1887

Abstract

A chemo-organotrophic iodide (I)-oxidizing bacterial strain, C-3, isolated from natural gas brine of an iodine recovery facility in Kujukuri, Chiba, Japan, was characterized for representation of a novel species in the class . Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the nearest neighbours of strain C-3 were members of the genera , , and with 88–91 % sequence similarity. Cells of strain C-3 were aerobic, Gram-staining-negative, non-sporulating and rod-shaped (1.3–3.6 µm in length). Strain C-3 grew optimally at 30 °C, pH 7.5 and with 3 % NaCl (w/v). Iodide oxidation to form molecular iodine (I) was a unique trait for strain C-3, whereas the strain did not utilize iodide as a sole electron donor for chemolithoautotrophic growth. The major isoprenoid quinone was Q-10. The major cellular fatty acids were C 7 and C 5. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified aminolipids. The G+C content of the genomic DNA was 58.5 mol%. Iodide oxidation and the major cellular fatty acids composition distinguished strain C-3 from phylogenetically related bacteria. On the basis of the phenotypic features and the phylogenetic position, a novel genus and species are proposed for strain C-3 (=JCM 17843=LMG 28660), to be named gen. nov., sp. nov. We also propose to place the distinct sublineages of the genera gen. nov. and in the orders ord. nov. and ord. nov., respectively, because these genera are located far apart from the order and form the distinct lineage in the class .

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2016-12-01
2020-01-20
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