1887

Abstract

A novel actinobacterial strain, designated MB27, was isolated from a Saharan soil sample collected in Mzab region (Ghardaïa province, South Algeria). Strain MB27 was characterized following a polyphasic taxonomic approach. This strain produced a branched and fragmented substrate mycelium, which was found to have a yellowish orange colour. A white scanty aerial mycelium was produced on most media tested. Chemotaxonomic and phylogenetic studies clearly demonstrated that strain MB27 belongs to the family and is closely related to the genus . Cell-wall hydrolysates contained -diaminopimelic acid but not glycine, and whole-cell hydrolysates contained galactose, glucose, ribose and small amounts of mannose and rhamnose. The detected phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. Mycolic acids were not detected while the predominant fatty acid was iso-branched hexadecanoate (iso-C). The major menaquinone was MK-9(H). Results of 16S rRNA gene sequence comparisons revealed that strain MB27 shairs the highest degree of similarity with DSM 45486 (99.8%), DSM 45457 (99.3 %), DSM 43749 (98.6 %) and DSM 45665 (98.6 %). However, it exhibited only 11-42 % DNA–DNA relatedness to the neighbouring species. On the basis of phenotypic characteristics, 16S rRNA gene sequence comparisons and DNA–DNA hybridization, strain MB27 is shown to represent a novel species of the genus , for which the name sp. nov. (type strain MB27=DSM 46885=CECT 9045) is proposed.

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2016-11-01
2022-01-21
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