1887

Abstract

A novel actinobacterial strain, designated Z1027, was isolated from a soil sample collected near the Tuotuo River, Qinghai–Tibet Plateau (China). The strain exhibited antibacterial activity against and . The taxonomic position of strain Z1027 was determined using a polyphasic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus and formed a distinct phyletic line in the 16S rRNA gene tree, together with ATCC 700248 (99.19 % similarity), JL-6 (98.84 %) and CFBP 4531 (98.36 %). The genomic DNA G+C content of strain Z1027 was 74±1 mol%. The DNA–DNA relatedness values between strain Z1027 and ATCC 700248 and CFBP 4531 were 38.5±0.4 and 26.2±1.2 %, respectively, both of them significantly lower than 70 %. Chemotaxonomic data revealed that strain Z1027 possessed MK-9(H) and MK-9(H) as the major menaquinones, -diaminopimelic acid as the diagnostic diamino acid and galactose as a whole-cell sugar. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatydilinositol and seven other unknown polar lipids were detected; iso-C, summed feature 3 (C 7 and/or C 6) and C were the major fatty acids. On the basis of these genotypic and phenotypic data, it is proposed that isolate Z1027 (=CGMCC 4.7272=JCM 31054) should be classified as the type strain of a novel species of the genus sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001456
2016-12-01
2021-06-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/4981.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001456&mimeType=html&fmt=ahah

References

  1. Anderson A. S., Wellington E. M. 2001; The taxonomy of Streptomyces and related genera. Int J Syst Evol Microbiol 51:797–814 [View Article][PubMed]
    [Google Scholar]
  2. Bérdy J. 2012; Thoughts and facts about antibiotics: where we are now and where we are heading. J Antibiot 65:385–395 [View Article][PubMed]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [View Article][PubMed]
    [Google Scholar]
  4. Collins M. D., Goodfellow M., Minnikin D. E., Alderson G. 1985; Menaquinone composition of mycolic acid-containing actinomycetes and some sporoactinomycetes. J Appl Bacteriol 58:77–86 [View Article][PubMed]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst bacteriol 39:224–229 [View Article]
    [Google Scholar]
  6. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  8. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 20:406–416 [CrossRef]
    [Google Scholar]
  9. Goodfellow M., Fiedler H. P. 2010; A guide to successful bioprospecting: informed by actinobacterial systematics. Antonie van Leeuwenhoek 98:119–142 [View Article][PubMed]
    [Google Scholar]
  10. Gordon R. E., Barnett D. A., Handerhan J. E., Pang H. N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the Nocardin Strain. Int J Syst Evol Microbiol 24:54–63 [View Article]
    [Google Scholar]
  11. Han X., Zheng J., Xin D., Xin Y., Wei X., Zhang J. 2015; Streptomyces albiflavescens sp. nov., an actinomycete isolated from soil. Int J Syst Evol Microbiol 65:1467–1473 [View Article][PubMed]
    [Google Scholar]
  12. He H., Liu C., Zhao J., Li W., Pan T., Yang L., Wang X., Xiang W. 2014; Streptomyces zhaozhouensis sp. nov., an actinomycete isolated from candelabra aloe (Aloe arborescens Mill). Int J Syst Evol Microbiol 64:1096–1101 [View Article][PubMed]
    [Google Scholar]
  13. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp 21–132 Edited by Munro H. N. New York: Academic Press; [CrossRef]
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  15. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2367 [View Article]
    [Google Scholar]
  16. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [View Article]
    [Google Scholar]
  17. Kämpfer P. 2012; Genus I. Streptomyces Waksman and Henrici 1943, 339AL emend. Witt and Stackebrandt 1990, 370 emend. Wellington, Stackebrandt, Sanders, Wolstrup and Jorgensen 1992, 159. In Bergey’s Manual of Systematic Bacteriology Part B, 2nd edn. Vol. 5 pp 1455–1767 Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K.-I., Ludwig W., Whitman W. B. New York: Springer; [CrossRef]
    [Google Scholar]
  18. Lechevalier M. P., Lechevalier H. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [View Article]
    [Google Scholar]
  19. Lechevalier M. P., De Bievre C., Lechevalier H. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [View Article]
    [Google Scholar]
  20. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy (Special Publication no.6) pp. 227–291 Edited by Dietz A., Thayer J. Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  21. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. 2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428 [View Article][PubMed]
    [Google Scholar]
  22. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  23. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Meth 2:233–241 [View Article]
    [Google Scholar]
  24. Montero-Calasanz M. C., Göker M., Rohde M., Spröer C., Schumann P., Busse H. J., Schmid M., Tindall B. J., Klenk H. P., Camacho M. 2013; Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium . Int J Syst Evol Microbiol 63:4386–4395 [View Article][PubMed]
    [Google Scholar]
  25. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  26. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, Technical Note 101. Newark, DE: MIDI;
    [Google Scholar]
  27. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Evol Microbiol 16:313–340 [View Article]
    [Google Scholar]
  28. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
    [Google Scholar]
  29. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  30. Waksman S. A., Henrici A. T. 1943; The nomenclature and classification of the actinomycetes. J Bacteriol 46:337–341[PubMed]
    [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. I nt J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  32. Williams S. T., Goodfellow M., Alderson G., Wellington E. M., Sneath P. H., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813 [View Article][PubMed]
    [Google Scholar]
  33. Yokota A., Tamura T., Hasegawa T., Huang L. H. 1993; Catenuloplanes japonicus gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales. Int J Syst Bacteriol 43:805–812 [View Article]
    [Google Scholar]
  34. Zhang B., Wu X., Zhang G., Li S., Zhang W., Chen X., Sun L., Zhang B., Liu G., Chen T. 2016; The diversity and biogeography of the communities of Actinobacteria in the forelands of glaciers at a continental scale. Environ Res Lett 11:054012 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001456
Loading
/content/journal/ijsem/10.1099/ijsem.0.001456
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error