@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001464, author = "Wen, Zhi-Qiang and Chen, Bingzhi and Li, Xiao and Li, Bing-Bing and Li, Cheng-Huan and Huang, Qing-Hua and Zhang, Qi-Hui and Dai, Wei-hao and Jiang, Yu-Ji", title = "Streptomyces tremellae sp. nov., isolated from a culture of the mushroom Tremella fuciformis", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "12", pages = "5028-5033", doi = "https://doi.org/10.1099/ijsem.0.001464", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001464", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Streptomyces", keywords = "a novel actinomycete", keywords = "Tremella fuciformis", abstract = "A novel actinomycete strain, designated Js-1T, was isolated from Tremella fuciformis collected from Gutian, Fujian Province, in southeastern China. The taxonomic status of this strain was determined by a polyphasic approach, which demonstrated that the novel strain was a member of the genus Streptomyces . The cell walls of this strain were found to contain ll-diaminopimelic acid, muramic acid and glycine. An analysis of whole-cell hydrolysates revealed that no characteristic sugar was present. The key identified menaquinones were MK-9 (H6) and MK-9 (H8), while the diagnostic polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylmethylethanolamine and phosphatidylglycerol. The main cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, C16 : 0 and iso-C16 : 0. An analysis of an almost complete 16S rRNA gene sequence showed that the strain shared the highest levels of sequence similarity with Streptomyces sannanensis KC-7038T (97.87 %), Streptomyces hebeiensis YIM 001T (97.84 %), Streptomyces pathocidini NBRC 13812T (97.80 %), Streptomyces cocklensis BK168T (97.25 %), Streptomyces coerulescens NBRC 12758T (97.12 %), Streptomyces aurantiogriseus NBRC 12842T (97.06 %) and Streptomyces rimosus subsp. rimosus ATCC 10970T (97.04 %). The DNA G+C content of the genomic DNA of strain Js-1T was 70.1 mol%. Furthermore, DNA–DNA hybridization tests revealed that the relatedness values between strain Js-1T and the most closely related species ranged from 15.10 to 47.20 %. Based on its phenotypic and genotypic characteristics, strain Js-1T (=CCTCC M 2011365T=JCM 30846T) is considered to represent a novel species within the genus Streptomyces , which we classified as Streptomyces tremellae sp. nov.", }