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Abstract

Lyme borreliosis (LB) is a multisystem disease caused by spirochetes in the (Bbsl) genospecies complex. We previously described a novel Bbsl genospecies (type strain MN14-1420) that causes LB among patients with exposures to ticks in the upper midwestern USA. Patients infected with the novel Bbsl genospecies demonstrated higher levels of spirochetemia and somewhat differing clinical symptoms as compared with those infected with other Bbsl genospecies. The organism was detected from human specimens using PCR, microscopy, serology and culture. The taxonomic status was determined using an eight-housekeeping-gene (, , , , , , and ) multi-locus sequence analysis (MLSA) and comparison of 16S rRNA gene, , , and nucleotide sequences. Using a system threshold of 98.3 % similarity for delineation of Bbsl genospecies by MLSA, we demonstrated that the novel species is a member of the Bbsl genospecies complex, most closely related to (94.7–94.9 % similarity). This same species was identified in ticks collected in Minnesota and Wisconsin. This novel species, sp. nov, is formally described here. The type strain, MN14-1420, is available through the Deutsche Sammlung von Mikroorganismen und Zelkulturen GmbH (DSM 102811) and the American Type Culture Collection (ATCC BAA-2743).

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2016-11-01
2024-03-28
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