1887

Abstract

A novel Gram-stain-positive, non-motile, rod-shaped bacterial strain designated LIP-1 was isolated from the contaminated soil of a pesticide factory in Xinyi, China, was investigated for its taxonomic allocation by a polyphasic approach. Cell growth occurred at 16–37 °C (optimum, 30 °C), in the presence of 0–2.0 % (w/v) NaCl (optimum, 0 %) and at pH 6.0–9.0 (optimum, pH 7.0). The major fatty acids of strain LIP-1 were anteiso-C (50.8 %), iso-C (17.6 %) and anteiso-C (17.4 %). The cell-wall peptidoglycan type was B2δ with 2,4-diaminobutyric acid as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol and two unidentified glycolipids. The major menaquinones were MK-12 and MK-11. The genomic DNA G+C content was approximately 63.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain LIP-1 formed a distinct clade within the radiation of the family and had the highest sequence similarity with Gsoil 259 (96.01 %) followed by SK1 (94.94 %). On the basis of the phylogenetic analyses and distinct phenotypic characteristics, a new genus, namely gen. nov., is proposed, harbouring the novel species gen. nov., sp. nov. with the type strain LIP-1 (=CCTCC AB 2015422=KCTC 39698).

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2016-12-01
2020-01-21
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