RT Journal Article SR Electronic(1) A1 Kämpfer, Peter A1 Busse, Hans-Jürgen A1 Kleinhagauer, Tanita A1 McInroy, John A. A1 Glaeser, Stefanie P.YR 2016 T1 Saccharibacillus endophyticus sp. nov., an endophyte of cotton JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 12 SP 5134 OP 5139 DO https://doi.org/10.1099/ijsem.0.001484 PB Microbiology Society, SN 1466-5034, AB A Gram-positive-staining, facultatively aerobic, endospore-forming bacterial strain, isolated from the stem tissue of cotton (Gossypium hirsutum) was subjected to a detailed taxonomic study using a polyphasic approach. Based on 16S rRNA gene sequence similarity comparisons, strain JM-1350T grouped into the genus Saccharibacillus , and was most closely related to the type strain of Saccharibacillus sacchari (96.5 % 16S rRNA gene sequence similarity), followed by the type strains of Saccharibacillus kuerlensis (96.3 %) and Saccharibacillus deserti (95.1 %). The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The quinone system contained exclusively menaquinone MK-7. The polar lipid (L1) profile consisted of the major lipids diphosphatidylglycerol and phosphatidylglycerol and moderate amounts of three glycolipids and an aminophospholipid and a polar lipid (L1). The major fatty acids were iso- and anteiso-branched fatty acids. The genomic G+C content was 55.2 mol%. In addition, the results of physiological and biochemical tests allowed phenotypic differentiation of strain JM-1350T from all closely related species. Thus, strain JM-1350T represents a novel species of the genus Saccharibacillus , for which the name Saccharibacillus endophyticus sp. nov. is proposed. The type strain is JM-1350T (=LMG 29710T=CCM 8702T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001484