1887

Abstract

A Gram-stain-positive, aerobic, non-motile, coccoid bacterial strain, 36A, was isolated from Munnar, in India. Based on the results of 16S rRNA gene sequence analysis, this strain was identified as representing a member of the genus Tersicoccus and was most closely related to the type strain of Tersicoccus phoenicis (98.9 %, sequence similarity), the only other member of the genus, and to members of the related genus Arthrobacter (<96.1 %, sequence similarity). However, the DNA–DNA relatedness of strain 36A with T. phoenicis DSM 30849 was only 31.9±0.8. The DNA G+C content of strain 36A was 70.9 mol%. Strain 36A was an aerobic microbe with optimal growth at 37 °C, pH 6.0–8.0 and NaCl 0.5-3 % (w/v). Cell-wall peptidoglycan of strain 36A was of type A11.20 (Lys–Ser–Ala2). Polar lipids present were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, two phospholipids, a glycolipid and four unknown lipids. The major isoprenoid quinones were MK-9 (H2) and MK-8 (H2). Major fatty acids of strain 36A were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0. The results of phylogenetic, chemotaxonomic and biochemical tests allowed a clear differentiation of strain 36A, which represents a novel member of the genus Tersicoccus , for which the name Tersicoccus solisilvae sp. nov., is proposed. The type strain is 36A (=KCTC 33776=CGMCC 1.15480).

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2016-12-01
2019-12-05
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