1887

Abstract

A polyphasic taxonomic approach was applied to strains of the species in order to clarify the taxonomic legitimacy of the delineation of into , and A group of 81 isolates obtained from human (=62) and veterinary (=17) clinical materials and foods (=2) and ten reference and type strains obtained from the Czech Collection of Microorganisms were characterized by extensive biotyping using conventional tests and commercial identification kits (ID 32 Staph, STAPHYtest, Biolog Microbial ID System), matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry, automated ribotyping with RI restriction enzyme, 16S–23S rRNA gene intergenic transcribed spacer PCR fingerprinting and repetitive sequence-based PCR fingerprinting with the (GTG) primer. Selected strains representing different ribotypes were further characterized using sequencing of the β-subunit of RNA polymerase () gene. Individual techniques revealed high heterogeneity within the analysed strains but differentiation of the investigated strains into groups corresponding to the aforementioned subspecies and supported by these techniques was not clearly revealed. Based on obtained results and data retrieved from literature we propose rejecting the separation of species into , and and we suggest reclassification these subspecies as with the type strain W.E. Kloos SC 116 (=ATCC 29062=BCRC 12927=CCM 3473=CCUG 15598=CNCTC 5683=DSM 20345=JCM 2425=NCTC 12103).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001493
2016-12-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5181.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001493&mimeType=html&fmt=ahah

References

  1. Antignac A., Tomasz A. 2009; Reconstruction of the phenotypes of methicillin-resistant Staphylococcus aureus by replacement of the staphylococcal cassette chromosome mec with a plasmid-borne copy of Staphylococcus sciuri pbpD gene. Antimicrob Agents Chemother 53:435–441 [View Article][PubMed]
    [Google Scholar]
  2. Becker K., Von Eiff C. 2011; Staphylococcus, Micrococcus, and other catalase-positive cocci. In Manual of Clinical Microbiology, 10th edn. vol. 1 pp. 308–330 Edited by Versalovic J., Carroll K. C., Funke G., Jorgensen J. H., Landry M. L., Warnock D. W. Washington, D.C.: ASM Press; [CrossRef]
    [Google Scholar]
  3. Bergeron M., Dauwalder O., Gouy M., Freydiere A. M., Bes M., Meugnier H., Benito Y., Etienne J., Lina G. et al. 2011; Species identification of staphylococci by amplification and sequencing of the tuf gene compared to the gap gene and by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Eur J Clin Microbiol Infect Dis 30:343–354 [View Article][PubMed]
    [Google Scholar]
  4. Carretto E., Barbarini D., Couto I., De Vitis D., Marone P., Verhoef J., De Lencastre H., Brisse S. 2005; Identification of coagulase-negative staphylococci other than Staphylococcus epidermidis by automated ribotyping. Clin Microbiol Infect 11:177–184 [View Article][PubMed]
    [Google Scholar]
  5. Chesneau O., Morvan A., Aubert S., El Solh N. 2000; The value of rRNA gene restriction site polymorphism analysis for delineating taxa in the genus Staphylococcus . Int J Syst Evol Microbiol 50:689–697 [View Article][PubMed]
    [Google Scholar]
  6. Couto I., Pereira S., Miragaia M., Sanches I. S., De Lencastre H. 2001; Identification of clinical staphylococcal isolates from humans by internal transcribed spacer PCR. J Clin Microbiol 39:3099–3103 [View Article][PubMed]
    [Google Scholar]
  7. Dakić I., Morrison D., Vuković D., Savić B., Shittu A., Ježek P., Hauschild T., Stepanović S. 2005; Isolation and molecular characterization of Staphylococcus sciuri in the hospital environment. J Clin Microbiol 43:2782–2785 [CrossRef]
    [Google Scholar]
  8. Drancourt M., Raoult D. 2002; rpoB gene sequence-based identification of Staphylococcus species. J Clin Microbiol 40:1333–1338 [View Article][PubMed]
    [Google Scholar]
  9. Ghebremedhin B., Layer F., König W., König B. 2008; Genetic classification and distinguishing of Staphylococcus species based on different partial gap, 16S rRNA, hsp60, rpoB, sodA, and tuf gene sequences. J Clin Microbiol 46:1019–1025 [View Article][PubMed]
    [Google Scholar]
  10. Jensen M. A., Webster J. A., Straus N. 1993; Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Appl Environ Microbiol 59:945–952
    [Google Scholar]
  11. Kloos W. E., Schleifer K. H., Smith R. F. 1976; Characterization of Staphylococcus sciuri sp.nov. and its subspecies. Int J Syst Bacteriol 26:22–37 [View Article]
    [Google Scholar]
  12. Kloos W. E., Ballard D. N., Webster J. A., Hubner R. J., Tomasz A., Couto I., Sloan G. L., Dehart H. P., Fiedler F. et al. 1997; Ribotype delineation and description of Staphylococcus sciuri subspecies and their potential as reservoirs of methicillin resistance and staphylolytic enzyme genes. Int J Syst Bacteriol 47:313–323 [View Article][PubMed]
    [Google Scholar]
  13. Marsou R., Bes M., Boudouma M., Brun Y., Meugnier H., Freney J., Vandenesch F., Etienne J. 1999; Distribution of Staphylococcus sciuri subspecies among human clinical specimens, and profile of antibiotic resistance. Res Microbiol 150:531–541 [View Article][PubMed]
    [Google Scholar]
  14. Marsou R., Bes M., Brun Y., Boudouma M., Idrissi L., Meugnier H., Freney J., Etienne J. 2001; Molecular techniques open up new vistas for typing of coagulase-negative staphylococci. Pathol Biol 49:205–215 [View Article][PubMed]
    [Google Scholar]
  15. Mašlaňová I., Doškař J., Varga M., Kuntová L., Mužík J., Malúšková D., Růžičková V., Pantůček R. 2013; Bacteriophages of Staphylococcus aureus efficiently package various bacterial genes and mobile genetic elements including SCCmec with different frequencies. Env Microbiol Reports 5:66–73 [View Article][PubMed]
    [Google Scholar]
  16. Mellmann A., Becker K., von Eiff C., Keckevoet U., Schumann P., Harmsen D. 2006; Sequencing and staphylococci identification. Emerg Infect Dis 12:333–336 [View Article][PubMed]
    [Google Scholar]
  17. Nagase N., Sasaki A., Yamashita K., Shimizu A., Wakita Y., Kitai S., Kawano J. 2002; Isolation and species distribution of staphylococci from animal and human skin. J Vet Med Sci 64:245–250 [View Article][PubMed]
    [Google Scholar]
  18. Pantůček R., Švec P., Dajcs J. J., Machová I., Černohlávková J., Šedo O., Gelbíčová T., Mašlaňová I., Doškař J. et al. 2013; Staphylococcus petrasii sp. nov. including S. petrasii subsp. petrasii subsp. nov. and S. petrasii subsp. croceilyticus subsp. nov., isolated from human clinical specimens and human ear infections. Syst Appl Microbiol 36:90–95 [View Article][PubMed]
    [Google Scholar]
  19. Poyart C., Quesne G., Boumaila C., Trieu-Cuot P. 2001; Rapid and accurate species-level identification of coagulase-negative staphylococci by using the sodA gene as a target. J Clin Microbiol 39:4296–4301 [View Article][PubMed]
    [Google Scholar]
  20. Rolo J., De Lencastre H., Miragaia M. 2014; High frequency and diversity of cassette chromosome recombinases (ccr) in methicillin-susceptible Staphylococcus sciuri . J Antimicrob Chemother 69:1461–1469 [View Article][PubMed]
    [Google Scholar]
  21. Schleifer K. H., Bell J. A. 2009; Genus I. Staphylococcus Rosenbach 1884, 18AL (Nom. Cons. Opin. 17 Jud. Comm. 1958, 153.). In Bergey's Manual of Systematic Bacteriology, the Firmicutes vol. 3 pp. 392–421 Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Whitman W. B. New York: Springer;
    [Google Scholar]
  22. Schleifer K. H., Geyer U., Kilpper-Bälz R., Devriese L. A. 1983; Elevation of Staphylococcus sciuri subsp. lentus (Kloos et al.) to Species Status: Staphylococcus lentus (Kloos et al.) comb. nov. Syst Appl Microbiol 4:382–387 [View Article][PubMed]
    [Google Scholar]
  23. Shittu A., Lin J., Morrison D., Kolawole D. 2004; Isolation and molecular characterization of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus associated with skin and soft-tissue infections. J Med Microbiol 53:51–55 [View Article][PubMed]
    [Google Scholar]
  24. Stepanovi S., Ježek P., Vuković D., Daki I., Petráš P. 2003; Isolation of members of the Staphylococcus sciuri group from urine and their relationship to urinary tract infections. J Clin Microbiol 41:5262–5264 [View Article][PubMed]
    [Google Scholar]
  25. Stepanovi S., Daki I., Morrison D., Hauschild T., Ježek P., Petráš P., Martel A., Vukovi D., Shittu A. et al. 2005; Identification and characterization of clinical isolates of members of the Staphylococcus sciuri group. J Clin Microbiol 43:956–958 [View Article][PubMed]
    [Google Scholar]
  26. Švec P., Nováková D., Žáčková L., Kukletová M., Sedláček I. 2008; Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans . Anton Leeuw Int J G 94:573–579 [View Article][PubMed]
    [Google Scholar]
  27. Švec P., Pantůček R., Petráš P., Sedláček I., Nováková D. 2010; Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting. Syst Appl Microbiol 33:451–456 [View Article][PubMed]
    [Google Scholar]
  28. Švec P., De Bel A., Sedláček I., Petráš P., Gelbíčová T., Černohlávková J., Mašlaňová I., Cnockaert M., Varbanovová I. et al. 2015; Staphylococcus petrasii subsp. pragensis subsp. nov., occurring in human clinical material. Int J Syst Evol Microbiol 65:2071–2077 [View Article][PubMed]
    [Google Scholar]
  29. Wu S., De Lencastre H., Tomasz A. 1998; Genetic organization of the mecA region in methicillin-susceptible and methicillin-resistant strains of Staphylococcus sciuri . J Bacteriol 180:236–242[PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.001493
Loading
/content/journal/ijsem/10.1099/ijsem.0.001493
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error