1887

Abstract

A polyphasic approach was used to verify the novel actinomycete, strain 1SM4-01, isolated from mangrove sediment collected from Ranong Province, Thailand. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the organism was a member of the genus and was most closely related to GKU 164 (98.7 % sequence similarity), YIM 67092 (98.4 %), NEAU-Z6 (98.4 %), PT708 (98.3 %) and KC-061 (98.2 %). The strain produced branching aerial mycelium which differentiated into straight chains of rough-surfaced spores borne at the end of a short sporophore. The whole-cell hydrolysates contained -diaminopimelic acid as the diagnostic diamino acid, with madurose, mannose and ribose as the main sugars. MK-9(H) was a major menaquinone of this strain. The acyl type of peptidoglycan was -acetyl. The predominant cellular fatty acids were C 8 and iso-C. Phospholipids consisted of diphosphatidylglycerol, hydroxy-phosphatidylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, aminophospholipids and unidentified lipids. The G+C content of the genomic DNA was 70.4 mol%. On the basis of phenotypic characteristics, DNA–DNA relatedness and phylogenetic distinctiveness, strain 1SM4-01 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 1SM4-01 (=BCC 60397=NBRC 109647).

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2016-12-01
2021-10-23
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