1887

Abstract

A novel actinobacterial strain, designated SD, was isolated from rhizosphere soil of Chinese cabbage in Shandong province, China. The cells were aerobic, Gram-staining-positive, oxidase- and catalase-positive, short rods and formed white colonies on trypticase soy agar. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid and alanine, glutamic acid and leucine. Diphosphatidylglycerol was the predominant polar lipid. The predominant cellular fatty acid was C18 : 1 ω9c; minor components were anteiso-C15 : 0 and anteiso-C17 : 0. The only isoprenoid quinone was demethylmenaquinone 7 (DMK-7), and the DNA G+C content was 72.7 mol%. Based on the full-length 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of strain SD were Patulibacter medicamentivorans DSM 25962 (96.9 %), Patulibacter minatonensis DSM 18081 (96.7 %), Patulibacter americanus DSM 16676 (96.2 %) and Patulibacter ginsengiterrae DSM 25990 (95.9 %). Considering combined phenotypic and genotypic characteristics, it is proposed that strain SD represents a novel species of the genus Patulibacter , for which the name Patulibacter brassicae sp. nov. is proposed. The type strain is SD (=CICC 24108=KCTC 39817).

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2016-12-01
2019-10-15
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