1887

Abstract

This is the taxonomic study of a novel bacterial strain, designated GSS14, isolated from a sediment sample of Yuncheng Salt Lake, China. Cells were Gram-negative, ovoid to rod-shaped and motile by means of flagella. The isolate could grow at 10–45 °C, at pH 6.5–11.0 and in the presence of 0–12 % (w/v) NaCl. The dominant fatty acids were summed feature 8 (consisting of Cω7 and/or Cω6; 76.7 %) and the DNA G+C content was 61 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GSS14 was affiliated with the genus , and it was most closely related to KY 101 (98.2 % 16S rRNA gene sequence similarity) and JCM 21793 (96.6 %). DNA–DNA hybridization between strains GSS14 and KY 101 revealed 52 % relatedness. Phenotypic, chemotaxonomic and phylogenetic data support assignment of this isolate to the genus as a representative of a novel species. The name sp. nov. is proposed, with strain GSS14 (=KCTC 52186=MCCC 1K02481) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001453
2016-12-01
2020-01-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/4963.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001453&mimeType=html&fmt=ahah

References

  1. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  2. Dong X. Z., Cai M. Y.. 2001; Determinative Manual for Routine Bacteriology Beijing: Scientific Press;
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E.. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39:224–229 [CrossRef]
    [Google Scholar]
  4. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. 1994; Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Jang G. I., Hwang C. Y., Cho B. C.. 2011; Nitratireductor aquimarinus sp. nov., isolated from a culture of the diatom Skeletonema costatum, and emended description of the genus Nitratireductor. Int J Syst Evol Microbiol61:2676–2681 [CrossRef][PubMed]
    [Google Scholar]
  6. Kang H. S., Yang H. L., Lee S. D.. 2009; Nitratireductor kimnyeongensis sp. nov., isolated from seaweed. Int J Syst Evol Microbiol59:1036–1039 [CrossRef][PubMed]
    [Google Scholar]
  7. Kates M.. 1986; Techniques of Lipidology, 2nd edn. Amsterdam: Elsevier;
    [Google Scholar]
  8. Kim K. H., Roh S. W., Chang H. W., Nam Y. D., Yoon J. H., Jeon C. O., Oh H. M., Bae J. W.. 2009; Nitratireductor basaltis sp. nov., isolated from black beach sand. Int J Syst Evol Microbiol59:135–138 [CrossRef][PubMed]
    [Google Scholar]
  9. Labbé N., Parent S., Villemur R.. 2004; Nitratireductor aquibiodomus gen. nov., sp. nov., a novel alpha-proteobacterium from the marine denitrification system of the Montreal Biodome (Canada). Int J Syst Evol Microbiol54:269–273 [CrossRef][PubMed]
    [Google Scholar]
  10. Lai Q., Yu Z., Yuan J., Sun F., Shao Z.. 2011a; Nitratireductor indicus sp. nov., isolated from deep-sea water. Int J Syst Evol Microbiol61:295–298 [CrossRef][PubMed]
    [Google Scholar]
  11. Lai Q., Yu Z., Wang J., Zhong H., Sun F., Wang L., Wang B., Shao Z.. 2011b; Nitratireductor pacificus sp. nov., isolated from a pyrene-degrading consortium. Int J Syst Evol Microbiol61:1386–1391 [CrossRef][PubMed]
    [Google Scholar]
  12. Lane D. J.. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp.115–175 Edited by Stackebrandt E., Goodfellow M.. Wiley: Chichester;
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B.. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39:159–167 [CrossRef]
    [Google Scholar]
  14. Rzhetsky A., Nei M.. 1993; Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol10:1073–1095[PubMed]
    [Google Scholar]
  15. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  16. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  17. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S.. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol51:1639–1652 [CrossRef][PubMed]
    [Google Scholar]
  18. Tamaoka J., Katayama-Fujimura Y., Kuraishi H.. 1983; Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol54:31–36 [CrossRef]
    [Google Scholar]
  19. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001453
Loading
/content/journal/ijsem/10.1099/ijsem.0.001453
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error