1887

International Journal of Systematic and Evolutionary Microbiology

Volume 66, Issue 12

Description of ‘ Phytoplasma meliae’, a phytoplasma associated with Chinaberry (.) yellowing in South America Free

Abstract

China tree yellows (ChTY) phytoplasma is associated with the yellowing disease of the China tree () in Argentina. According to partial 16S rRNA gene analysis, ChTY phytoplasma belongs to the 16Sr XIII group, subgroup G. Strains of species of ChTY have 98–99 % 16S rDNA gene sequence similarity with 16SrXIII-group phytoplasmas, and less than 97.5 % when compared to all ‘ ’ described so far, except for the novel '. However, strains of species of ChTY are differentiated from the latter due to having additional molecular and biological attributes. The presence of unique features in the 16S rDNA sequence distinguishes ChTY from all species of ‘ ’ currently described. The i RFLP profile of 16S rDNA (1.2 kb) and rpLV-rpsC (1.3 kb) genes distinguished ChTY, as in the 16SrXIII-G subgroup within the 16SrXIIII group. The phylogenetic analyses, based on 16S rDNA, rpLV-rpsC and secA gene sequences, in addition to the restricted host range, characteristic symptoms and geographical distribution, confirm that the collective strains of the species ChTY represent a distinct lineage within the phytoplasma clade and support the description of a novel species of ‘ ’ with the reference strain being ChTY-Mo3 (Montecarlo, Argentina).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rDNA , description , Melia azedarach , phytoplasma and yellowing
© 2016 IUMS
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2016-12-01
2021-10-16
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References

  1. Al-Saady N. A., Khan A. J., Calari A., Al-Subhi A. M., Bertaccini A. 2008; ‘ Candidatus Phytoplasma omanense', associated with witches'-broom of Cassia italica (Mill.) Spreng. in Oman. Int J Syst Evol Microbiol 58:461–466 [View Article][PubMed]
     [Google Scholar]
  2. Arneodo J. D., Galdeano E., Orrego A., Stauffer A., Nome S. F., Conci L. R. 2005; Identification of two phytoplasmas detected in China-trees with decline symptoms in Paraguay. Australas Plant Pathol 34:583–585 [View Article]
     [Google Scholar]
  3. Arneodo J. D., Marini D. C., Galdeano E., Meneguzzi N., Bacci M., Domecq C., Nome S. F., Conci L. R. 2007; Diversity and geographical distribution of phytoplasmas infecting China-tree in argentina. J Phytopathol 155:70–75 [View Article]
     [Google Scholar]
  4. Arocha Y., López M., Piñol B., Fernández M., Picornell B., Almeida R., Palenzuela I., Wilson M. R., Jones P. 2005; ‘ Candidatus Phytoplasma graminis' and ‘Candidatus Phytoplasma caricae', two novel phytoplasmas associated with diseases of sugarcane, weeds and papaya in Cuba. Int J Syst Evol Microbiol 55:2451–2463 [View Article][PubMed]
     [Google Scholar]
  5. Arocha Y., Antesana O., Montellano E., Franco P., Plata G., Jones P. 2007; ‘ Candidatus Phytoplasma lycopersici', a phytoplasma associated with ‘hoja de perejil' disease in Bolivia. Int J Syst Evol Microbiol 57:1704–1710 [View Article][PubMed]
     [Google Scholar]
  6. Ascher K. R. S., Schmutterer H., Zebitz C. P. W., Naqvi S. N. H. 1995; The Persian lilac or chinaberry tree: Melia azedarach L. In The Neem Tree – Source of Unique Natural Products in Integrated Pest Management, Medicine, Industry and Other Purposes pp. 605–642 Edited by Schmutterer H. Weinheim, Germany: VCH;
     [Google Scholar]
  7. Bertaccini A. 2007; Phytoplasmas: diversity, taxonomy, and epidemiology. Front Biosci 12:673–689 [View Article][PubMed]
     [Google Scholar]
  8. Bertaccini A., Duduk B., Paltrinieri S., Contaldo N. 2014; Phytoplasmas and phytoplasma diseases: a severe threat to agriculture. Am J Plant Sci 5:1763–1788 [View Article]
     [Google Scholar]
  9. Darriba D., Taboada G. L., Doallo R., Posada D. 2012; jModelTest 2: more models, new heuristics and parallel computing. Nat Methods 9:772 [View Article][PubMed]
     [Google Scholar]
  10. Davis R. E., Dally E. L., Gundersen D. E., Lee I. M., Habili N. 1997; ‘Candidatus phytoplasma australiense’ a new phytoplasma taxon associated with Australian grapevine yellows. Int J Syst Bacteriol 47:262–269 [View Article][PubMed]
     [Google Scholar]
  11. Davis R. E., Zhao Y., Dally E. L., Jomantiene R., Lee I. M., Wei W., Kitajima E. W. 2012; ‘ Candidatus Phytoplasma sudamericanum', a novel taxon, and strain PassWB-Br4, a new subgroup 16SrIII-V phytoplasma, from diseased passion fruit (Passiflora edulis f. flavicarpa Deg.). Int J Syst Evol Microbiol 62:984–989 [View Article][PubMed]
     [Google Scholar]
  12. Davis R. E., Zhao Y., Dally E. L., Lee I. M., Jomantiene R., Douglas S. M. 2013; ‘ Candidatus Phytoplasma pruni', a novel taxon associated with X-disease of stone fruits, Prunus spp.: multilocus characterization based on 16S rRNA, secY, and ribosomal protein genes. Int J Syst Evol Microbiol 63:766–776 [View Article][PubMed]
     [Google Scholar]
  13. Davis R. E., Harrison N. A., Zhao Y., Wei W., Dally E. L. 2016; ‘ Candidatus Phytoplasma hispanicum', a novel taxon associated with Mexican periwinkle virescence disease of Catharanthus roseus . Int J Syst Evol Microbiol 66:3463–3467 [CrossRef]
     [Google Scholar]
  14. Deng S., Hiruki C. 1991; Amplification of 16S rRNA genes from culturable and nonculturable mollicutes. J Microbiol Methods 14:53–61 [View Article]
     [Google Scholar]
  15. Doyle J., Doyle J. L. 1990; Isolation of plant DNA from fresh tissue. Focus 12:13–15
     [Google Scholar]
  16. Duarte V., Silva E. G., Hass I. C. R., Bedendo I. P., Kitajima E. W. 2009; First report of a group 16SrIII-B phytoplasma associated with decline of China tree in Brazil. Plant Disease 93:666 [View Article]
     [Google Scholar]
  17. Galdeano E., Torres L. E., Meneguzzi N., Guzman F., Gomez G. G., Docampo D. M., Conci L. R. 2004; Molecular characterization of 16S ribosomal DNA and phylogenetic analysis of two X-disease group phytoplasmas affecting China-tree (Melia azedarach L.) and garlic (Allium sativum L.) in Argentina. J Phytopathol 152:174–181 [View Article]
     [Google Scholar]
  18. Griffiths H. M., Sinclair W. A., Smart C. D., Davis R. E. 1999; The phytoplasma associated with ash yellows and lilac witches'-broom: ‘Candidatus phytoplasma fraxini'. Int J Syst Bacteriol 49:1605–1614 [View Article][PubMed]
     [Google Scholar]
  19. Guindon S., Dufayard J. F., Lefort V., Anisimova M., Hordijk W., Gascuel O. 2010; New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59:307–321 [View Article][PubMed]
     [Google Scholar]
  20. Han S.-S., Baek S.-J., Lee S.-H., Seo S.-T., Seralathan K.-K. 2015; First report of aster yellows phytoplasma (16SrI-B) associated with witches' broom disease of Melia azedarach var. japonica in Korea. J Phytopathol 163:1055–1058 [View Article]
     [Google Scholar]
  21. Harrison N. A., Boa E., Carpio M. L. 2003; Characterization of phytoplasmas detected in Chinaberry trees with symptoms of leaf yellowing and decline in Bolivia. Plant Pathol 52:147–157 [View Article]
     [Google Scholar]
  22. Harrison N. A., Carpio M. L., Boa E. 2006; First report of ‘Candidatus phytoplasma asteris’-related strains infecting Chinaberry trees with leaf yellowing symptoms in Vietnam. Plant Dis 90:527 [View Article]
     [Google Scholar]
  23. Harrison N. A., Davis R. E., Oropeza C., Helmick E. E., Narváez M., Eden-Green S., Dollet M., Dickinson M. 2014; ‘ Candidatus Phytoplasma palmicola', associated with a lethal yellowing-type disease of coconut (Cocos nucifera L.) in Mozambique. Int J Syst Evol Microbiol 64:1890–1899 [View Article][PubMed]
     [Google Scholar]
  24. Hiruki C., Wang K. 2004; Clover proliferation phytoplasma: ‘Candidatus Phytoplasma trifolii'. Int J Syst Evol Microbiol 54:1349–1353 [View Article][PubMed]
     [Google Scholar]
  25. Hogenhout S. A., Oshima K., Ammar D., Kakizawa S., Kingdom H. N., Namba S. 2008; Phytoplasmas: bacteria that manipulate plants and insects. Mol Plant Pathol 9:403–423 [View Article][PubMed]
     [Google Scholar]
  26. IRPCM 2004; ‘ Candidatus Phytoplasma', a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects. Int J Syst Evol Microbiol 54:1243–1255 [View Article][PubMed]
     [Google Scholar]
  27. Jung H. Y., Sawayanagi T., Kakizawa S., Nishigawa H., Miyata S., Oshima K., Ugaki M., Lee J. T., Hibi T. et al. 2002; ‘ Candidatus Phytoplasma castaneae', a novel phytoplasma taxon associated with chestnut witches' broom disease. Int J Syst Evol Microbiol 52:1543–1549 [View Article][PubMed]
     [Google Scholar]
  28. Jung H. Y., Sawayanagi T., Wongkaew P., Kakizawa S., Nishigawa H., Wei W., Oshima K., Miyata S., Ugaki M. et al. 2003a; ‘ Candidatus Phytoplasma oryzae', a novel phytoplasma taxon associated with rice yellow dwarf disease. Int J Syst Evol Microbiol 53:1925–1929 [View Article][PubMed]
     [Google Scholar]
  29. Jung H. Y., Sawayanagi T., Kakizawa S., Nishigawa H., Wei W., Oshima K., Miyata S., Ugaki M., Hibi T. et al. 2003b; ‘ Candidatus phytoplasma ziziphi', a novel phytoplasma taxon associated with jujube witches'-broom disease. Int J Syst Evol Microbiol 53:1037–1041 [View Article][PubMed]
     [Google Scholar]
  30. Lee I.-M., Hammond R. W., Davis R. E., Gundersen D. E. 1993; Universal amplification and analysis of pathogen 16S rDNA for classification and identification of MLOs. Phytopathology 83:834–842 [CrossRef]
     [Google Scholar]
  31. Lee I.-M., Gundersen-Rindal D. E., Davis R. E., Bartoszyk I. M. 1998; Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int J Syst Bacteriol 48:1153–1169 [View Article]
     [Google Scholar]
  32. Lee I. M., Davis R. E., Gundersen-Rindal D. E. 2000; Phytoplasma: phytopathogenic mollicutes. Annu Rev Microbiol 54:221–255 [View Article][PubMed]
     [Google Scholar]
  33. Lee I. M., Bottner K. D., Secor G., Rivera-Varas V. 2006; ‘Candidatus Phytoplasma americanum’, a phytoplasma associated with a potato purple top wilt disease complex. Int J Syst Evol Microbiol 56:1593–1597 [View Article][PubMed]
     [Google Scholar]
  34. Lee I. M., Bottner-Parker K. D., Zhao Y., Villalobos W., Moreira L. 2011; ‘ Candidatus Phytoplasma costaricanum' a novel phytoplasma associated with an emerging disease in soybean (Glycine max) . Int J Syst Evol Microbiol 61:2822–2826 [View Article][PubMed]
     [Google Scholar]
  35. Lee I.-M., Gundersen-Rindal D. E., Davis R. E., Bottner K. D., Marcone C., Seemüller E. 2004a; ‘ Candidatus Phytoplasma asteris', a novel phytoplasma taxon associated with aster yellows and related diseases. Int J Syst Bacteriol 54:1037–1048 [View Article]
     [Google Scholar]
  36. Lee I.-M., Martini M., Marcone C., Zhu S. F. 2004b; Classification of phytoplasma strains in the elm yellows group (16SrV) and proposal of ‘Candidatus Phytoplasma ulmi' for the phytoplasma associated with elm yellows. Int J Syst Bacteriol 54:337–347 [View Article]
     [Google Scholar]
  37. Lim P. O., Sears B. B. 1992; Evolutionary relationships of a plant-pathogenic mycoplasmalike organism and Acholeplasma laidlawii deduced from two ribosomal protein gene sequences. J Bacteriol 174:2606–2611[PubMed] [CrossRef]
     [Google Scholar]
  38. Malembic-Maher S., Salar P., Filippin L., Carle P., Angelini E., Foissac X. 2011; Genetic diversity of European phytoplasmas of the 16SrV taxonomic group and proposal of ‘Candidatus Phytoplasma rubi'. Int J Syst Evol Microbiol 61:2129–2134 [View Article][PubMed]
     [Google Scholar]
  39. Marcone C., Gibb K. S., Streten C., Schneider B. 2004a; ‘ Candidatus Phytoplasma spartii', ‘Candidatus Phytoplasma rhamni' and ‘Candidatus Phytoplasma allocasuarinae', respectively associated with spartium witches'-broom, buckthorn witches'-broom and allocasuarina yellows diseases. Int J Syst Evol Microbiol 54:1025–1029 [View Article]
     [Google Scholar]
  40. Marcone C., Schneider B., Seemüller E. 2004b; ‘ Candidatus Phytoplasma cynodontis', the phytoplasma associated with bermuda grass white leaf disease. Int J Syst Evol Microbiol 54:1077–1082 [View Article]
     [Google Scholar]
  41. Martini M., Marcone C., Mitrović J., Maixner M., Delić D., Myrta A., Ermacora P., Bertaccini A., Duduk B. 2012; ‘ Candidatus Phytoplasma convolvuli', a new phytoplasma taxon associated with bindweed yellows in four European countries. Int J Syst Evol Microbiol 62:2910–2915 [View Article][PubMed]
     [Google Scholar]
  42. Matsuoka K., Brune A., Ansani A., Carvalho M. G. 1986; Association of a mycoplasma-like organism with Melia azedarach in Paraguay. Revista Arvore 10:202–206
     [Google Scholar]
  43. Montano H. G., Davis R. E., Dally E. L., Hogenhout S., Pimentel J. P., Brioso P. S. 2001; ‘ Candidatus Phytoplasma brasiliense', a new phytoplasma taxon associated with hibiscus witches' broom disease. Int J Syst Evol Microbiol 51:1109–1118 [View Article][PubMed]
     [Google Scholar]
  44. Muñoz J. O., Nome S. F., Kitajima E. W. 1987; A mycoplasma like organism associated with yellows of Melia azedarach . Fitopatol Bras 12:95–97
     [Google Scholar]
  45. Nejat N., Vadamalai G., Davis R. E., Harrison N. A., Sijam K., Dickinson M., Abdullah S. N., Zhao Y. 2013; ‘ Candidatus Phytoplasma malaysianum', a novel taxon associated with virescence and phyllody of Madagascar periwinkle (Catharanthus roseus). Int J Syst Evol Microbiol 63:540–548 [View Article][PubMed]
     [Google Scholar]
  46. Pérez-López E., Luna-Rodríguez M., Olivier C. Y., Dumonceaux T. J. 2016; The underestimated diversity of phytoplasmas in Latin America. Int J Syst Evol Microbiol 66:492–513 [View Article][PubMed]
     [Google Scholar]
  47. Quaglino F., Zhao Y., Casati P., Bulgari D., Bianco P. A., Wei W., Davis R. E. 2013; ‘ Candidatus Phytoplasma solani', a novel taxon associated with stolbur- and bois noir-related diseases of plants. Int J Syst Evol Microbiol 63:2879–2894 [View Article][PubMed]
     [Google Scholar]
  48. Qui B. S., Li H. H., Sui C. L., Jiu K. X 1998; Amplification of phytoplasma 16SrDNA from 20 infected plants in China and their RFLP analysis. Sci Silvae Sin 34:67–74
     [Google Scholar]
  49. Ragonese A. E. 1981; Cultivo y mejoramiento del paraíso gigante (Melia azedarach var. gigantea cv. Garrasíno), en la República Argentina. Anales Academia Nacional De Agronomía y Veterinaria XXXV:5–10
     [Google Scholar]
  50. Rupa B. A., Azam N. K., Mannan A., Ahmed N., Hasan N. 2014; Phytochemistry and pharmacological appraisals of persian lilac (Melia azedarach Linn): a quick comprehensive review. Am J Ethnomed 1:152–163
     [Google Scholar]
  51. Šafářová D., Zemánek T., Válová P., Navrátil M. 2016; ‘ Candidatus Phytoplasma cirsii', a novel taxon from creeping thistle [Cirsiumarvense (L.) Scop]. Int J Syst Evol Microbiol 66:1745–1753 [View Article][PubMed]
     [Google Scholar]
  52. Sanna G., Madeddu S., Giliberti G., Ntalli N. G., Cottiglia F., De Logu A., Agus E., Caboni P. 2015; Limonoids from Melia azedarach fruits as inhibitors of flaviviruses and Mycobacterium tubercolosis . PLoS One 10:e0141272 [View Article][PubMed]
     [Google Scholar]
  53. Sawayanagi T., Horikoshi N., Kanehira T., Shinohara M., Bertaccini A., Cousin M. T., Hiruki C., Namba S. 1999; ‘ Candidatus phytoplasma japonicum’, a new phytoplasma taxon associated with Japanese hydrangea phyllody. Int J Syst Bacteriol 49:1275–1285 [CrossRef]
     [Google Scholar]
  54. Schneider B., Seemüller E., Smart C. D., Kirkpatrick B. C. 1995; Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas. In Molecular and Diagnostic Procedures in Mycoplasmology vol. 1 pp. 369–380 Edited by Razin S., Tully J. G. San Diego: Academic Press; [CrossRef]
     [Google Scholar]
  55. Schneider B., Torres E., Martín M. P., Schröder M., Behnke H. D., Seemüller E. 2005; ‘ Candidatus Phytoplasma pini', a novel taxon from Pinus silvestris and Pinus halepensis . Int J Syst Evol Microbiol 55:303–307 [View Article][PubMed]
     [Google Scholar]
  56. Seemüller E., Schneider B. 2004; ‘ Candidatus Phytoplasma mali', ‘Candidatus Phytoplasma pyri' and ‘Candidatus Phytoplasma prunorum', the causal agents of apple proliferation, pear decline and European stone fruit yellows, respectively. Int J Syst Evol Microbiol 54:1217–1226 [View Article][PubMed]
     [Google Scholar]
  57. Staden R., Beal K. F., Bonfield J. K. 2000; The staden package, 1998. Methods Mol Biol 132:115–130[PubMed]
     [Google Scholar]
  58. Valiunas D., Staniulis J., Davis R. E. 2006; ‘ Candidatus Phytoplasma fragariae', a novel phytoplasma taxon discovered in yellows diseased strawberry, Fragaria x ananassa. Int J Syst Evol Microbiol 56:277–281 [View Article][PubMed]
     [Google Scholar]
  59. Vázquez A., Ducasse D. A., Nome S. F., Muñoz J. O. 1983; Declinamiento del paraíso (Melia azedarach L.), síntomas y estudios etiológicos de esta nueva enfermedad. Revista de Investigaciones Agropecuarias – INTA 18:309–320
     [Google Scholar]
  60. Verdin E., Salar P., Danet J.-L., Choueiri E., Jreijiri F., El Zammar S., Gélie B., Bové J. M., Garnier M. 2003; ‘ Candidatus Phytoplasma phoenicium' sp. nov., a novel phytoplasma associated with an emerging lethal disease of almond trees in Lebanon and Iran. IntJ Syst Evol Microbiol 53:833–838 [View Article]
     [Google Scholar]
  61. Wei W., Davis R. E., Lee I. M., Zhao Y. 2007; Computer-simulated RFLP analysis of 16S rRNA genes: identification of ten new phytoplasma groups. Int J Syst Evol Microbiol 57:1855–1867 [View Article][PubMed]
     [Google Scholar]
  62. Wei W., Davis R. E., Jomantiene R., Zhao Y. 2008; Ancient, recurrent phage attacks and recombination shaped dynamic sequence-variable mosaics at the root of phytoplasma genome evolution. Proc Natl Acad Sci U S A 105:11827–11832 [View Article][PubMed]
     [Google Scholar]
  63. White D. T., Blackall L. L., Scott P. T., Walsh K. B. 1998; Phylogenetic positions of phytoplasmas associated with dieback, yellow crinkle and mosaic diseases of papaya, and their proposed inclusion in ‘Candidatus Phytoplasma australiense' and a new taxon, ‘Candidatus Phytoplasma australasia'. Int J Syst Bacteriol 48:941–951 [View Article][PubMed]
     [Google Scholar]
  64. Win N. K., Lee S. Y., Bertaccini A., Namba S., Jung H. Y. 2013; ‘ Candidatus Phytoplasma balanitae' associated with witches' broom disease of Balanites triflora . Int J Syst Evol Microbiol 63:636–640 [View Article][PubMed]
     [Google Scholar]
  65. Zhao Y., Wei W., Lee I.-M., Shao J., Suo X., Davis R. E. 2009a; Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). Int J Syst Evol Microbiol 59:2582–2593 [View Article]
     [Google Scholar]
  66. Zhao Y., Sun Q., Wei W., Davis R. E., Wu W., Liu Q. 2009b; ‘ Candidatus Phytoplasma tamaricis', a novel taxon discovered in witches'-broom-diseased salt cedar (Tamarix chinensis Lour.). Int J Syst Evol Microbiol 59:2496–2504 [View Article]
     [Google Scholar]
  67. Zreik L., Carle P., Bové J. M., Garnier M. 1995; Characterization of the mycoplasmalike organism associated with witches'-broom disease of lime and proposition of a Candidatus taxon for the organism, ‘Candidatus phytoplasma aurantifolia’. Int J Syst Bacteriol 45:449–453 [View Article][PubMed]
     [Google Scholar]
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Description of ‘Candidatus Phytoplasma meliae’, a phytoplasma associated with Chinaberry (Melia azedarach L.) yellowing in South America
Int J Syst Evol Microbiol 66, 5244 (2016); https://doi.org/10.1099/ijsem.0.001503
/content/journal/ijsem/10.1099/ijsem.0.001503
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