1887

Abstract

Ginseng-cultivated soil is an excellent habitat for soil-borne bacteria to proliferate. A novel strain, DCY87, was isolated from ginseng-cultivated soil in Gochang County, Republic of Korea, and subsequently characterized by polyphasic approach. Cells were rod shaped, non-motile, aerobic, Gram-reaction-positive, oxidase-negative and catalase-positive. 16S rRNA gene sequence analysis showed that strain DCY87 shared the highest similarity to ‘’ L1b-b9 (98.7 %). Closely phylogenetic relatives of strain DCY87 were identified: BXN5-13 (97.9 %), THG-a14 (97.8 %), MSL-03 (97.3 %), V2M29 (97.3 %), 5516T-20 (97.3 %), DS-8 (97.3 %) and KSW2-15 (97.1 %). The major polar lipids were classified as phosphatidylinositol and diphosphatidylglycerol. The major cellular fatty acids were composed of iso-C, anteiso-C, C and C 8. The menaquinone was resolved as MK-8(H). Strain DCY87 contained -diaminopimelic acid as diamino acid in the cell-wall peptidoglycan and glucose, xylose and rhamnose in the whole-cell sugar. The genomic DNA G+C content was calculated to be 72.7 mol%. DNA–DNA hybridization value between strain DCY87 and ‘’ L1b-b9 was estimated to be 50 %. However, DNA–DNA hybridization value obtained between strain DCY87 and BXN5-13, THG-a14 and MSL-03 was well below 17 %. In general, polyphasic taxonomy demonstrated that DCY87 strain represented a novel species within the genus . Accordingly, we propose the name sp. nov. The type strain is DCY87 (=KCTC 39635=JCM 31016).

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2016-12-01
2020-04-07
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