1887

Abstract

A study of the faecal microbiome in three healthy female rhesus macaques revealed the presence of a novel obligately anaerobic, chemoorganoheterotrophic, non-sporing, coccoid, non-motile, Gram-stain-positive bacterial species. Three strains of this species, designated as M108, M916-1/1, and M919-2/1, were non-haemolytic, H2S-positive, catalase-positive, bile- and NaCl-sensitive and required peptone for growth. Strains also were asaccharolytic, able to utilize sulfite, thiosulfate and elemental sulfur as electron acceptors, and produced acetic and butyric acids as metabolic end-products. Strain M108 is characterized by the prevalence of C14 : 0, C16 : 0 and C18 : 1ω9cis dimethyl acetal among the cellular fatty acids, and the presence of MK-10 menaquinone. The DNA G+C content was found to be 51 mol%. Phylogenetic analysis of partial 16S rRNA gene sequences of strains M108, M916-1/1 and M919-2/1 placed these strains into the genus Peptococcus (family Peptococcaceae ). On the basis of phenotypic and genotypic properties we conclude that these strains represent a novel bacterial species for which the name Peptococcus simiae sp. nov. is proposed. The type strain is M108 (=DSM 100347=VKM B-2932).

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2016-12-01
2019-10-21
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References

  1. Edgar R. C..( 2004;). muscle: multiple sequence alignment with high accuracy and high throughput. . Nucleic Acids Res32:1792–1797. [CrossRef][PubMed]
    [Google Scholar]
  2. Ezaki T..( 2009;). Family VI. Peptococcaceae. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn.,vol. 3, The Firmicutes , pp. 969–971. Edited by Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B.. New York:: Springer;.
    [Google Scholar]
  3. Felsenstein J..( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution39:783–791. [CrossRef]
    [Google Scholar]
  4. Hall I. C..( 1930;). Micrococcus niger, a new pigment forming anaerobic coccus recovered from urine in a case of general arteriosclerosis. . J Bacteriol20:407–415.
    [Google Scholar]
  5. Imachi H., Sakai S., Ohashi A., Harada H., Hanada S., Kamagata Y., Sekiguchi Y..( 2007;). Pelotomaculum propionicicum sp. nov., an anaerobic, mesophilic, obligately syntrophic, propionate-oxidizing bacterium. . Int J Syst Evol Microbiol57:1487–1492. [CrossRef][PubMed]
    [Google Scholar]
  6. Jabari L., Gannoun H., Cayol J. L., Hamdi M., Ollivier B., Fauque G., Fardeau M. L..( 2013;). Desulfotomaculum peckii sp. nov., a moderately thermophilic member of the genus Desulfotomaculum, isolated from an upflow anaerobic filter treating abattoir wastewaters. . Int J Syst Evol Microbiol63:2082–2087. [CrossRef][PubMed]
    [Google Scholar]
  7. Juteau P., Côté V., Duckett M. F., Beaudet R., Lépine F., Villemur R., Bisaillon J. G..( 2005;). Cryptanaerobacter phenolicus gen. nov., sp. nov., an anaerobe that transforms phenol into benzoate via 4-hydroxybenzoate. . Int J Syst Evol Microbiol55:245–250. [CrossRef][PubMed]
    [Google Scholar]
  8. Kluyver A. J., van Niel C. B..( 1936;). Prospects for a natural system of classification of bacteria. . Zentralbl Bakteriol Parasitenkd Infektionskr Abt2:369–403.
    [Google Scholar]
  9. Murdoch D. A., Mitchelmore I. J..( 1991;). The laboratory identification of Gram-positive anaerobic cocci. . J Med Microbiol34:295–308. [CrossRef][PubMed]
    [Google Scholar]
  10. Rogosa M..( 1971;). Peptococcaceae, a new family to include the Gram-positive, anaerobic cocci of the genera Peptococcus, Peptostreptococcus, and Ruminococcus. . Int J Syst Bacteriol21:234–237. [CrossRef]
    [Google Scholar]
  11. Sánchez-Andrea I., Stams A. J., Hedrich S., Nancucheo I., Johnson D. B..( 2015;). Desulfosporosinus acididurans sp. nov.: an acidophilic sulfate-reducing bacterium isolated from acidic sediments. . Extremophiles19:39–47. [CrossRef][PubMed]
    [Google Scholar]
  12. Sorokin D. Y., Muyzer G..( 2010;). Haloalkaliphilic spore-forming sulfidogens from soda lake sediments and description of Desulfitispora alkaliphila gen. nov., sp. nov. . Extremophiles14:313–320. [CrossRef][PubMed]
    [Google Scholar]
  13. Tamura K., Nei M..( 1993;). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. . Mol Biol Evol10:512–526.[PubMed]
    [Google Scholar]
  14. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol30:2725–2729. [CrossRef][PubMed]
    [Google Scholar]
  15. Turner S., Pryer K. M., Miao V. P., Palmer J. D..( 1999;). Investigating deep phylogenetic relationships among cyanobacteria and plastids by small subunit rRNA sequence analysis. . J Eukaryot Microbiol46:327–338. [CrossRef][PubMed]
    [Google Scholar]
  16. Van Eldere J. R., De Pauw G., Eyssen H. J..( 1987;). Steroid sulfatase activity in a Peptococcus niger strain from the human intestinal microflora. . Appl Environ Microbiol53:1655–1660.[PubMed]
    [Google Scholar]
  17. Wilkins T. D., Moore W. E. C., West S. E. H., Holdeman L. V..( 1975;). Peptococcus niger (Hall) kluyver and van Niel 1936: Emendation of description and designation of neotype strain. . Int J Syst Bacteriol25:47–49. [CrossRef]
    [Google Scholar]
  18. Zavarzina D. G., Sokolova T. G., Tourova T. P., Chernyh N. A., Kostrikina N. A., Bonch-Osmolovskaya E. A..( 2007;). Thermincola ferriacetica sp. nov., a new anaerobic, thermophilic, facultatively chemolithoautotrophic bacterium capable of dissimilatory Fe(III) reduction. . Extremophiles11:1–7. [CrossRef][PubMed]
    [Google Scholar]
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