Frigoribacterium salinisoli sp. nov., isolated from saline soil, transfer of Frigoribacterium mesophilum to Parafrigoribacterium gen. nov. as Parafrigoribacterium mesophilum comb. nov.
A Gram-stain-positive, short-rod, aerobic bacterium, designated as strain LAM9155T, was isolated from saline soil sample collected from Lingxian County, Shandong Province, PR China. The strain grew optimally at 25–30 °C, pH 7.0 and 0.5 % (w/v) NaCl. The 16S rRNA gene sequence analysis revealed that strain LAM9155T belonged to the genus Frigoribacterium and was closely related to Frigoribacteriumendophyticum EGI 6500707T (99.4 %), Frigoribacteriumfaeni 801T (98.6 %) and Frigoribacteriummesophilum MSL-08T (96.2 %). The DNA–DNA hybridization values between strain LAM9155T and F. endophyticum JCM 30093T and between strain LAM9155T and F. faeni DSM 10309T were 40.2±2.1 and 32.8±1.6 %, respectively. The major fatty acids of LAM9155T were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The cell-wall analysis showed the B-type peptidoglycan containing alanine, glutamate, glycine, serine and lysine and that the cell wall contained the sugars galactose and ribose. The genomic DNA G+C content of strain LAM9155T was 68.2 mol%. The predominant menaquinone was MK-9. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, one unknown glycolipid and four unknown lipids. Based on the DNA–DNA hybridization and phenotypic, phylogenetic and chemotaxonomic properties, strain LAM9155T could be distinguished from the recognized species of the genus Frigoribacterium and was suggested to represent a novel species, for which the name Frigoribacterium salinisoli sp. nov. is proposed. The type strain is LAM9155T (=ACCC 19902T=JCM 30848T). Moreover, the transfer of F. mesophilum Dastager et al. 2008 to Parafrigoribacterium gen. nov. as Parafrigoribacterium mesophilum comb. nov. (type strain MSL-08T=DSM 19442T=KCTC 19311T) is also proposed.
De LeyJ.,
CattoirH.,
ReynaertsR.1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142[PubMed][CrossRef]
KämpferP.,
RaineyF. A.,
AnderssonM. A.,
Nurmiaho LassilaE. L.,
UlrychU.,
BusseH. J.,
WeissN.,
MikkolaR.,
Salkinoja-SalonenM.2000; Frigoribacterium faeni gen. nov., sp. nov., a novel psychrophilic genus of the family Microbacteriaceae
. Int J Syst Evol Microbiol 50:355–363 [View Article][PubMed]
KellyK. L.1964; Color-name charts illustrated with centroid colors. In Inter-Society Color Council-National Bureau of Standards Chicago: United States National Bureau of Standards;
KimS. J.,
JangY. H.,
HamadaM.,
TamuraT.,
AhnJ. H.,
WeonH. Y.,
SuzukiK.,
KwonS. W.2012b; Homoserinimonas aerilata gen. nov., sp. nov., a novel member of the family Microbacteriaceae isolated from an air sample in Korea. J Microbiol 50:673–679 [View Article][PubMed]
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
LiH. R.,
YuY.,
LuoW.,
ZengY. X.2010; Marisediminicola antarctica gen. nov., sp. nov., an actinobacterium isolated from the Antarctic. Int J Syst Evol Microbiol 60:2535–2539 [View Article][PubMed]
MarmurJ.,
DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
MinnikinD. E.,
O'DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
SchumannP.,
ZhangD. C.,
RedzicM.,
MargesinR.2012; Alpinimonas psychrophila gen. nov., sp. nov., an actinobacterium of the family Microbacteriaceae isolated from alpine glacier cryoconite. Int J Syst Evol Microbiol 62:2724–2730 [View Article][PubMed]
SmibertR. M.,
KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
StackebrandtE.,
GoebelB. M.1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 44:846–849 [View Article]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
WangH. F.,
ZhangY. G.,
ChenJ. Y.,
GuoJ. W.,
LiL.,
HozzeinW. N.,
ZhangY. M.,
WadaanM. A.,
LiW. J.2015; Frigoribacterium endophyticum sp. nov., an endophytic actinobacterium isolated from the root of Anabasis elatior (C. A. Mey.) Schischk. Int J Syst Evol Microbiol 65:1207–1212 [View Article][PubMed]
XuX. W.,
HuoY. Y.,
WangC. S.,
OrenA.,
CuiH. L.,
VedlerE.,
WuM.2011; Pelagibacterium halotolerans gen. nov., sp. nov. and Pelagibacterium luteolum sp. nov., novel members of the family Hyphomicrobiaceae
. Int J Syst Evol Microbiol 61:1817–1822 [View Article][PubMed]
Frigoribacterium salinisoli sp. nov., isolated from saline soil, transfer of Frigoribacterium mesophilum to Parafrigoribacterium gen. nov. as Parafrigoribacterium mesophilum comb. nov.