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Volume 66, Issue 11

sp. nov., a hydrogenotrophic methanogen isolated from deep sedimentary aquifers in a natural gas field Free

Abstract

A mesophilic, hydrogenotrophic methanogen, designated strain MobH, was isolated from sediments derived from deep sedimentary, natural-gas-bearing aquifers in Japan. Strain MobH utilized H/CO or formate, but not ethanol, 1-propanol, 2-propanol, 2-butanol or cyclopentanol, for growth and methane production. In addition, acetate and tungsten were required for growth. Yeast extract stimulated the growth, but was not required. The cells were weakly motile with multiple flagella, presented as a curved-rod-shaped (0.8×2.0 µm) and occurred singly or in pairs. Strain MobH grew at 15–40 °C (optimum 35 °C) and at pH 5.9–7.9 (optimum pH 7.0–7.5). The sodium chloride range for growth was 0–5.8 % (optimum 2 %). The G+C content of the genomic DNA was 37.6 mol%. In the phylogenetic tree based on the 16S rRNA gene sequences, strain MobH clustered together with (95.4 % in sequence similarity), and formed a distinct clade from SEBR 4847 (95.6 %) and G-2000 (95.4 %). The two species of the genus utilized 2-propanol, whereas strain MobH and , the sole species of the genus , do not. Based on phenotypic and phylogenetic features, we propose a novel species for the isolate with the name, sp. nov. The type strain is MobH (=DSM 21220=NBRC 104160).

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Keyword(s): ancient sea water , deep subsurface , gas field , methanogen and Methanogenesis
© 2016 IUMS
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2016-11-01
2024-04-18
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Methanomicrobium antiquum sp. nov., a hydrogenotrophic methanogen isolated from deep sedimentary aquifers in a natural gas field
Int J Syst Evol Microbiol 66, 4873 (2016); https://doi.org/10.1099/ijsem.0.001444
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