1887

Abstract

Three actinobacterial strains, FXJ8.128, FXJ8.269 and FXJ8.309, were isolated from deep-sea sediments collected from the Carlsberg Ridge and Southwest Indian Ridge at depths of 3690, 1800 and 2461 m, respectively. The three strains had highly similar 16S rRNA gene sequences (99.8–99.9 % identities) and formed a monophyletic clade within the Brevibacterium 16S rRNA gene tree, showing 98.2–98.9 % 16S rRNA gene sequence identities with type strains Brevibacterium epidermidis NCIMB 702286, Brevibacterium iodinum DSM 20626, Brevibacterium linens DSM 20425, Brevibacterium oceani BBH7 and Brevibacterium permense VKM Ac-2280. All three isolates showed activity towards the breakdown of pectin and fluoranthene. They contained MK-8(H2) as the most predominant menaquinone, diphosphatidylglycerol, phosphatidylglycerol and a glycolipd as the main polar lipids, and anteiso-C15 : 0 and anteiso-C17 : 0 as the major cellular fatty acids. Moreover, the three isolates were distinguished readily from the phylogenetically related type strains by DNA–DNA hybridization values, by random amplified polymorphic DNA fingerprint profiles and by a range of physiological and biochemical characteristics. On the basis of the above polyphasic taxonomic data, strains FXJ8.128, FXJ8.269 and FXJ8.309 represent a novel species of the genus Brevibacterium , for which the name Brevibacterium sediminis sp. nov. is proposed. The type strain is FXJ8.269 (=CGMCC 1.15472=DSM 102229).

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2016-12-01
2019-10-22
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