1887

Abstract

A novel Gram-stain-positive, strictly aerobic, endospore-forming, rod-shaped bacterial strain 7578-24 was isolated from ripened Pu’er tea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 7578-24 clustered with species of the genus in the family with 97.8–95.2 % sequence similarities, and was most closely related to YN3 with 97.8 % 16S rRNA gene sequence similarity. The DNA–DNA relatedness value between strain 7578-24 and YN3 was 35 %. Strain 7578-24 had a cell-wall type A1γ peptidoglycan with -diaminopimelic acid as the diagnostic diamino acid. The major menaquinone was menaquinone 7 (MK-7). C 7 (45.4 %), anteiso-C (30.6 %) and anteiso-C (10.1 %) were the predominant fatty acids, and diphosphatidylglycerol, phosphatidylglycerol, five unknown phospholipids and one unknown aminolipid were the major polar lipids. The DNA G+C content of strain 7578-24 was 45.2 mol%. Strain 7578-24 could be differentiated from other related species of the genus based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA–DNA hybridization data. On the basis of polyphasic evidence from this study, a novel species of the genus named sp. nov. is proposed, with strain 7578-24 (=CGMCC 1.15371=JCM 31236) as the type strain.

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2016-11-01
2022-01-21
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