1887

Abstract

The taxonomic position of a novel actinomycete, strain TRM 46250, isolated from the sediment of a salt water beach at Baicheng, Xinjiang, China, was determined by a polyphasic approach. Strain TRM 46250 grew optimally in the presence of 2 % (w/v) NaCl and an optimum temperature range for growth of 28–37 °C. The whole-cell sugars of strain TRM 46250 were ribose, xylose, mannose and galactose. The diagnostic diamino acid was meso-diaminopimelic acid. The polar lipids were phosphatidylinositol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmethyl ethanolamine and six unidentified phospholipids. The predominant menaquinones were MK-10, MK-10(H6) and MK-10(H8). The major fatty acids were 10-methyl C18 : 0, iso-C16 : 0, C16 : 0, iso-G C16 : 1 and C18 : 1 ω9c. Based on morphological and chemotaxonomic characteristics the isolate was determined to belong to the genus Nocardiopsis . The phylogenetic tree based on its nearly complete 16S rRNA gene sequence (1493 nt) with those of representative strains showed that the strain consistently falls into a distinct phyletic line together with Nocardiopsis gilva YIM 90087 (97.68 % similarity) and a subclade consisting of Nocardiopsis composta KS9 (97.52 %), Nocardiopsis rosea YIM 90094 (97.44 %) and Nocardiopsis rhodophaea YIM 90096 (97.16 %). However, DNA–DNA hybridization studies between strain TRM 46250 and N. gilva YIM 90087 showed only 36.94 % relatedness. On the basis of these data, strain TRM 46250 should be designated as a representative of a novel species of the genus Nocardiopsis , for which the name Nocardiopsis akesuensis sp. nov. is proposed. The type strain is TRM 46250 (=CCTCC AA 2015027=KCTC 39725).

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2016-12-01
2019-10-20
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