%0 Journal Article %A Ngaemthao, Wipaporn %A Chunhametha, Suwanee %A Suriyachadkun, Chanwit %T Actinoplanes subglobosus sp. nov., isolated from mixed deciduous forest soil %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 11 %P 4850-4855 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001440 %K Actinobacteria %K Actinoplanes subglobosus %K Thailand %I Microbiology Society, %X A novel filamentous bacterial strain, A-T 5400T, which developed subglobose sporangia at the end of sporangiophores on substrate mycelia, was isolated from mixed deciduous forest soil collected in Thailand. The taxonomic position of this micro-organism was described using a polyphasic approach. The 16S rRNA gene sequence and phylogenetic analysis indicated that strain A-T 5400T belonged to the genus Actinoplanes and was most closely related to ‘ Actinoplanes hulinensis ' NEAU-M9 (98.82 % 16S rRNA gene sequence similarity) and Actinoplanes philippinensis NBRC 13878T (98.75 %). The DNA–DNA relatedness values that distinguished the novel strain from the closest species were below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars were ribose, galactose, glucose and xylose. The predominant menaquinone was MK-9(H4). The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were unsaturated fatty acids C16 : 1, branched fatty acids iso-C16 : 0 and iso-C15 : 0. The G+C content of the genomic DNA was 71 mol%. Following evidence from phenotypic, chemotaxonomic and genotypic studies, the new isolate is proposed to represent a novel species of the genus Actinoplanes named Actinoplanes subglobosus sp. nov. The type strain is A-T 5400T (=BCC 42734T=TBRC 5832T=NBRC 109645T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001440