1887

Abstract

A novel Gram-stain-negative, strictly aerobic, non-motile, rod-shaped, capsule-forming bacterium, designated strain YLT33, that formed orange-red colonies was isolated from mountain cliff soil from Enshi Grand Canyon, southwest China. Growth occurred at 4–35 °C (optimum 28 °C) and at pH 6.0–10.0 (optimum 7.0). It showed maximum (99.3 %) 16S rRNA gene sequence similarity and formed a monophyletic clade with MC 3718 (=CCTCC 2014274). The DNA G+C content was 68.5 mol% and strain YLT33 showed a 50.5 % DNA–DNA relatedness value to MC 3718. The major fatty acids (>5 %) were Cω6 (40.7 %), C (10.4 %), Cω6 (9.4 %), summed feature 3 (Cω7 and/or iso-C 2-OH; 8.6 %), Cω8 (7.1 %), Cω7 (6.1 %), and C 2-OH (5.7 %). Ubiquinone-10 was the sole respiratory quinone. The polar lipids of strain YLT33 contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, two unknown glycolipids and one unknown phospholipid. Carotenoids were present in cells. Homospermidine was the major polyamine. In addition, strain YLT33 showed obvious differences from the closely related strain MC 3718 with respect to major polar lipids, fatty acids and other morphological, physiological and biochemical characteristics. These results from polyphasic taxonomic studies reveal that strain YLT33 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YLT33 (=CCTCC AB 2015150=KCTC 42644).

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2016-12-01
2020-09-26
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