1887

Abstract

A Gram-stain-positive, strictly aerobic, mesophilic bacterium, designated H004, was isolated from a water sample of the hypersaline and heliothermal Lake Ursu, Sovata, Romania. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H004 formed a distinct phyletic lineage within the genus . It shared the highest 16S rRNA gene sequence similarity with YIM 70056 (98.80 %), followed by LMG 3623 (98.73 %), YIM 65003 (98.73 %), C5 (98.58 %) and DS472 (98.53 %). The -based phylogenetic analysis further confirmed that this strain constitutes a highly unique lineage within the genus. Chemotaxonomic characteristics, including the predominant fatty acids acids C, Cω9, Cω11/C ω9 and Cω7/iso-C 2-OH, the major quinone MK-8(H), the presence of mycolic acids and cell-wall chemotype IV were also consistent with the properties of members of the genus . The DNA G+C content of strain H004 was 65.4 mol%. The results of DNA–DNA hybridization analyses with the closest relatives, in combination with the based phylogenetic analysis, as well as the chemotaxonomic and physiological data, demonstrated that isolate H004 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H004 (=DSM 102881=NCAIM B.02632).

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2017-02-01
2020-01-25
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