1887

Abstract

The novel, pale yellow bacterial strain, designated S14-88, was isolated from a tundra soil near Antarctic Peninsula, South Shetland Islands, and its taxonomic position was investigated by a genotypic and phenotypic analysis. Cells were facultatively anaerobic, Gram-stain-negative, non-motile and rod-shaped. Growth occurred at 4–28 °C (optimum at 15 °C), at pH 7.0–8.0 (optimum at 7.0) and with 0–0.6 % (w/v) NaCl (optimum, no NaCl). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-88 formed a lineage within the genus . The 16S rRNA gene sequence similarity between strain S14-88 and the type strains of related species ranged from 92.2 to 96.5 %, and the 16S rRNA gene sequence of S14-88 showed highest similarity of 96.5 % to HME6664. The major cellular fatty acids of strain S14-88 were iso-C and summed feature 3 (C 7 and/or C 6). The major respiratory quinone was menaquinone MK-7, and the main polar lipid was phosphatidylethanolamine. The DNA G+C content of strain S14-88 was 42.3 mol%. On the basis of the evidence presented in this study, strain S14-88 is considered to represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is S14-88 (=CCTCC AB 2015321=KCTC 52232).

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2016-12-01
2020-01-17
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References

  1. Baik K. S., Park S. C., Kim E. M., Lim C. H., Seong C. N.. 2010; Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter. Int J Syst Evol Microbiol60:134–139 [CrossRef][PubMed]
    [Google Scholar]
  2. Barrow G. I., Feltham R. K. A.. (editors) 1993; Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press;[CrossRef]
    [Google Scholar]
  3. Bernardet J. F., Nakagawa Y., Holmes B.. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol52:1049–1070 [CrossRef][PubMed]
    [Google Scholar]
  4. Bowman J. P.. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol50:1861–1868 [CrossRef][PubMed]
    [Google Scholar]
  5. Chen X., Zhao R., Tian Y., Kong B., Li X., Chen Z., Li Y.. 2014; Mucilaginibacter polytrichastri sp. nov., isolated from a moss (Polytrichastrum formosum), and emended description of the genus Mucilaginibacter. Int J Syst Evol Microbiol64:1395–1400 [CrossRef][PubMed]
    [Google Scholar]
  6. Doetsch R. N.. 1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology pp.21–33 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Wood W. A., Krieg N. R., Phillips G. H.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  7. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–791 [CrossRef]
    [Google Scholar]
  9. Hwang Y. M., Baik K. S., Seong C. N.. 2014; Mucilaginibacter defluvii sp. nov., isolated from a dye wastewater treatment facility. Int J Syst Evol Microbiol64:565–571 [CrossRef][PubMed]
    [Google Scholar]
  10. Joung Y., Kim H., Kang H., Lee B., Ahn T. S., Joh K.. 2014a; Mucilaginibacter soyangensis sp. nov., isolated from a lake. Int J Syst Evol Microbiol64:413–419 [CrossRef][PubMed]
    [Google Scholar]
  11. Joung Y., Kim H., Lee B., Kang H., Kim T. S., Kim S. B., Joh K.. 2014b; Mucilaginibacter flavus sp. nov., isolated from wetland. Int J Syst Evol Microbiol64:1304–1309 [CrossRef][PubMed]
    [Google Scholar]
  12. Joung Y., Kang H., Lee B., Kim H., Joh K., Kim K.. 2015; Mucilaginibacter aquaedulcis sp. nov., isolated from fresh water. Int J Syst Evol Microbiol65:698–703 [CrossRef][PubMed]
    [Google Scholar]
  13. Kidd K. K., Zonta S.. 1971; Phylogenetic analysis: concepts and methods. Am J Hum Genet23:235–252
    [Google Scholar]
  14. Kim B. C., Lee K. H., Kim M. N., Lee J., Shin K. S.. 2010; Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum. FEMS Microbiol Lett309:130–135 [CrossRef][PubMed]
    [Google Scholar]
  15. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  16. Kämpfer P., Busse H. J., McInroy J. A., Glaeser S. P.. 2014; Mucilaginibacter auburnensis sp. nov., isolated from a plant stem. Int J Syst Evol Microbiol64:1736–1742 [CrossRef][PubMed]
    [Google Scholar]
  17. Lane D. J.. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp.115–147 Edited by Stackebrandt E., Goodfellow M.. Chichester: Wiley;
    [Google Scholar]
  18. Lee K. C., Kim K. K., Eom M. K., Kim J. S., Kim D. S., Ko S. H., Lee J. S.. 2015; Mucilaginibacter gotjawali sp. nov., isolated from soil of a lava forest. Int J Syst Evol Microbiol65:952–958 [CrossRef][PubMed]
    [Google Scholar]
  19. Luo X., Zhang L., Dai J., Liu M., Zhang K., An H., Fang C.. 2009; Mucilaginibacter ximonensis sp. nov., isolated from Tibetan soil. Int J Syst Evol Microbiol59:1447–1450 [CrossRef][PubMed]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B.. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39:159–167 [CrossRef]
    [Google Scholar]
  21. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241 [CrossRef]
    [Google Scholar]
  22. Montero-Calasanz M., Göker M., Rohde M., Spröer C., Schumann P., Busse H. J., Schmid M., Tindall B. J., Klenk H. P., Camacho M.. 2013; Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery and emendation of the species Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium. Int J Syst Evol Microbiol63:4386–4395 [CrossRef][PubMed]
    [Google Scholar]
  23. Paiva G., Abreu P., Proença D. N., Santos S., Nobre M. F., Morais P. V.. 2014; Mucilaginibacter pineti sp. nov., isolated from Pinus pinaster wood from a mixed grove of pines trees. Int J Syst Evol Microbiol64:2223–2228 [CrossRef][PubMed]
    [Google Scholar]
  24. Pankratov T. A., Tindall B. J., Liesack W., Dedysh S. N.. 2007; Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. Int J Syst Evol Microbiol57:2349–2354 [CrossRef][PubMed]
    [Google Scholar]
  25. Park C. S., Han K., Ahn T. Y.. 2014; Mucilaginibacter koreensis sp. nov., isolated from leaf mould. Int J Syst Evol Microbiol64:2274–2279 [CrossRef][PubMed]
    [Google Scholar]
  26. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  27. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
    [Google Scholar]
  28. Tindall B. J.. 1990; Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett66:199–202 [CrossRef]
    [Google Scholar]
  29. Urai M., Aizawa T., Nakagawa Y., Nakajima M., Sunairi M.. 2008; Mucilaginibacter kameinonensis sp., nov., isolated from garden soil. Int J Syst Evol Microbiol58:2046–2050 [CrossRef][PubMed]
    [Google Scholar]
  30. Xie C. H., Yokota A.. 2003; Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol49:345–349 [CrossRef][PubMed]
    [Google Scholar]
  31. Yumoto I., Hirota K., Nodasaka Y., Yokota Y., Hoshino T., Nakajima K.. 2004; Alkalibacterium psychrotolerans sp. nov., a psychrotolerant obligate alkaliphile that reduces an indigo dye. Int J Syst Evol Microbiol54:2379–2383 [CrossRef][PubMed]
    [Google Scholar]
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