1887

Abstract

A novel actinomycete strain, designated XHU 5089, was isolated from a hypersaline habitat in China. The strain was aerobic, Gram-stain-positive and the optimum NaCl concentration for growth was 1–3 % (w/v). Phylogenetic analysis based on the almost-complete 16S rRNA gene sequence of strain XHU 5089 showed that the organism was related most closely to TRM 49136 (97.7 % similarity). However, it had a relatively low mean DNA–DNA relatedness value with TRM 49136 (18.9±5.8 %). The whole-cell sugar pattern consisted of glucose and galactose. The predominant menaquinones were MK-10(H) and MK-9(H). The major fatty acids were anteiso-C, iso-C, iso-C and anteiso-C. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unknown phospholipids. The G+C content of the genomic DNA was 68.6 mol%. A novel species, sp. nov., is proposed, with XHU 5089 (=CCTCC AA 2015034=KCTC 39688) as the type strain.

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2016-12-01
2020-03-29
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References

  1. Cerny G.. 1978; Studies on the aminopeptidase test for the distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol5:113–122 [CrossRef]
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  3. Evtushenko L. I, Taptykova S. D., Akimov V. N., Semyonova S. A., Felsenstein J.. 1991; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E.. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39:224–229 [CrossRef]
    [Google Scholar]
  5. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–789 [CrossRef]
    [Google Scholar]
  7. Fitch W. M.. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool20:406–416 [CrossRef]
    [Google Scholar]
  8. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N.. 1974; Nocardia coeliaca, Nocardia autotrophica, and the Nocardin Strain. Int J Syst Bacteriol24:54–63 [CrossRef]
    [Google Scholar]
  9. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K.. 1997; Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol47:1129–1133 [CrossRef][PubMed]
    [Google Scholar]
  10. Gu Q., Zheng W., Huang Y.. 2007; Glycomyces sambucus sp. nov., an endophytic actinomycete isolated from the stem of Sambucus adnata Wall. Int J Syst Evol Microbiol57:1995–1998 [CrossRef][PubMed]
    [Google Scholar]
  11. Guan T. W., Xiao J., Zhao K., Luo X. X., Zhang X. P., Zhang L. L.. 2010; Halomonas xinjiangensis sp. nov., a halotolerant bacterium isolated from a salt lake. Int J Syst Evol Microbiol60:349–352 [CrossRef][PubMed]
    [Google Scholar]
  12. Guan T. W., Xia Z. F., Xiao J., Wu N., Chen Z. J., Zhang L. L., Zhang X. P.. 2011; Glycomyces halotolerans sp. nov., a novel actinomycete isolated from a hypersaline habitat in Xinjiang, China. Antonie v an Leeuwenhoek 100:137–143 [CrossRef][PubMed]
    [Google Scholar]
  13. Han X. X., Luo X. X., Zhang L. L.. 2014; Glycomyces fuscus sp. nov. and Glycomyces albus sp. nov., actinomycetes isolated from a hypersaline habitat. Int J Syst Evol Microbiol64:2437–2441 [CrossRef][PubMed]
    [Google Scholar]
  14. He L., Li W., Huang Y., Wang L., Liu Z., Lanoot B., Vancanneyt M., Swings J.. 2005; Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol55:1939–1944 [CrossRef][PubMed]
    [Google Scholar]
  15. Kelly K. L.. 1964; Inter-Society Color Council-National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  16. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  17. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  18. Labeda D. P., Testa R. T., Lechevalier M. P., Lechevalier H. A.. 1985; Glycomyces, a new genus of the Actinomycetales. Int J Syst Bacteriol35:417–421[CrossRef]
    [Google Scholar]
  19. Labeda D. P., Kroppenstedt R. M.. 2004; Emended description of the genus Glycomyces and description of Glycomyces algeriensis sp. nov., Glycomyces arizonensis sp. nov. and Glycomyces lechevalierae sp. nov. Int J Syst Evol Microbiol54:2343–2346 [CrossRef][PubMed]
    [Google Scholar]
  20. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L.. 2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol57:1424–1428 [CrossRef][PubMed]
    [Google Scholar]
  21. Lv L. L., Zhang Y. F., Zhang L. L.. 2015; Glycomyces tarimensis sp. nov., an actinomycete isolated from a saline-alkali habitat. Int J Syst Evol Microbiol65:1587–1591 [CrossRef][PubMed]
    [Google Scholar]
  22. Mesbah M., Premachandran U., Whitman W. B.. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39:159–167 [CrossRef]
    [Google Scholar]
  23. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241 [CrossRef]
    [Google Scholar]
  24. Qin S., Wang H. B., Chen H. H., Zhang Y. Q., Jiang C. L., Xu L. H., Li W. J.. 2008; Glycomyces endophyticus sp. nov., an endophytic actinomycete isolated from the root of Carex baccans Nees. Int J Syst Evol Microbiol58:2525–2528 [CrossRef][PubMed]
    [Google Scholar]
  25. Qin S., Chen H. H., Klenk H. P., Zhao G. Z., Li J., Xu L. H., Li W. J.. 2009; Glycomyces scopariae sp. nov. and Glycomyces mayteni sp. nov., isolated from medicinal plants in China. Int J Syst Evol Microbiol59:1023–1027 [CrossRef][PubMed]
    [Google Scholar]
  26. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  27. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newwark, DE: MIDI Inc;
    [Google Scholar]
  28. Shirling E. B., Gottlieb D.. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol16:313–340 [CrossRef]
    [Google Scholar]
  29. Staneck J. L., Roberts G. D.. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol28:226–231[PubMed]
    [Google Scholar]
  30. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  31. Tang S. K., Wang Y., Chen Y., Lou K., Cao L. L., Xu L. H., Li W. J.. 2009; Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. Int J Syst Evol Microbiol59:2025–2031 [CrossRef][PubMed]
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I, Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37:463–464[CrossRef]
    [Google Scholar]
  33. Williams S. T.. 1967; Sensitivity of streptomycetes to antibiotics as a taxonomic character. J Gen Microbiol46:151–160 [CrossRef][PubMed]
    [Google Scholar]
  34. Xing K., Qin S., Zhang W. D., Cao C. L., Ruan J. S., Huang Y., Jiang J. H.. 2014; Glycomyces phytohabitans sp. nov., a novel endophytic actinomycete isolated from the coastal halophyte in Jiangsu, East China. J Antibiot67:559–563 [CrossRef][PubMed]
    [Google Scholar]
  35. Zhang X., Ren K., Du J., Liu H., Zhang L.. 2014; Glycomyces artemisiae sp. nov., an endophytic actinomycete isolated from the roots of Artemisia argyi. Int J Syst Evol Microbiol64:3492–3495 [CrossRef][PubMed]
    [Google Scholar]
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