1887

Abstract

Recently, it has been proposed that strains of from the type III genetic division should be classified as subsp. subsp. nov., with strains from the type I and II divisions collectively classified as subsp. subsp. nov. Under such a taxonomic re-appraisal, we believe that types I and II should also have their own separate rank of subspecies. In support of this, we describe a polyphasic taxonomic study based on the analysis of publicly available multilocus and whole-genome sequence datasets, alongside a systematic review of previously published phylogenetic, genomic, phenotypic and clinical data. Strains of types I and II form highly distinct clades on the basis of multilocus sequence analysis (MLSA) and whole-genome phylogenetic reconstructions. or digital DNA–DNA similarity values also fall within the 70–80 % boundary recommended for bacterial subspecies. Furthermore, we see important differences in genome content, including the presence of an active CRISPR/Cas system in type II strains, but not type I, and evidence for increasing linkage equilibrium within the separate divisions. Key biochemical differences include positive test results for β-haemolytic, neuraminidase and sorbitol fermentation activities with type I strains, but not type II. We now propose that type I strains should be classified as subsp. subsp. nov., and type II as subsp. subsp. nov. The type strain of subsp. subsp. nov. is NCTC 737 (=ATCC 6919=JCM 6425=DSM 1897=CCUG 1794), while the type strain of subsp. subsp. nov. is ATCC 11828 (=JCM 6473=CCUG 6369).

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2016-12-01
2020-01-28
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