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Abstract

A Gram-stain-negative, rod-shaped strain, Braz8, isolated from larvae of was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Braz8 was related most closely to species of the genus , with 95.6, 96.5 and 96.6 % similarity to the type strains of , and , respectively, and formed a separate branch in the phylogenetic tree next to the monophyletic cluster of the genus . Chemotaxonomic data supported the allocation of the strain to the family . The major fatty acids were C , C and C. The quinone system was composed of ubiquinones Q-8 and Q-7 (1 : 0.3), the predominant polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the polyamine pattern showed the major compound putrescine. However, qualitative and quantitative differences in the major polyamine, polar lipid profile and fatty acid patterns distinguished strain Braz8 from species of the genus . Phylogenetic analysis based on 16S rRNA gene sequences, average nucleotide identity, DNA–DNA hybridization, multilocus sequence analysis as well as physiological and biochemical tests distinguished strain Braz8 both genotypically and phenotypically from the three species but also showed its placement in the family . Thus, strain Braz8 is considered to represent a novel species of a new genus most closely related to the genus , for which the name gen. nov., sp. nov. is proposed. The type strain of is Braz8 (=LMG 29552=CIP 111088).

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2016-12-01
2022-01-16
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