1887

Abstract

A Gram-staining-negative, aerobic, motile, rod-shaped, catalase- and oxidase-negative strain with one polar flagellum, designated THG-YS3.6, was isolated from rhizosphere soil of a mugunghwa flower collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10–37 °C (optimum 25–30 °C), at pH 6–8 (optimum 7.0) and with 0–2.0 % NaCl (optimum 1 %). The isoprenoid quinone was ubiquinone-8 (Q–8). The major cellular fatty acids were iso-C, iso-C 3-OH, iso-C, iso-C, Cω7 alcohol, C, iso-C and summed feature 9 (iso-Cω9 and/or C 10-methyl). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unknown phospholipid, one unknown lipid and three unknown aminolipids. The DNA G+C content of strain THG-YS3.6 was 65.3 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-YS3.6 were identified as KACC 11407 (98.7 %), KCTC 22249 (98.0 %), KACC 11587 (97.6 %) and KACC 17646 (97.1 %). The DNA–DNA relatedness values between strain THG-YS3.6 and KACC 11407, KCTC 22249, KACC 11587 and KACC 17646 were 53.8±1.0 %, 12.9±1.2 %, 10.9±0.6 % and 7.0±1.9 %, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain THG-YS3.6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is THG-YS3.6 (=KCTC 52082=CCTCC AB 2015358).

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2016-11-01
2022-01-23
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