1887

Abstract

A novel Gram-reaction-positive actinobacterium, designated BMG 804, was isolated from an archaeological Roman pool located in Gafsa, Tunisia. The strain grew as dry bright orange colonies at 30 °C and pH 6.0–8.0. It contained -diaminopimelic acid in the cell wall. The whole-cell sugars consisted of glucose, rhamnose and ribose. Polar lipids present were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, an unidentified glycolipid and two unidentified phospholipids. MK-9(H) was the predominant menaquinone. The fatty acid profile contained major amounts (>5 %) of Cω8, Cω7, iso-C, iso-C and iso-CH. The 16S rRNA gene sequence of BMG 804 showed 99.4 % as highest sequence similarity with . DNA–DNA hybridization between strain BMG 804 and DSM 44509 was 48.6±6.6 %. The G+C content of the DNA was 73.7 mol%. On the basis of the phenotypic and genotypic characteristics, including DNA–DNA hybridization results, BMG 804 (=DSM 46835=CECT 8876) is proposed as the type strain of a novel species sp. nov. Emended descriptions of the genus and the species , , and are also proposed.

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2016-11-01
2020-08-11
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