1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 12

sp., nov., a bacterium isolated from forest soil Free

Abstract

A Gram-stain-positive, aerobic, non-motile, coccoid bacterial strain, 36A, was isolated from Munnar, in India. Based on the results of 16S rRNA gene sequence analysis, this strain was identified as representing a member of the genus and was most closely related to the type strain of (98.9 %, sequence similarity), the only other member of the genus, and to members of the related genus (<96.1 %, sequence similarity). However, the DNA–DNA relatedness of strain 36A with DSM 30849 was only 31.9±0.8. The DNA G+C content of strain 36A was 70.9 mol%. Strain 36A was an aerobic microbe with optimal growth at 37 °C, pH 6.0–8.0 and NaCl 0.5-3 % (w/v). Cell-wall peptidoglycan of strain 36A was of type A11.20 (Lys–Ser–Ala). Polar lipids present were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, two phospholipids, a glycolipid and four unknown lipids. The major isoprenoid quinones were MK-9 (H) and MK-8 (H). Major fatty acids of strain 36A were anteiso-C, anteiso-C, iso-C and iso-C. The results of phylogenetic, chemotaxonomic and biochemical tests allowed a clear differentiation of strain 36A, which represents a novel member of the genus , for which the name sp. nov., is proposed. The type strain is 36A (=KCTC 33776=CGMCC 1.15480).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): forest soil , India , polyphasic taxonomy , Tersicoccus solisilvae sp., nov., and Western Ghats
© 2016 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001470
2016-12-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5061.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001470&mimeType=html&fmt=ahah

References

  1. Hasegawa T., Takizaea M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [View Article]
     [Google Scholar]
  2. Kates M. 1972; Techniques of Lipidology. pp 330–342 New York: Elsevier;
  3. Kates M. 1986; Techniques of lipidology: isolation, analysis, and identification of lipids. pp 330–348 Amsterdam: Elsevier;
  4. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  5. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
     [Google Scholar]
  6. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. et al. 2007; ‘clustal w and clustal x version 2.0’. Bioinformatics 23:2947–2948 [View Article]
     [Google Scholar]
  7. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
     [Google Scholar]
  8. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
     [Google Scholar]
  9. Meier-Kolthoff J. P., Göker M., Spröer C., Klenk H.-P. 2013; When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 195:413–418 [View Article][PubMed]
     [Google Scholar]
  10. Oren A., Duker S., Ritter S. 1996; The polar lipid composition of Walsby's square bacterium. FEMS Microbiol Lett 138:135–140 [View Article]
     [Google Scholar]
  11. Parte A. C. 2016; List of prokaryotic names with standing in nomenclature. http://www.bacterio.net
  12. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI Inc;
  13. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156 [CrossRef]
     [Google Scholar]
  14. Schumann P. 2011; Peptidoglycan Structure. In Taxonomy of Prokaryotes, Methods in Microbiology vol. 38 pp. 101–129 Edited by Rainey F., Oren A. London: Academic Press; [CrossRef]
     [Google Scholar]
  15. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477[PubMed]
     [Google Scholar]
  16. Smibert R. M., Krieg N. R. 1981; General characterization. In Manual of Methods for General Microbiology pp. 409–443 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  17. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  18. Stackebrandt E., Goebel B. M. 1994; Place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
     [Google Scholar]
  19. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 4:152–155
     [Google Scholar]
  20. Tamaoka J., Katayama-Fujimura Y., Kuraishi H. 1983; Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Microbiol 54:31–36 [View Article]
     [Google Scholar]
  21. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  22. Tindall B. J. 1990a; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
     [Google Scholar]
  23. Tindall B. J. 1990b; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
     [Google Scholar]
  24. Tourova T. P., Antonov A. S. 1987; Identification of microorganisms by rapid DNA–DNA hybridization. Meth Microbiol 19:333–355 [CrossRef]
     [Google Scholar]
  25. Vaishampayan P., Moissl-Eichinger C., Pukall R., Schumann P., Spröer C., Augustus A., Roberts A. H., Namba G., Cisneros J. et al. 2013; Description of Tersicoccus phoenicis gen. nov., sp. nov. isolated from spacecraft assembly clean room environments. Int J Syst Evol Microbiol 63:2463–2471 [View Article][PubMed]
     [Google Scholar]
  26. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1982; Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128:1959–1968
     [Google Scholar]
  27. Vishnuvardhan Reddy S., Aspana S., Tushar D. L., Sasikala C. H., Ramana V. 2013; Spirochaeta sphaeroplastigenens sp. nov., a halo-alkaliphilic, obligately anaerobic spirochaete isolated from soda lake Lonar. Int J Syst Evol Microbiol 63:2223–2228 [View Article]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001470
Loading
Tersicoccus solisilvae sp., nov., a bacterium isolated from forest soil
Int J Syst Evol Microbiol 66, 5061 (2016); https://doi.org/10.1099/ijsem.0.001470
/content/journal/ijsem/10.1099/ijsem.0.001470
/content/journal/ijsem/10.1099/ijsem.0.001470
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error