1887

Abstract

Five ascosporogenous yeast strains related to the genus were isolated. Two strains (CLIB 1764 and CLIB 1780) were isolated from French sourdoughs; three others (UFMG-CM-Y273, UFMG-CM-Y451 and UFMG-CM-Y452) were from rotting wood in Brazil. The sequences of the French and Brazilian strains differed by one and three substitutions, respectively, in the D1/D2 large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS). The D1/D2 LSU rRNA sequence of these strains differed by 0.5 and 0.7 % from , but their ITS sequences diverged by 8.1 and 8.3 %, respectively, from that of the closest described species . Analysis of protein coding sequences of , and distinguished the French from the Brazilian strains, with respectively 3.3, 6 and 11.7 % substitutions. Two novel species are described to accommodate these newly isolated strains: sp. nov. (type strain CLIB 1764=CBS 14374) and sp. nov. (type strain UFMG-CM-Y273=CLIB 1783=CBS 14236). Further analysis of culture collections revealed a strain previously assigned to the species, but having 3.8 % difference (22 substitutions and 2 indels) in its ITS with respect to . Hence, we describe a new taxon, sp. nov. (type strain CLIB 162=CBS 2141), to accommodate this strain. Finally, and are reassigned to the genus as new combinations. On the basis of sequence analysis, we also propose that and comb. nov. are conspecific.

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2016-12-01
2020-03-29
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