1887

Abstract

A polyphasic taxonomic approach was applied to strains of the species in order to clarify the taxonomic legitimacy of the delineation of into , and A group of 81 isolates obtained from human (=62) and veterinary (=17) clinical materials and foods (=2) and ten reference and type strains obtained from the Czech Collection of Microorganisms were characterized by extensive biotyping using conventional tests and commercial identification kits (ID 32 Staph, STAPHYtest, Biolog Microbial ID System), matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry, automated ribotyping with RI restriction enzyme, 16S–23S rRNA gene intergenic transcribed spacer PCR fingerprinting and repetitive sequence-based PCR fingerprinting with the (GTG) primer. Selected strains representing different ribotypes were further characterized using sequencing of the β-subunit of RNA polymerase () gene. Individual techniques revealed high heterogeneity within the analysed strains but differentiation of the investigated strains into groups corresponding to the aforementioned subspecies and supported by these techniques was not clearly revealed. Based on obtained results and data retrieved from literature we propose rejecting the separation of species into , and and we suggest reclassification these subspecies as with the type strain W.E. Kloos SC 116 (=ATCC 29062=BCRC 12927=CCM 3473=CCUG 15598=CNCTC 5683=DSM 20345=JCM 2425=NCTC 12103).

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2016-12-01
2020-05-29
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