1887

Abstract

A novel actinomycete strain, designated TRM 46515, was isolated from a salt water beach at Awat, Xinjiang, Northwest China, and characterized using polyphasic taxonomy. Comparison of 16S rRNA gene sequences showed that strain TRM 46515 is a member of the genus Streptomyces , exhibiting highest similarity with Streptomyces qinglanensis 172205 (98.32 %). However, DNA–DNA relatedness and phenotypic data readily distinguished strain TRM 46515 from phylogenetically related type strains. The G+C content of the DNA was 70.40 mol%. Whole-cell hydrolysates of strain TRM 46515 were found to contain ll-diaminopimelic acid as the diagnostic diamino acid and ribose was the major whole-cell sugar. The major fatty acids identified were anteiso-C17 : 0, anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0. The main menaquinone was MK-9(H8) and the polar lipids were identified as diphosphatidylglycerol, phosphatidyl ethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and one unknown glycolipid. On the basis of these phenotypic, chemotaxonomic and phylogenetic data, strain TRM 46515 should be designated as a representative of a novel species of the genus Streptomyces , for which the name Streptomyces litoralis sp. nov. is proposed. The type strain is TRM 46515 (=CCTCC AA 2015040=KCTC 39729).

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2016-12-01
2019-10-18
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References

  1. Chun J., Goodfellow M..( 1995;). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. . Int J Syst Bacteriol45:240–245. [CrossRef][PubMed]
    [Google Scholar]
  2. Collins C. H., Lyne P. M., Grange J. M., Falkinham J. O..( 2004;). Microbiological Methods, , 8th edn.. London:: Arnold;.
    [Google Scholar]
  3. Collins M. D., Jones D..( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. . J Appl Bacteriol48:459–470. [CrossRef]
    [Google Scholar]
  4. Collins M. D..( 1985;). Isoprenoid quinone analysis in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E.. London:: Academic Press;.
    [Google Scholar]
  5. Cui Y., Baek S. H., Wang L., Lee H. G., Cui C., Lee S. T., Im W. T..( 2012;). Streptomyces panacagri sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol62:780–785. [CrossRef][PubMed]
    [Google Scholar]
  6. Edgar R. C..( 2004;). muscle: multiple sequence alignment with high accuracy and high throughput. . Nucleic Acids Res32:1792–1797. [CrossRef][PubMed]
    [Google Scholar]
  7. Felsenstein J..( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol17:368–376. [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J..( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution39:783–789. [CrossRef]
    [Google Scholar]
  9. Fitch W. M..( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool20:406–416. [CrossRef]
    [Google Scholar]
  10. Goodfellow M..( 1986;). Genus Rhodococcus Zopf 1891, 28AL. . In Bergey’s Manual of Systematic Bacteriology,vol. 2 pp. 1472–1481. Edited by Sneath P. H. A., Mair N. S., Sharpe N. E., Holt J. G.. Baltimore:: Williams &Wilkins;.
    [Google Scholar]
  11. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N..( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol24:54–63. [CrossRef]
    [Google Scholar]
  12. Guan T., Zhao K., Xia Z., Lu L., Zhang X..( 2010;). Comparison of isolation media for actinobacteria from different saline environments in Xinjiang, China. . Chin J Appl Environm Biol16:429–431. [CrossRef]
    [Google Scholar]
  13. Hu H., Lin H. P., Xie Q., Li L., Xie X. Q., Hong K..( 2012;). Streptomyces qinglanensis sp. nov., isolated from mangrove sediment. . Int J Syst Evol Microbiol62:596–600. [CrossRef][PubMed]
    [Google Scholar]
  14. Kämpfer P., Huber B., Buczolits S., Thummes K., Grün-Wollny I., Busse H. J., Wollny I. G..( 2008;). Streptomyces specialis sp. nov. . Int J Syst Evol Microbiol58:2602–2606. [CrossRef][PubMed]
    [Google Scholar]
  15. Kelly K. L..( 1964;). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  16. Kim H. J., Lee S. C., Hwang B. K..( 2006;). Streptomyces cheonanensis sp. nov., a novel Streptomycete with antifungal activity. . Int J Syst Evol Microbiol56:471–475. [CrossRef][PubMed]
    [Google Scholar]
  17. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol62:716–721. [CrossRef][PubMed]
    [Google Scholar]
  18. Rong X., Huang Y..( 2010;). Taxonomic evaluation of the Streptomyces griseus clade using multilocus sequence analysis and DNA–DNA hybridization, with proposal to combine 29 species and three subspecies as 11 genomic species. . Int J Syst Evol Microbiol60:696–703. [CrossRef][PubMed]
    [Google Scholar]
  19. Saitou N., Nei M..( 1987;). The neighbour-joining method: a new method for reconstructing phylogenetic trees. . Mol Bio Evol4:406–425.
    [Google Scholar]
  20. Sasser M..( 1990;). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, , Technical Note 101.. Newark:: Microbial ID;.
    [Google Scholar]
  21. Shirling E. B., Gottlieb D..( 1966;). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol16:313–340. [CrossRef]
    [Google Scholar]
  22. Sierra G..( 1957;). A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates. . Antonie van Leeuwenhoek23:15–22. [CrossRef][PubMed]
    [Google Scholar]
  23. Staneck J. L., Roberts G. D..( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol28:226–231.[PubMed]
    [Google Scholar]
  24. Tamaoka J., Komagata K..( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett25:125–128. [CrossRef]
    [Google Scholar]
  25. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol30:2725–2729. [CrossRef][PubMed]
    [Google Scholar]
  26. Tang S. K., Wang Y., Chen Y., Lou K., Cao L. L., Xu L. H., Li W. J..( 2009;). Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. . Int J Syst Evol Microbiol59:2025–2031. [CrossRef][PubMed]
    [Google Scholar]
  27. Tatar D., Guven K., Spröer C., Klenk H. P., Sahin N..( 2014;). Streptomyces iconiensis sp. nov. and Streptomyces smyrnaeus sp. nov., two halotolerant actinomycetes isolated from a salt lake and saltern. . Int J Syst Evol Microbiol64:3126–3133. [CrossRef][PubMed]
    [Google Scholar]
  28. Waksman..( 1967;). The Actinomycetes a Summary of Current Konwledge. New York:: Ronald Press;.
    [Google Scholar]
  29. Waksman S. A., Henrici A. T..( 1943;). The nomenclature and classification of the Actinomycetes. . J Bacteriol46:337–341.[PubMed]
    [Google Scholar]
  30. Williams S. T., Davies F. L..( 1967;). Use of scanning electron microscope for the examination of actinomycetes. . J Gen Microbiol48:171–177. [CrossRef][PubMed]
    [Google Scholar]
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