RT Journal Article SR Electronic(1) A1 Xu, Lin A1 Wu, Yue-Hong A1 Jian, Shu-Ling A1 Wang, Chun-Sheng A1 Wu, Min A1 Cheng, Lei A1 Xu, Xue-WeiYR 2016 T1 Pseudohongiellanitratireducens sp. nov., isolated from seawater, and emended description of the genus Pseudohongiella JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 12 SP 5155 OP 5160 DO https://doi.org/10.1099/ijsem.0.001489 PB Microbiology Society, SN 1466-5034, AB Two Gram-stain-negative, aerobic, motile by a single polar flagellum and rod-shaped strains, designated SCS-49T and SCS-111, were isolated from seawater of the South China Sea. The two strains grew at 4–35 °C, with 0.5–7.5 % (w/v) NaCl and at pH 6.5–9.0 and were able to reduce nitrate. Q-8 was the sole ubiquinone. The major fatty acids of the two strains were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c). The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospoglycolipid, three unidentified glycolipids, five unidentified phospholipids and two to three unidentified lipids. The isolates formed a stable clade with Pseudohongiella acticola and Pseudohongiella spirulinae based on phylogenetic analysis of 16S rRNA gene sequences. Strains SCS-49T and SCS-111 exhibited 16S rRNA gene sequence similarity values of 97.2 and 96.0 % with respect to the type strains of P. acticola and P. spirulinae , respectively. The average nucleotide diversity and in silico DNA–DNA hybridization values between strain SCS-49T and P. acticola KCTC 42131T were 71.4 and 25.1 %, respectively and the values between strain SCS-49T and SCS-111 were 99.9 and 99.2 %, respectively. Based upon the phenotypic, chemotaxonomic and genetic data, strains SCS-49T and SCS-111 represent a novel species in the genus Pseudohongiella , for which the name Pseudohongiella nitratireducens sp. nov. is proposed. The type strain is SCS-49T (=CGMCC 1.15425T=KCTC 52155T=MCCC 1K03186T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001489