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Abstract

A Gram-stain-positive, rod-shaped bacterium (strain JZ16) was isolated from raw cow's milk from the bulk tank of a dairy farm in Germany. The 16S rRNA gene sequence of the isolate showed a similarity of 98.3 % to the nearest related type strain ATCC 13032, a similarity of 97.6 % to GIMN1.010 and a similarity of 97.4 % to DSM 20147. Determination of chemotaxonomic characteristics revealed oleic acid (18 : 1 9) as the predominant fatty acid, major amounts of hexadecanoic acid (16 : 0) and minor amounts of heptadecanoic acid (17 : 0). The isolate showed an acetyl type of peptidoglycan and corynemycolic acids. The menaquinones MK-8(H) and MK-9(H) and the phospholipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected, which was in agreement with the description of the genus . Strain JZ16 was positive for reduction of nitrate to nitrite, pyrazinamidase, β-glucuronidase, β-glucosidase and urease activities. Acid was produced from -glucose, -ribose and -mannitol, but not from -xylose, maltose, lactose, sucrose and glycogen. The results of phylogenetic, phenotypic and chemotaxonomic analyses enabled the differentiation of the isolated strain from other closely related species of the genus . Therefore, strain JZ16 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JZ16 (=DSM 100882=CCUG 69192=LMG 29813).

Keyword(s): Corynebacterium and raw milk
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2016-12-01
2021-07-29
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