1887

Abstract

A novel Gram-stain-positive, strictly aerobic, endospore-forming, rod-shaped bacterial strain 7578-24 was isolated from ripened Pu’er tea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 7578-24 clustered with species of the genus in the family with 97.8–95.2 % sequence similarities, and was most closely related to YN3 with 97.8 % 16S rRNA gene sequence similarity. The DNA–DNA relatedness value between strain 7578-24 and YN3 was 35 %. Strain 7578-24 had a cell-wall type A1γ peptidoglycan with -diaminopimelic acid as the diagnostic diamino acid. The major menaquinone was menaquinone 7 (MK-7). C 7 (45.4 %), anteiso-C (30.6 %) and anteiso-C (10.1 %) were the predominant fatty acids, and diphosphatidylglycerol, phosphatidylglycerol, five unknown phospholipids and one unknown aminolipid were the major polar lipids. The DNA G+C content of strain 7578-24 was 45.2 mol%. Strain 7578-24 could be differentiated from other related species of the genus based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA–DNA hybridization data. On the basis of polyphasic evidence from this study, a novel species of the genus named sp. nov. is proposed, with strain 7578-24 (=CGMCC 1.15371=JCM 31236) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001424
2016-11-01
2020-01-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/11/4760.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001424&mimeType=html&fmt=ahah

References

  1. De Ley J., Cattoir H., Reynaerts A.. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem12:133–142 [CrossRef][PubMed]
    [Google Scholar]
  2. Doetsch R.. 1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology pp21–23 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  3. Dong X.-Z., Cai M.-Y.. 2001; Determinative Manual for Routine Bacteriology Beijing: Scientific Press;
    [Google Scholar]
  4. Fitch W. M.. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool20:406–416 [CrossRef]
    [Google Scholar]
  5. Hatayama K., Shoun H., Ueda Y., Nakamura A.. 2006; Tuberibacillus calidus gen. nov., sp. nov., isolated from a compost pile and reclassification of Bacillus naganoensis Tomimura et al. 1990 as Pullulanibacillus naganoensis gen. nov., comb. nov. and Bacillus laevolacticus Andersch et al. 1994 as Sporolactobacillus laevolacticus comb. nov. Int J Syst Evol Microbiol56:2545–2551 [CrossRef][PubMed]
    [Google Scholar]
  6. Huss V. A., Festl H., Schleifer K. H.. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol4:184–192 [CrossRef][PubMed]
    [Google Scholar]
  7. Jukes T. H., Cantor C. R.. 1969; Evolution of the protein molecules. In Mammalian Protein Metabolism pp21–132 Edited by Munro H. N.. New York: Academic Press;[CrossRef]
    [Google Scholar]
  8. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E.. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol38:358–361 [CrossRef]
    [Google Scholar]
  9. Lechevalier M. P., Lechevalier H. A.. 1980; The chemotaxonomy of actinomyces. In Actinomycete Taxonomy, Special Publication 6 , pp.227–291 Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  10. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. et al. 2009; Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol59:2114–2121 [CrossRef][PubMed]
    [Google Scholar]
  11. Marmur J., Doty P.. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  12. Miller L. T.. 1982; Single derivation method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol16:584–586[PubMed]
    [Google Scholar]
  13. Niu L.-L., Song L., Dong X.-Z.. 2008; Proteiniborus ethanoligenes gen. nov., sp. nov., an anaerobic protein-utilizing bacterium. Int J Syst Evol Microbiol58:12–16 [CrossRef][PubMed]
    [Google Scholar]
  14. Niu L.-L., Tang T.-Y., Song L., Xiong M.-J., Tian J.-Q., Zhang K.-G., Hu X., Zhu D.-C.. 2015; Pullulanibacillus pueri sp. nov., isolated from Pu'er tea. Int J Syst Evol Microbiol65:2167–2171 [CrossRef][PubMed]
    [Google Scholar]
  15. Pereira S. G., Albuquerque L., Nobre M. F., Tiago I., Veríssimo A., Pereira A., da Costa M. S.. 2013; Pullulanibacillus uraniitolerans sp. nov., an acidophilic, U(VI)-resistant species isolated from an acid uranium mill tailing effluent and emended description of the genus Pullulanibacillus. Int J Syst Evol Microbiol63:158–162 [CrossRef][PubMed]
    [Google Scholar]
  16. Rhuland L. E., Work E., Denman R. F., Hoare D. S.. 1955; The behavior of the isomers of α,ε-diaminopimelic acid on paper chromatograms. J Am Chem Soc77:4844–4846 [CrossRef]
    [Google Scholar]
  17. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  18. Staneck J. L., Roberts G. D.. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol28:226–231[PubMed]
    [Google Scholar]
  19. Tamura K., Nei M.. 1993; Estimation of the number of nucleotide substitutions in the control region of mitochrondrial DNA in humans and chimpanzees. Mol Biol Evol10:512–526[PubMed]
    [Google Scholar]
  20. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  22. Tindall B. J.. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol13:128–130 [CrossRef]
    [Google Scholar]
  23. Tindall B. J.. 1990b; Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett66:199–202 [CrossRef]
    [Google Scholar]
  24. Tindall B. J., Sikorski J., Smibert R. M., Kreig N. R.. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn. pp330–393 Edited by Reddy C. A., Beveridge T. J., Brezmal J. A., Marzluf G., Schmidt T. M., Snyder L. R.. Washington, DC: ASM Press;
    [Google Scholar]
  25. Tomimura E., Zeman N. W., Frankiewicz J. R., Teague W. M.. 1990; Description of Bacillus naganoensis sp. nov. Int J Syst Bacteriol40:123–125 [CrossRef][PubMed]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37:463–464[CrossRef]
    [Google Scholar]
  27. Winker S., Woese C. R.. 1991; A definition of the domains Archaea, Bacteria and Eucarya in terms of small subunit ribosomal RNA characteristics. Syst Appl Microbiol14:305–310 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001424
Loading
/content/journal/ijsem/10.1099/ijsem.0.001424
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error