1887

Abstract

A Gram-stain-negative, facultatively anaerobic, motile and rod-shaped strain, designed H2, was isolated from the Western Pacific Ocean, and subjected to a taxonomic investigation using a polyphasic approach. Strain H2 grew at 15–40 °C and pH 6.0–9.0 (optimum 37 °C and pH 6.5), and with 1–10 % (w/v) NaCl (optimum 2 %). The predominant respiratory quinone was ubiquinone-10 (Q-10) and the major fatty acids identified were C cyclo 8, C 7, C and 11-methyl-C 7. The polar lipids of strain H2 consisted of phosphatidylglycerol, one unknown phospholipid, one unknown glycolipid and three unidentified aminolipids. The DNA G+C content was 75.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain H2 formed a distinct clade belong to the family within the . On the basis of morphological, physiological and chemotaxonomic characteristics, together with the results of phylogenetic analysis, strain H2 represents a novel species in a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is H2(=MCCC 1K02279=KCTC 42964).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001439
2016-11-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/11/4844.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001439&mimeType=html&fmt=ahah

References

  1. Amoozegar M. A., Makhdoumi-Kakhki A., Ramezani M., Nikou M. M., Fazeli S. A., Schumann P., Ventosa A. 2013; Limimonas halophila gen. nov., sp. nov., an extremely halophilic bacterium in the family Rhodospirillaceae. Int J Syst Evol Microbiol 63:1562–1567 [View Article][PubMed]
    [Google Scholar]
  2. Balcázar J. L., Planas M., Pintado J. 2012; Oceanibacterium hippocampi gen. nov., sp. nov., isolated from cutaneous mucus of wild seahorses (Hippocampus guttulatus). Antonie van Leeuwenhoek 102:187–191 [View Article][PubMed]
    [Google Scholar]
  3. Batut J., Andersson S. G., O'Callaghan D. 2004; The evolution of chronic infection strategies in the alpha-proteobacteria. Nat Rev Microbiol 2:933–945 [View Article][PubMed]
    [Google Scholar]
  4. Coenye T., Goris J., Spilker T., Vandamme P., LiPuma J. J. 2002; Characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of Inquilinus limosus gen. nov., sp. nov. J Clin Microbiol 40:2062–2069 [View Article][PubMed]
    [Google Scholar]
  5. Dong X. Z., Cai M. Y. 2001 Determinative Manual for Routine Bacteriology, 1st edn. Beijing: Scientific Press (English translation);
    [Google Scholar]
  6. Fang M. X., Zhang W. W., Zhang Y. Z., Tan H. Q., Zhang X. Q., Wu M., Zhu X. F. 2012; Brassicibacter mesophilus gen. nov., sp. nov., a strictly anaerobic bacterium isolated from food industry wastewater. Int J Syst Evol Microbiol 62:3018–3023 [View Article][PubMed]
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  8. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 20:406–416 [View Article]
    [Google Scholar]
  9. Garrity G., Bell J., Lilburn T. 2005; Order I. Rhodospirillales Pfennig and Trüper 1971, 17AL. In Bergey’s Manual of Systematic Bacteriology, 2nd edn., vol. 2. the Proteobacteria, Part C, the Alpha-, Beta-, Delta-, and Epsilonproteobacteria pp. 1–95 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer; [CrossRef]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  11. Kim S. B., Falconer C., Williams E., Goodfellow M. 1998; Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil. Int J Syst Bacteriol 48:59–68 [View Article][PubMed]
    [Google Scholar]
  12. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Meth Microbiol 19:161–207 [CrossRef]
    [Google Scholar]
  13. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. 1998; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 38:358–361 [View Article]
    [Google Scholar]
  14. Labrenz M., Tindall B. J., Lawson P. A., Collins M. D., Schumann P., Hirsch P. 2000; Staleya guttiformis gen. nov., sp. nov. and Sulfitobacter brevis sp. nov., alpha-3-Proteobacteria from hypersaline, heliothermal and meromictic antarctic Ekho Lake. Int J Syst Evol Microbiol 50:303–313 [View Article][PubMed]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  16. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  17. Pan J., Sun C., Zhang X. Q., Huo Y. Y., Zhu X. F., Wu M. 2014; Paracoccus sediminis sp. nov., isolated from pacific ocean marine sediment. Int J Syst Evol Microbiol 64:2512–2516 [View Article][PubMed]
    [Google Scholar]
  18. Pfennig N., Trüper H. G. 1971; Higher taxa of the phototrophic bacteria. Int J Syst Bacteriol 21:17–18 [View Article]
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  20. Sorokina A. Y., Chernousova E. Y., Dubinina G. A. 2012; Ferrovibrio denitrificans gen. nov., sp. nov., a novel neutrophilic facultative anaerobic Fe(II)-oxidizing bacterium. FEMS Microbiol Lett 335:19–25 [View Article][PubMed]
    [Google Scholar]
  21. Sun C., Pan J., Zhang X. Q., Su Y., Wu M. 2015; Pseudoroseovarius zhejiangensis gen. nov., sp nov., a novel alpha-proteobacterium isolated from the chemical wastewater, and reclassification of Roseovarius crassostreae as Pseudoroseovarius crassostreae comb. nov., Roseovarius sediminilitoris as Pseudoroseovarius sediminilitoris comb. nov. and Roseovarius halocynthiae as Pseudoroseovarius halocynthiae comb. nov. Antonie van Leeuwenhoek 108:291–299 [CrossRef]
    [Google Scholar]
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  23. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn. pp. 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  24. Williama S. T., Davies F. L. 1965; Use of antibiotics for selective isolation and enumeration of actinomycetes in soil. J Gen Microbiol 38:251–261 [View Article]
    [Google Scholar]
  25. Xi X. D., Dong W. L., Zhang J., Huang Y., Cui Z. L. 2013; Taonella mepensis gen. nov., sp. nov., a member of the family Rhodospirillaceae isolated from activated sludge. Int J Syst Evol Microbiol 63:2472–2476 [View Article][PubMed]
    [Google Scholar]
  26. Xie C. H., Yokota A. 2005; Pleomorphomonas oryzae gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from paddy soil of Oryza sativa. Int J Syst Evol Microbiol 55:1233–1237 [View Article][PubMed]
    [Google Scholar]
  27. Zhang W. Y., Huo Y. Y., Zhang X. Q., Zhu X. F., Wu M. 2013; Halolamina salifodinae sp. nov. and Halolamina salina sp. nov., two extremely halophilic archaea isolated from a salt mine. Int J Syst Evol Microbiol 63:4380–4385 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001439
Loading
/content/journal/ijsem/10.1099/ijsem.0.001439
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error