@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001537, author = "Ma, Zhaoxu and Zhao, Shanshan and Cao, Tingting and Liu, Chongxi and Huang, Ying and Gao, Yuhang and Yan, Kai and Xiang, Wensheng and Wang, Xiangjing", title = "Verrucosispora sonchi sp. nov., a novel endophytic actinobacterium isolated from the leaves of common sowthistle (Sonchus oleraceus L.)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "12", pages = "5430-5436", doi = "https://doi.org/10.1099/ijsem.0.001537", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001537", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "16S rRNA gene", keywords = "Polyphasic taxonomy", keywords = "gyrB gene", keywords = "Sonchus oleraceus L", keywords = "Verrucosispora sonchi sp. nov.", abstract = "A novel actinobacterium, designated strain NEAU-QY3T, was isolated from the leaves of Sonchus oleraceus L. and examined using a polyphasic taxonomic approach. The organism formed single spores with smooth surface on substrate mycelia. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the strain had a close association with the genus Verrucosispora and shared the highest sequence similarity with Verrucosispora qiuiae RtIII47T (99.17 %), an association that was supported by a bootstrap value of 94 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. The strain also showed high 16S rRNA gene sequence similarities to Xiangella phaseoli NEAU-J5T (98.78 %), Jishengella endophytica 202201T (98.51 %), Micromonospora eburnea LK2-10T (98.28 %), Verrucosispora lutea YIM 013T (98.23 %) and Salinispora pacifica CNR-114T (98.23 %). Furthermore, phylogenetic analysis based on the gyrB gene sequences supported the conclusion that strain NEAU-QY3T should be assigned to the genus Verrucosispora . However, the DNA–DNA hybridization relatedness values between strain NEAU-QY3T and V. qiuiae RtIII47T and V. lutea YIM 013T were below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA–DNA hybridization results, strain NEAU-QY3T was readily distinguished from its most closely related strains and classified as a new species, for which the name Verrucosispora sonchi sp. nov. is proposed. The type strain is NEAU-QY3T (=CGMCC 4.7312T=DSM 101530T).", }