1887

Abstract

A novel actinomycete, designated MI503-A4, was isolated from soil. Comparative analysis of 16S rRNA gene sequences indicated that MI503-A4 was phylogenetically related to members of the family . The most closely related genus was (95.7–96.2 % sequence similarity). Substrate mycelia were branched and pale yellow to pale yellowish-brown. Straight- to zigzag-shaped aerial mycelia were observed, but Sporangium-like structures were absent. The whole-cell hydrolysate contained -diaminopimelic acid. The muramic acid residues in the peptidoglycan were -acetylated. Whole-cell sugars were rhamnose, ribose, arabinose and galactose (cell wall chemotype IV). The predominant menaquinone was MK-9(H). A small amount of MK-8(H) was also detected. The DNA G+C content was 70.3–71.1 mol%. Polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine and hydroxyl-phosphatidylethanolamine. Cellular fatty acid analysis of MI503-A4 detected predominantly iso-C (11.5 %), iso-C (13.3 %) and iso-C (35.7 %). Phenotypic and phylogenetic characteristics differentiated MI503-A4 from members of all genera within the family with validly published names. Therefore, MI503-A4 is proposed to be a representative of a novel species in a novel genus, gen. nov., sp. nov. The type strain of the type species is MI503-A4 (=NBRC 109632=DSM 45934).

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2016-11-01
2022-01-21
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