Q., Zheng T., Tian Y. 2013; Algoriphagus zhangzhouensis sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 63:1621–1626 [View Article][PubMed]
    [Google Scholar]
  37. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
    [Google Scholar]
  38. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter-and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
    [Google Scholar]
  39. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [View Article][PubMed]
    [Google Scholar]
  40. Yoon J.-H., Kang S.-J., Jung S.-Y., Lee C.-H., Oh T.-K. 2005; Algoriphagus yeomjeoni sp. nov., isolated from a marine solar saltern in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55:865–870 [View Article][PubMed]
    [Google Scholar]
  41. Yoon J.-H., Lee M.-H., Kang S.-J., Oh T.-K. 2006; Algoriphagus terrigena sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:777–780 [View Article][PubMed]
    [Google Scholar]
  42. Young C.-C., Lin S.-Y., Arun A. B., Shen F.-T., Chen W.-M., Rekha P. D., Langer S., Busse H.-J., Wu Y.-H., Kämpfer P. 2009; Algoriphagus olei sp. nov., isolated from oil-contaminated soil. Int J Syst Evol Microbiol 59:2909–2915 [View Article][PubMed]
    [Google Scholar]
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